3T3-L1 세포에서 Adipogenesis 억제를 통한 Kaki-tannin의 항비만 활성
Anti-obese Activities of Kaki-tannins Prepared from Various Persimmons through Inhibition of 3T3-L1 Adipogenesis
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Persimmon (Diospyros kaki Thunb.) is widely cultivated in Asian countries such as Korea, China, andJapan. Although its various beneficial functions have been well-documented, its anti-obese effect and isrelatively unknown. Here, we prepared kaki-tannins from unripe astringent Daebong persimmon (DU), redripenon-astringent Daebong persimmon (DR), unripe astringent Cheongdo flat shaped persimmon (CU),red-ripe non-astringent Cheongdo flat shaped persimmon (CR), and unripe non-astringent Fuyu persimmon(FU). Cytotoxicity of the kaki-tannins were evaluated by MTT assay in 3T3-L1 cells. The treatment up to100 μg/mL of all kaki-tannins for 24 h did not affect to the 3T3-L1 cell viability. Thus, the concentration upto 100 μg/mL of the kaki-tannins were chosen for subsequent experiments. The treatment with 100 μg/mLof kaki-tannins prepared from DR and CU significantly inhibited lipid accumulation of 3T3-L1 adipocytes. In line with this result, the decreased number and size of intracellular lipid droplets in 3T3-L1 adipocytestreated with DR and CU kaki-tannins were observed. The 3T3-L1 cells treated with DR and CU kaki-tanninsshowed down-regulated mRNA expression levels of C/EBPα and FAS as judged by RT-PCR. Our studyalso revealed that DR and CU kaki-tannins-inhibited adipogenesis are strongly limited in the early stageof 3T3-L1 adipogenesis. These evidences suggest that kaki-tannin has the potential as anti-obese naturalmaterials, and different function and role of kaki-tannins in anti-adipogenic activities may be mediated topersimmon types and maturation stage.
Abstract
Persimmon (Diospyros kaki Thunb.) is widely cultivated in Asian countries such as Korea, China, andJapan. Although its various beneficial functions have been well-documented, its anti-obese effect and isrelatively unknown. Here, we prepared kaki-tannins from unripe astringent Daebong persimmon (DU), redripenon-astringent Daebong persimmon (DR), unripe astringent Cheongdo flat shaped persimmon (CU),red-ripe non-astringent Cheongdo flat shaped persimmon (CR), and unripe non-astringent Fuyu persimmon(FU). Cytotoxicity of the kaki-tannins were evaluated by MTT assay in 3T3-L1 cells. The treatment up to100 μg/mL of all kaki-tannins for 24 h did not affect to the 3T3-L1 cell viability. Thus, the concentration upto 100 μg/mL of the kaki-tannins were chosen for subsequent experiments. The treatment with 100 μg/mLof kaki-tannins prepared from DR and CU significantly inhibited lipid accumulation of 3T3-L1 adipocytes. In line with this result, the decreased number and size of intracellular lipid droplets in 3T3-L1 adipocytestreated with DR and CU kaki-tannins were observed. The 3T3-L1 cells treated with DR and CU kaki-tanninsshowed down-regulated mRNA expression levels of C/EBPα and FAS as judged by RT-PCR. Our studyalso revealed that DR and CU kaki-tannins-inhibited adipogenesis are strongly limited in the early stageof 3T3-L1 adipogenesis. These evidences suggest that kaki-tannin has the potential as anti-obese naturalmaterials, and different function and role of kaki-tannins in anti-adipogenic activities may be mediated topersimmon types and maturation stage.
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