멜라노마 세포(B16F10)에서의 개망초 추출물을 이용한 미백효과에 관한 연구
A Study on the Whitening Effect of Erigeron annuus (L.) Pers. Ethanol Extract on Melanoma Cell (B16F10)
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A 70% ethanol extract of Erigeron annuus (L.) Pers. was investigated for its whitening activity for application as a functional ingredient in cosmetic products. At the E. annuus extract concentration of 1,000 μg/ml, the electron-donating ability was found to be 67.83%, the tyrosinase inhibitory effect (related to skin-whitening) was 69%, the elastase inhibitory effect (related to skin-wrinkling) was 69%, and the astringent effect was 80%. The ABTS+ radical-scavenging ability was 87% at the 500 μg/ml concentration. In the cell viability test measured on melanoma cells, 96% of the cells treated with 100 μg/ml of the extract were viable. According to the western blot results, the protein expression of the microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein (TRP) -1, and TRP-2 was decreased by 60.22%, 47.83%, 54.79%, and 67.88%, respectively, at the extract concentration of 100 μg/ml. The protein expression of phosphorylated extracellular signal regulated kinase (p-ERK) and phosphorylated cAMP response elementbinding protein (p-CREB) was decreased with increasing concentrations of the extract. Reverse transcription- polymerase chain reaction of the extract showed that the mRNA expression of MITF, tyrosinase, TRP- 1, and TRP-2 was decreased by 86.51%, 85.22%, 74.26%, and 66.66%, respectively, at 100 μg/ml extract concentration. The findings suggest that the 70% ethanol extract from E. annuus (L.) Pers. has potential as a cosmeceutical ingredient with whitening effect.
Abstract
A 70% ethanol extract of Erigeron annuus (L.) Pers. was investigated for its whitening activity for application as a functional ingredient in cosmetic products. At the E. annuus extract concentration of 1,000 μg/ml, the electron-donating ability was found to be 67.83%, the tyrosinase inhibitory effect (related to skin-whitening) was 69%, the elastase inhibitory effect (related to skin-wrinkling) was 69%, and the astringent effect was 80%. The ABTS+ radical-scavenging ability was 87% at the 500 μg/ml concentration. In the cell viability test measured on melanoma cells, 96% of the cells treated with 100 μg/ml of the extract were viable. According to the western blot results, the protein expression of the microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein (TRP) -1, and TRP-2 was decreased by 60.22%, 47.83%, 54.79%, and 67.88%, respectively, at the extract concentration of 100 μg/ml. The protein expression of phosphorylated extracellular signal regulated kinase (p-ERK) and phosphorylated cAMP response elementbinding protein (p-CREB) was decreased with increasing concentrations of the extract. Reverse transcription- polymerase chain reaction of the extract showed that the mRNA expression of MITF, tyrosinase, TRP- 1, and TRP-2 was decreased by 86.51%, 85.22%, 74.26%, and 66.66%, respectively, at 100 μg/ml extract concentration. The findings suggest that the 70% ethanol extract from E. annuus (L.) Pers. has potential as a cosmeceutical ingredient with whitening effect.
참고문헌 (33)
- 김은진, "자생식물과 생약자원 추출물의 폴리페놀, 플라보노이드 함량 및 항산화 활성 탐색", 한국식품과학회지, 44(3), pp.337~342 (2012), 한국식품과학회[DOI]
- 김재원, "추출방법에 따른 돼지감자 잎의 항산화 및 생리활성 비교", 한국식품영양과학회지, 42(1), pp.68~75 (2013), 한국식품영양과학회
- 염태현, "측백나무 열매 추출물의 항균활성", 한국약용작물학회지, 18(5), pp.315~322 (2010), 한국약용작물학회
- Parvez S, "Survey and mechanism of skin depigmenting and lightening agent", Phytother Res, 20, pp.921~934 (2006)
- Seiberg M, "Inhibition of melanosome transfer results in skin lightening", J. Invest. Dermatol, 115, pp.162~167 (2000)
- Hunt G, "Alpha-melanocyte stimulating hormone and its analogue Nle4DPhe7 alpha-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes", J. Cell Sci, 107, pp.205~216 (1994)
- Busca R, "Cyclic AMP a key messenger in the regulation of skin pigmentation", Pigm. Cell Res, 13, pp.60~69 (2000)
- Fordin M, "Cyclic AMP activates the mitogen-activated protein kinase cascade in PC12 cells", J. Biol. Chem, 269, pp.6207~6214 (1994)
- Lee JH, "Effects of toxic substances of Erigeron annus on the germination and seeding growth of several upland crops", Wonkwang Univ, 17, pp.107~139 (1983)
- 김동현, "식용 식물자원으로부터 활성물질의 탐색 XIII. 개망초(Erigeron annuus L.) 꽃으로부터 triterpenoid의 분리", Applied Biological Chemistry, 47(4), pp.422~425 (2004), 한국응용생명화학회
- 신윤숙, "개망초 추출물을 이용한 천연염색 (제2보) - 면섬유에 대한 염색성 -", 한국의류학회지, 28(12), pp.1625~1631 (2004), 한국의류학회
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 26, pp.1199~1120 (1958)
- Roberta R, "Antioxidant acitivity applying an improved ABTS radical cation decolorization assay", Free Radic. Biol. Med, 26, pp.1231~1237 (1999)
- Yagi A, "The effect of tyrosinase inhibition for aloe", Planta Med, 3981, pp.517~519 (1986)
- Cannell RJP, "Results of a large scale screen of microalgae for the production of protease inhibitors", Planta. Med, 54, pp.10~14 (1988)
- 이진태, "함초의 생리활성기능과 화장품소재로서의 응용", 대한본초학회지, 17(2), pp.6~60 (2002), 대한본초학회
- Carmichael J, "Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing", Cancer Res, 47, pp.936~942 (1987)
- Hatano T, "Constituents of natural medicines with scavenging effects on active oxygen species tannins and related polyphenols", Nat. Med, 49, pp.357~363 (1995)
- Riley PA, "Molecules in focus melanin", Int. J. Biochem. Cell Biol, 29, pp.1235~1239 (1997)
- 주종찬, "한약재 열수추출물의 항산화 활성", 한국식품영양과학회지, 35(1), pp.7~14 (2006), 한국식품영양과학회
- 조경순, "피막이풀의 DPPH 라디칼과 hydroxyl radicals (OH) 항산화 활성 및 리폭시게나아제 저해 효과", 생명과학회지, 26(9), pp.1022~1026 (2016), 한국생명과학회
- Awika JM, "Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products", Agric. Food Chem, 51, pp.6657~6719 (2003)
- 김연숙, "돼지감자잎 추출물의 항산화 활성 및 간세포 보호 효과", 한국식품영양과학회지, 40(11), pp.1525~1531 (2011), 한국식품영양과학회
- 차재영, "한방 생약재 발효액의 항산화 활성 및 tyrosinase 저해 활성", 생명과학회지, 20(6), pp.940~947 (2010), 한국생명과학회
- Im S, "Activation of the cAMP pathway by α-melanotropin mediates the response of human melanocytes to ultraviolet B radiation", Cancer Res, 58, pp.47~54 (1998)
- Iwata M, "The relationship between tyrosinase activity and skin color in human foreskins", J. Invest. Dermatol, 95, pp.9~15 (1990)
- 이세영, "아가위(Crataegus pinnatifida Bunge)로 부터 HS 68세포의 MMP-1에 대한 저해활성 물질의 분리", Applied Biological Chemistry, 46(1), pp.60~65 (2003), 한국응용생명화학회
- Tsuji N, "The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity", Photochem. Photobiol, 74, pp.283~290 (2001)
- 임애경, "포공영의 열수 및 에탄올 추출물의 기능적 생리활성", 한국식품영양과학회지, 37(10), pp.1231~1237 (2008), 한국식품영양과학회
- 윤진숙, "눈개승마 추출물의 항산화 및 주름개선 효과", Food Science and Preservation, 19(3), pp.393~399 (2012), 한국식품저장유통학회[DOI]
- -bis(4-methoxy- 6-nitro) benzenesulfonic acid hydrate reduction by xanthinexanthine oxidase, "Anal. Biochem", 1997, 206~215
- 김보윤, "봄맞이 추출물의 미백 효능 연구", 대한화장품학회지, 41(1), pp.21~26 (2015), 사단법인 대한화장품학회[DOI]
- Hearing VJ, "Fuctional properties of cloned melanogenic proteins", Pigment Cell Res, 5, pp.264~334 (1992)