섬백리향과 백리향 70% 에탄올 추출물의 미백효과에 관한 연구
A Study on Whitening effect of 70% Ethanol Extract from Thymus mmagnus and Thymus quinquecostatus
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섬백리향과 백리향 70% 에탄올 추출물의 미백에 관한 연구 결과 melanoma 세포주 B16F10를 이용한 세포 생존율 실험에서 섬백리향과 백리향 70% 에탄올 추출물 모두 멜라닌 형성 억제 효과가 있는 것으로 나타났다. 이러한 미백 효능은 melanin에 대한 tyrosinase의 활성을 직접적으로 저해하기 보다는 α-MSH에 의해서 자극되는 전사인자인 MITF의 발현을 억제함으로써, MITF에 의해 촉진되는 tyrosinase의 발현 자체를 저해함으로써 나타난다는 것을 실험을 통해 확인하였다. 이러한 결과로 보아 섬백리향과 백리향 70% 에탄올 추출물이 우수한 미백 화장품 활성 소재로의 가치가 높음을 보여준다.
Abstract
This study confirmed that the tyrosinase enzyme activity is inhibited in two extracts as a result of the experiment on the whitening of the 70% ethanol extract from Thymus mmagnus and Thymus quinquecostatus. In the test of cell viability using Melanoma cell-line B16F10, more than 80% of cell viability was identified in the concentration of 100 μg/ml of the 70% ethanol extract from Thymus mmagnus and Thymus quinquecostatus. In the measurement of the melanin biosynthesis inhibitory activity in B16F10 cell, the inhibitory effect on Melanogenesis with 11.09%, 17.16% was verified in the concentration of 100 μg/ml of two extracts. In the 70% ethanol extract from Thymus mmagnus and Thymus quinquecostatus, the inhibitory efficacy of melanin biosynthesis and secretion was inspected to be shown by the inhibition of tyrosinase expression through hindering MITF as well as directly impeding the tyrosinase enzyme activity. The aim is to suggest applicability as the cosmetic material through experimenting the whitening effect of the 70% ethanol extract from Thymus mmagnus and Thymus quinquecostatus.
참고문헌 (15)
- 김성민, "섬백리향 정유의 진통, 항염증, 정신적인 작용", 약 학 회 지, 51(6), pp.508~516 (2007), 대한약학회
- 김수은, "Alleviating Effects of Cnidium officinale Water Extract on Skin Barrier and Inflammation in Hairless Mouse", Journal of Investigative Cosmetology, 7(2), pp.137~145 (2011), The Korean Society for Investigative Cosmetology[DOI]
- 박희두, "울릉도의 자연지리", 국토지리학회지, 31(1), pp.27~40 (1997)
- 신승원, "Thymus quinquecostatus 와 T. magnus의 에센셜 오일의 항진균 활성", Planta Med, 70(11), pp.1090~1092 (2004)
- 이웅, "울릉도와 독도 자생식물의 생물지리학적 연구", 박사, pp.2016
- M. L. Faleiro, "Antimicrobial activity of essential oils isolated from Portuguese endemic species of Thymus", Letters in Applied Microbiology, 36, pp.35~40 (2003)
- L. Giamperi, "The in vitro action of essential oils on different organisms", Journal of Essential Oil Research, 14(4), pp.312~318 (2002)
- M. Hudaib, "GC", MS evaluation of thyme(Thymus vulgaris L.) oil composition and variations during the vegetative cycle, 2002, pp.29(4), 691~700
- J. Kitajima, "Monoterpenoids and their glycosides from the leaf of thyme", Phytochemistry, 65(24), pp.3279~3287 (2004)
- I. Rasooli, "Growth inhibition and morphological alterations of Aspergillus niger by essential oils from Thymus eriocalyx and Thymus x-porlock", Food Control, 17(5), pp.359~364 (2006)
- A. Rustaiyan, "Volatile constituents of three Thymus species grown wild in Iran", Planta Medica, 66(2), pp.197~198 (2000)
- Seungwon Shin, "In Vitro Inhibitory Activities of Essential Oils from Two Korean Thymus species against Antibiotic-Resistant Pathogens", Archives of Pharmacal Research, 28(8), pp.897~901 (2005), 대한약학회
- A. Yagi, "Inhibition of Mushroom-Tyrosinase by Aloe Extract", Planta Medica, 53(6), pp.515~517 (1987)
- "국가생물종지식정보시스템"[URL]