3T3-L1 세포 모델에서 Adipogenesis 조절을 통한 녹색입 홍합 추출물의 항비만 활성
Anti-obese Activity of Water Extract Prepared from Green-shelled Mussel through Regulating Adipogenesis
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Adipogenesis was defined as a differentiation process of preadipocytes into adipocytes. It is a key processin determining the number of mature adipocytes in the development of obesity. Thus, to regulation ofadipogenesis is crucial target in obesity prevention. Mussel (Mytilus corscus ) has been reported to possessabundant dietary proteins and savory amino acids. However, antiadipogenic role and function in obesityprevention remains unknown. Here, we employed green-shelled mussel and its water extract (GME) wasprepared. To elucidate the potential role of GME in 3T3-L1 adipogenesis, 3T3-L1 cells were treated with GMEbetween day –2 to day 6. We found that GME dose-dependently suppressed adipogenesis and inhibitedformation of lipid droplets accompanied by reduced level of adipogenic transcriptional factors such as C/EBPαand FAS. Our study further revealed that inhibitory function of GME wa largely limited to the adipogenesis day0 to day 6. Taken together, our evidences provide new insights into the molecular basis underlying the antiadipogenicfunction of GME.
Abstract
Adipogenesis was defined as a differentiation process of preadipocytes into adipocytes. It is a key processin determining the number of mature adipocytes in the development of obesity. Thus, to regulation ofadipogenesis is crucial target in obesity prevention. Mussel (Mytilus corscus ) has been reported to possessabundant dietary proteins and savory amino acids. However, antiadipogenic role and function in obesityprevention remains unknown. Here, we employed green-shelled mussel and its water extract (GME) wasprepared. To elucidate the potential role of GME in 3T3-L1 adipogenesis, 3T3-L1 cells were treated with GMEbetween day –2 to day 6. We found that GME dose-dependently suppressed adipogenesis and inhibitedformation of lipid droplets accompanied by reduced level of adipogenic transcriptional factors such as C/EBPαand FAS. Our study further revealed that inhibitory function of GME wa largely limited to the adipogenesis day0 to day 6. Taken together, our evidences provide new insights into the molecular basis underlying the antiadipogenicfunction of GME.
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