Copy Paper as a Platform for Low-cost Sensitive Glucose Sensing
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study reports the potential of using commercial copy papers as substrates for simple sensitive glucose detection. Typical paper-based devices use filter papers as porous substrates that can contain reagents; however, this is the first study to report the use of copy papers for the purpose of enhancing enzymatic colorimetric detection. Glucose detection using glucose oxidase, horseradish peroxidase and potassium iodide was performed on a copy paper, cellulose-based filter paper, and polyethylene film. The results indicated that the copy paper exhibited a stronger coloration than the other substrates. Reagents required for detection were dried on the copy paper, and a 3D-printed holder was designed to provide an environment for consistent imaging, making it a convenient cost-effective option for point-of-care testing using a mobile phone camera. The simple paper-based glucose sensor exhibited a linear range of 0.1–20 mM, limit of quantification of 0.477 mM, and limit of detection of 0.143 mM.
Abstract
This study reports the potential of using commercial copy papers as substrates for simple sensitive glucose detection. Typical paper-based devices use filter papers as porous substrates that can contain reagents; however, this is the first study to report the use of copy papers for the purpose of enhancing enzymatic colorimetric detection. Glucose detection using glucose oxidase, horseradish peroxidase and potassium iodide was performed on a copy paper, cellulose-based filter paper, and polyethylene film. The results indicated that the copy paper exhibited a stronger coloration than the other substrates. Reagents required for detection were dried on the copy paper, and a 3D-printed holder was designed to provide an environment for consistent imaging, making it a convenient cost-effective option for point-of-care testing using a mobile phone camera. The simple paper-based glucose sensor exhibited a linear range of 0.1–20 mM, limit of quantification of 0.477 mM, and limit of detection of 0.143 mM.
참고문헌 (17)
- A. W. Martinez, "Patterned paper as a platform for inexpensive, low-volume, portable bioassays", Angew. Chem. Int. Ed, 119(8), pp.1340~1342 (2007)
- Xiaolu Xiong, "Simultaneous Multiplexed Detection of Protein and Metal Ions by a Colorimetric Microfluidic Paper-based Analytical Device", BioChip Journal, 14(4), pp.429~437 (2020), 한국바이오칩학회[DOI]
- H. S. Siebe, "Filter paper based SERS substrate for the direct detection of analytes in complex matrices", Analyst, 146(4), pp.1281~1288 (2021)
- J. Su, "Engineering paper as a substrate for blood typing bio-diagnostics", Cellulose, 19(5), pp.1749~1758 (2012)
- E. Bihar, "A fully inkjet-printed disposable glucose sensor on paper", npj Flex. Electron, 2(1), pp.1~8 (2018)
- Y. Lu, "Fabrication and characterization of paper-based microfluidics prepared in nitrocellulose membrane by wax printing", Anal. Chem, 82(1), pp.329~335 (2010)
- E. Carrilho, "Understanding wax printing : a simple micropatterning process for paper-based microfluidics", Anal. Chem, 81(16), pp.7091~7095 (2009)
- C. -M. Cheng, "Millimeter-scale contact printing of aqueous solutions using a stamp made out of paper and tape", Lab Chip, 10(23), pp.3201~3205 (2010)
- 전형진, "Paper-based Glucose Biosensing System Utilizing a Smartphone as a Signal Reader", BioChip Journal, 8(3), pp.218~226 (2014), 한국바이오칩학회[DOI]
- P. de Tarso Garcia, "A handheld stamping process to fabricate microfluidic paper-based analytical devices with chemically modified surface for clinical assays", Rsc Adv, 4(71), pp.37637~37644 (2014)
- X. Sun, "Rotational paper-based electrochemiluminescence immunodevices for sensitive and multiplexed detection of cancer biomarkers", Anal. Chim. Acta, 1007, pp.33~39 (2018)
- N. R. Pollock, "A paper-based multiplexed transaminase test for low-cost, point-of-care liver function testing", Sci Transl Med, 4(152), pp.152ra129~152ra129 (2012)
- T. Soga, "Inkjetprinted paper-based colorimetric sensor array for the discrimination of volatile primary amines", Anal. Chem, 85(19), pp.8973~8978 (2013)
- K. Yamada, "Dip-and-read”paper-based analytical devices using distance-based detection with color screening", Lab Chip, 18(10), pp.1485~1493 (2018)
- X. Luo, "Cellulosebased strips designed based on a sensitive enzyme colorimetric assay for the low concentration of glucose detection", Anal. Chem, 91(24), pp.15461~15468 (2019)
- N. M. Neris, "3D Multilayered paper-and thread", paper-based microfluidic devices for bioassays, 2019, pp.40(2), 296~303
- V. Kishnani, "A ChemometricAssisted Colorimetric-Based Inexpensive Paper Biosensor for Glucose Detection", Biosensors, 12(11), pp.1008(1)~1008(16) (2022)