미니돼지 정액 보존에 있어서 해양성 광천수의 영향
Effects of Brine Mineral Water on Sperm Preservation in Miniature Pig
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초록
The objective of this study was to evaluate the sperm liquid storage diluted with Brine Mineral Water (BMW) in miniature pig. Therefore we performed to find optimal concentration of BMW. The ejaculated semen from miniature pig was collected by gloved-hand method. The collected semen was diluted with dilution solution (Mulberry Ⅲ; modified-Modena B) which BMW was added. Concentration of BMW was 0, 2.5, 5, 7.5, 10 and 12.5% in dilution solution. The diluted semen was preserved at 17℃. Sperm ability was estimated by viability, capacitation acrosome reaction using chlortetracycline (CTC), hypoosmotic swelling test (HOST), morphologic abnormality. The diluted semen was observed for 7 days. The viability was significantly measured higher at 2.5% concentration of BMW than other groups (p<0.05). The AR-pattern of CTC analysis was significantly measured lower at 12.5% concentration of BMW than other groups (p<0.05). However, abnormality was not significantly different among all the groups (p<0.05). In conclusion, viability of sperm was the highest at 2.5% concentration of BMW but BMW had a negative effect on HOST, capacitation and acrosome reaction in sperm of miniature pig.
Abstract
The objective of this study was to evaluate the sperm liquid storage diluted with Brine Mineral Water (BMW) in miniature pig. Therefore we performed to find optimal concentration of BMW. The ejaculated semen from miniature pig was collected by gloved-hand method. The collected semen was diluted with dilution solution (Mulberry Ⅲ; modified-Modena B) which BMW was added. Concentration of BMW was 0, 2.5, 5, 7.5, 10 and 12.5% in dilution solution. The diluted semen was preserved at 17℃. Sperm ability was estimated by viability, capacitation acrosome reaction using chlortetracycline (CTC), hypoosmotic swelling test (HOST), morphologic abnormality. The diluted semen was observed for 7 days. The viability was significantly measured higher at 2.5% concentration of BMW than other groups (p<0.05). The AR-pattern of CTC analysis was significantly measured lower at 12.5% concentration of BMW than other groups (p<0.05). However, abnormality was not significantly different among all the groups (p<0.05). In conclusion, viability of sperm was the highest at 2.5% concentration of BMW but BMW had a negative effect on HOST, capacitation and acrosome reaction in sperm of miniature pig.
참고문헌 (13)
- 정갑택, "해양심층수의 특성과 이용 및 국내외 연구현황", 한국식품영양학회지, 21(4), pp.592~598 (2008), 한국식품영양학회
- 김완재, "해양성 광천수의 암세포 성장, 전이 및 신생 혈관 생성 억제 효과", 한국식품영양학회지, 22(4), pp.542~547 (2009), 한국식품영양학회
- Marin, G. J., "Effect of dietary selenium and vitamin E on spermatogenic development in boars", J. Anim. Sci, 78, pp.1537~1543 (2000)
- Marin, G. J., "Effects of dietary seleniun and vitamin E on boar performance and tissue responses, semen quality, and subsequent fertilization rates in mature gilts", J. Anim. Sci, 75, pp.2994~3003 (2007)
- Maxwell, W. M. C., "Membrane status of boar spermatozoa after cooling or cryopreservation", Therogenology, 48, pp.209~219 (1997)
- Pursel, V. G., "Acrosome morphology of boar spermatozoa incubated before col shock", J. Anim Sci, 34, pp.273~278 (1972)
- Shalini, S., "Dietary selenium deficiency as well as excess supplementation induces multiple defects in mouse epididymal spermatozoa: understanding the role of selenium in male fertility", Int. J. Androl, 31, pp.438~449 (2008)
- Shi, L. G., "Effect of elemental nano-seleniun on semen quality, glutathione peroxidase activity, and testis ultrastructure in male boer goats", Anim. Rep. Sci, 118, pp.248~254 (2010)
- Silva, P. F. N., "Detection of damage in mammalian sperm cells", Theriogenology, 65, pp.958~978 (2006)
- Edith Wallace, "Effects of selenium deficiency on the shape and arrangement of rodent sperm mitochondria", Gamete Res, 4, pp.389~399 (1983)
- Wang, W. H., "Functional analysis using chlortetracycline fluorescence and in vitro fertilization of frozen thawed ejaculated boar spermatozoa incubated in protein- free chemically defined medium", J. Reprod. Fertil, 104, pp.305~313 (1995)
- 유승훈, "산업연관분석을 이용한 해양심층수 산업화의 국민경제적 파급효과 분석", 산업경제연구, 20(4), pp.1345~1357 (2007), 한국산업경제학회
- 이규승, "동물생식공학" (2007), 선진문화사