카르노스산의 멜라닌생성 억제활성
Inhibitory Effects of Carnosic Acid on Melanin Synthesis
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목적: 본 연구는 카르노스산이 B16F1 세포의 미백 효과를 확인하기 위하여 수행되었다. 방법: MSH에 의해 유도된 B16F1 세포의흑화가 카르노스산에 의해 완화되는지 여부를 확인하기 위해서 카르노스산의 농도별 세포 생존률, 타이로시나제 활성도 억제, 멜라닌 생성을 유도하는 mRNA의 발현 양상, 멜라닌 단백질의 생성 정도를 확인하였다. 결과: 카르노스산의 미백 효과를 확인하기 위하여 타이로시나제 활성도를 측정한 결과, 10 μg/ml의 카르노스산을 처리한 조건에서 50.3%의 타이로시나제 활성도를 억제하는효과를 보였다. 또한 TYRP1/TYRP2/TYR/MITF 의 유전자 발현을 확인한 결과, MSH에 의해 증가한 유전자의 발현이 카르노스산에 의해 각각 31.5% , 43.5% , 42% , 44% 감소하였다. B16F1 세포의 멜라닌생성에 카르노스산이 미치는 영향을 확인한 결과, 10 μg/mL의 카르노스산을 처리하였을 때 44%의 멜라닌형성 억제 효능을 보임을 확인할 수 있었다. 따라서 본 연구자는 카르노스산의미백 효능을 확인하였다. 결론: 본 연구결과를 통하여 카르노스산의 미백 효능을 확인하였고, 향후 카르노스산이 화장품 및 건강식품과 의약품의 개발에 응용될 수 있는 소재로서의 가능성을 확인하기 위해 추가적인 기전연구가 필요할 것으로 생각된
Abstract
Purpose: This research was carried out to ascertain the whitening effects of carnosic acid on a B16F1 mouse melanoma cell line. Methods: It involved quantitative assessments based on cell viability and assays of gene expression, tyrosinase enzyme activity, and melanin contents. The effects of carnosic acid on tyrosinase (TYR )/microphthalmia-associated transcription factor (MITF )/ tyrosinase-related protein 1 (TYRP1 )/TYRP2 messenger ribonucleic acid expression levels and melanin content were determined. Results: Real-time quantitative reverse transcription polymerase chain reaction (real-time qRT-PCR) showed that carnosic acid decreased the expression levels of TYR /MITF /TYRP1 /TYRP2 and melanin content compared to alpha-melanocite-stimulating hormone-treated B16F1 cells. The tyrosinase activity assay also revealed that carnosic acid decreased melanin production in B16F1 cells. Conclusion: These data indicate that carnosic acid increases the whitening effects on the B16F1 cells. Therefore, carnosic acid is a potential ingredient that can be used for skin whitening. Additionally, further research on the mechanisms of the action of carnosic acid should be conducted for the development of functional foods, cosmetics, and medicines.
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