Development of Single-layer-structured Glucose Biosensor

Abstract

In this paper, we fabricated a low-cost glucose sensor with a simpler structure and fabrication process than the existing glucose sensor. The currently used glucose sensor has a three-layer structure with upper, middle, and bottom plates; here, we fabricated a single-layerglucose sensor using only a printing and dispensing process. We successfully fabricated the glucose sensor using a simple methodinvolving the formation of an electrode and insulator layer through a 2- or 3-step printing process on plastic or paper film, followed bythe dispensing of glucose oxidase solution on the electrode. Cyclic voltammetry (CV) and cyclic amperometry (CA) measurements wereused to evaluate the characteristics of the fabricated single-layer glucose sensor. Also, its sensitivity was analyzed through glucose-controlledblood measurements. Hence, a low-cost single-layer glucose sensor was fabricated with evaluation of its characteristics demonstratingthat it has useful application in medicine.