율피 추출물이 3T3-L1 지방전구세포 분화에 미치는 영향
Effects of Chestnut Inner Shell Extract on 3T3-L1 Preadipocyte Differentiation
2
조회
0
다운로드
8
인용
0
참고문헌
키워드
초록
Obesity occurred by energy imbalance, is increasing regardless of race, sex, age, and related to the metabolic syndrome, diabetes and cardiovascular disease. Since adipose tissue plays a critical role in regulating energy homeostasis, understanding of adipogenesis pathway and finding of regulatory mechanism for adipogenesis can be helpful to manage obesity as well as obesity-related diseases. In this study, to investigate the effects of Chestnut Inner Shell(CIS) extract on the adipogenesis in 3T3-L1 preadipocytes, 3T3-L1 preadipocytes were differentiated with adipogenic reagents for 9 days in the absence or presence of CIS extract ranging from 10 - 100 ㎍/㎖. The effect of CIS extract on 3T3-L1 differentiation was examined by measuring intracelluar lipid droplet and triglyceride contents. CIS extract remarkably inhibited lipid accumulation(about 45% inhibition at 100 ㎍/㎖ of CIS extract) and slightly decreased triglyceride contents(about 15% decrease at 100 ㎍/㎖ of CIS extract) in 3T3-L1 preadipocytes at the concentration showing no cytotoxicity. These results demonstrated that CIS extract significantly inhibit adipogenesis and can be used for the regulation of obesity.
Abstract
Obesity occurred by energy imbalance, is increasing regardless of race, sex, age, and related to the metabolic syndrome, diabetes and cardiovascular disease. Since adipose tissue plays a critical role in regulating energy homeostasis, understanding of adipogenesis pathway and finding of regulatory mechanism for adipogenesis can be helpful to manage obesity as well as obesity-related diseases. In this study, to investigate the effects of Chestnut Inner Shell(CIS) extract on the adipogenesis in 3T3-L1 preadipocytes, 3T3-L1 preadipocytes were differentiated with adipogenic reagents for 9 days in the absence or presence of CIS extract ranging from 10 - 100 ㎍/㎖. The effect of CIS extract on 3T3-L1 differentiation was examined by measuring intracelluar lipid droplet and triglyceride contents. CIS extract remarkably inhibited lipid accumulation(about 45% inhibition at 100 ㎍/㎖ of CIS extract) and slightly decreased triglyceride contents(about 15% decrease at 100 ㎍/㎖ of CIS extract) in 3T3-L1 preadipocytes at the concentration showing no cytotoxicity. These results demonstrated that CIS extract significantly inhibit adipogenesis and can be used for the regulation of obesity.
참고문헌 (38)
- Ordovas, J.M, "Nutrigenomic and nutrigenetics", Curr Opin Lipidol, 15, pp.101~108 (2004)
- Flegal, K.M, "Prevalence and trends in obesity among US adults, 1999-2000", JAMA, 288(14), pp.1723~1727 (2000)
- Kereiakes, D.J, "Metabolic syndrome epidemic", Circulation, 108(13), pp.1552~1553 (2003)
- Mokdad, A.H, "Prevalence of obesity, diabetes and obesity related health risk factors, 2001", JAMA, 289(1), pp.76~79 (2001)
- Jee, S.H, "Body-mass index and mortality in Korean men and women", N Engl J Med, 355(8), pp.779~787 (2006)
- 배남규, "한국인의 10년간 비만수준의 변화 양상: 1997~2007", 대한비만학회지, 18(1), pp.24~30 (2009), 대한비만학회
- 조연지, "3T3-L1 아지방세포의 분화에 미치는 anthocyanins의 영향", 석사, pp.2006
- Yang, J.Y, "Effect of acanthopanax senticosus on lipoprotein lipase in 3T3-L1 adipocytes", Phytother Res, 18, pp.160~163 (2004)
- Sanders, S.R, "Effects of specific conjugated linoleic acid isomers on growth characteristics in obese Zucker rats", Lipids, 39, pp.537~543 (2004)
- Ahima, R.S, "Adipose tissue as an endocrine organ", Trend Endocrinol Metab, 11(8), pp.327~332 (2000)
- 김만조, "밤나무 재배 품종의 개화, 결실 및 과실특성", 한국임학회지, 92(4), pp.321~332 (2003), 한국임학회
- 손철호, "국내 밤산업의 동향과 전망", pp.77~79 (2003), 농촌경제연구소
- Choi, O.B, "Anti-allergic effect of Castanea crenata leaf tea", Korean J Food Sci Technol, 30, pp.468~471 (1998)
- Kwon, E.J, "Antioxidative activity of solvent fraction and isolation of antioxidant compound from chestnut husk", J Korean Soc Food Sci Nutr, 30, pp.726~731 (2001)
- Kim, S.K, "Sensory evaluation and retrogradation properties of chestnut mook", J Korean Soc Food Nutr, 24, pp.601~605 (1995)
- Park, Y.H, "Rheological properties of gelatinized chestnut starch solution", Korean J Food Sci Technol, 16, pp.314~318 (1984)
- Cho, S.H, "Effect of blanching on the prevention of discoloration in the thermal-treated chestnut power", J Korean Soc Food Nutr, 17, pp.211~214 (1988)
- Yoon, W.J, "The study on the humidity preserving effect with several natural packs" (1997), Dong Duck Women`s University
- 오승희, "율피, 솔잎, 호프의 아세톤 추출물의 항산화 작용", 한국식생활문화학회지, 19(4), pp.399~406 (2004), 한국식생활문화학회
- 이진태, "율피의 생리활성 및 미백효과를 이용한 화장품신소재에 관한 연구", 대한본초학회지, 20(2), pp.27~33 (2005), 대한본초학회
- 이현주, "국내산 밤 일부 품종의 기능성 성분분석과 항산화 및 대식세포 활성", 한국식품영양과학회지, 37(9), pp.1095~1100 (2008), 한국식품영양과학회
- Hsu, C.L, "Effect of gallic acid on high fat diet-induced dyslipidaemia, hepatosteatosis and oxidative stress in rats", British journal of Nutrition, 98, pp.727~735 (2007)
- 홍정희, "고지방 식이로 유도된 비만쥐에서 녹차 Catechin이 체지방 조성 및 지방조직에 미치는 영향", 한국식품영양과학회지, 36(5), pp.540~547 (2007), 한국식품영양과학회
- 김미자, "Caffeine이 지방세포주 3T3-L1 분화에 미치는 영향：영양유전체학적 접근", 한국영양학회지, 38(8), pp.649~655 (2005), 한국영양학회
- 허 준, "동의보감", pp.3449~ (2005), 법인문화사
- Noh, J.R, "Antioxidant Effects of Chestnut Inner Shell Extract on High Fat-diet fed C57BL", 6 Mice and t-BHP treat HepG2 cells, 2009, 206~
- Noh, J.R, "Hypolipidemic Effects of Chestnut Inner Shell Extract on High Fat-diet fed C57BL", 6 Mice, 2009, 205~
- 양민진, "율피·솔잎·호프의 수성 아세톤 추출물에 의한 Melanin 생성 억제 효과", 약학회지, 43(4), pp.494~501 (1999)
- 진동언, "율피 추출물이 티로시나아제 유전자 발현에 미치는 효과", 대한위생학회지, 20(3), pp.10~16 (2005)
- 진휘승, "항비만에 대한 생약복합물의 효과", 한국식품영양과학회지, 38(1), pp.32~38 (2009), 한국식품영양과학회
- Ailhaud, G, "Cellular and molecular aspects of adipose tissue development", Annu Rev Nutr, 12, pp.207~233 (1992)
- Haugen, F, "Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid", Lipid Res, 46, pp.143~153 (2005)
- Tenney, R, "Interleukin 11 signaling in 3T3-L1 adipocytes", J cell physiol, 202, pp.160~166 (2005)
- Guo, X, "Analysis gene expression profile during 3T3-L1 preadipocytes differentiation", Gene, 251, pp.45~53 (2000)
- MacDougald, O.A, "Regulated expression of the obese gene product(leptin) in white adipose tissue and 3T3-L1 adipocytes", Proc Natl Acad Sci USA, 92(20), pp.9034~9037 (1995)
- Klaus, S, "Adipose tissue as a regulator of energy balance", Curr Drug Targets, 5(3), pp.241~250 (2004)
- Kim, J.B, "ADD1", SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism, 1996, pp.10, 1096~1107
- Koldovskỳ, O, "Developmental aspects of lipid metabolism", Physiol Res, 44(6), pp.353~356 (1995)