Direct Detection of Water-dissolved Ammonia Using Paper-based Analytical Devices
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
A microfluidic paper-based analytical device (µPAD) is proposed for the selective detection of ammonia in water by using the modified Berthelot reagent and a fluidic channel consisting of hollow paper. The modified Berthelot reagents were uniformly dispersed in cyclohexane and then immobilized in a detection zone of the µPAD. The loading position of the reagents and the type of a sample flow channel were optimized to achieve a sensitive ammonia detection within a short analytical time. The NH3 µPAD exhibits a linear colorimetric response to the concentration of ammonia dissolved in water in the range of 1-100 mgL-1, and its limit-of-detection is 1.75mgL-1. In addition, the colorimetric response was not influenced by the addition of 100 mg L-1 nitrogen containing compounds (sodium nitrate, sodium nitrite, uric acid, hydroxylamine, butylamine, diethylamine) or inorganic salts (NaCl, Na2HPO4), presenting the enough selectivity in the detection of water-dissolved ammonia against possible interferents.
Abstract
A microfluidic paper-based analytical device (µPAD) is proposed for the selective detection of ammonia in water by using the modified Berthelot reagent and a fluidic channel consisting of hollow paper. The modified Berthelot reagents were uniformly dispersed in cyclohexane and then immobilized in a detection zone of the µPAD. The loading position of the reagents and the type of a sample flow channel were optimized to achieve a sensitive ammonia detection within a short analytical time. The NH3 µPAD exhibits a linear colorimetric response to the concentration of ammonia dissolved in water in the range of 1-100 mgL-1, and its limit-of-detection is 1.75mgL-1. In addition, the colorimetric response was not influenced by the addition of 100 mg L-1 nitrogen containing compounds (sodium nitrate, sodium nitrite, uric acid, hydroxylamine, butylamine, diethylamine) or inorganic salts (NaCl, Na2HPO4), presenting the enough selectivity in the detection of water-dissolved ammonia against possible interferents.
참고문헌 (19)
- R. Braun, "Ammonia toxicity in liquid piggery manure digestion", Biotechnol. Lett, 3(4), pp.159~164 (1981)
- V. Walker, "Ammonia toxicity and its prevention in inherited defects of the urea cycle", Diabetes Obes. Metab, 11(9), pp.823~835 (2009)
- R. V. Thurston, "Ammonia toxicity to fishes. Effect of pH on the toxicity of the unionized ammonia species", Enrion. Sci. Technol., 15(7), pp.837~840 (1981)
- J. E. Ryer-Powder, "Health Effects of Ammonia", Plant, 1991, pp.10(4), 228~232
- N. F. Y. Tam, "Effect of ammonia concentrations on growth of Chlorella vulgaris and nitrogen removal from media", Biosesour. Technol, 57(1), pp.45~50 (1996)
- S. Alegret, "Flow Injection System for On-line Potentiometric Monitoring of Ammonia in Freshwater Streams", Analyst, 114(11), pp.1443~1447 (1989)
- A. Amirjani, "Colorimetric detection of ammonia using smartphones based on localized surface plasmon resonance of silver nanoparticles", Talanta, 176, pp.242~246 (2018)
- F. J. Krug, "Determination of Ammonia in Low Concentrations with Nessler’s Reagent by Flow Injection Analysis", Analyst, 104(1234), pp.47~54 (1979)
- R. T. Emmet, "Spectrophotometric determination of urea and ammonia in natural waters with hypochlorite and phenol", Anal. Chem, 41(12), pp.1648~1652 (1969)
- M. D. Krom, "Spectrometric determination of ammonia: a study of a modified Berthelot reaction using salicylate and dichloroisocyanurate", Analyst, 105(1249), pp.305~316 (1980)
- H. Verdouw, "Ammonia determination based on indophenol formation with sodium salicylate", Water Res, 12(6), pp.399~402 (1978)
- C. E. Bower, "A salicylate-Hypochlorite Method for Determining Ammonia in Seawater", Can. J. Fish. Aquat, 37(5), pp.794~798 (1980)
- M. M. Mentele, "Microfluidic paper-based analytical device for particulate metals", Anal. Chem., 84(10), pp.4474~4480 (2012)
- A. W. Martinez, "Patterned paper as a platform for inexpensive, lowvolume, portable bioassays", Angew. Chem. Int. Ed, 46(8), pp.1318~1320 (2007)
- N. L. Nxumalo, "Development of a paper-based microfluidic device for the quantification of ammonia in industrial wastewater", Water SA, 46(3), pp.506~513 (2020)
- T. Thongkam, "A simple paper-based analytical device using UV resin screenprinting for the determination of ammonium in soil", Anal. Methods, 12(38), pp.4649~4656 (2020)
- B. M. Jayawardane, "Development of a gas-diffusion microfluidic paper-based analytical device(μPAD)for the determination of ammonia in wastewater samples", Anal. Chem, 87(9), pp.4621~4626 (2015)
- K. T. Lau, "Solid-state ammonia sensor based on Berthelot’s reaction", Sens. Actuators B Chem, 98(1), pp.12~17 (2004)
- R. Sander, "Compilation of Henry’s law constants(version 4. 0)for water as solvent", Atmos. Chem. Phys, 15(8), pp.4399~4981 (2015)