3T3-L1 지방전구세포에서 1-O-feruloyl-β-D-glucose의 지방생성억제효과
Anti-adipogenic effect of 1-O-feruloyl-β-D-glucose on 3T3-L1 preadipocytes
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Obesity is the most common metabolic disease and is caused by an excess accumulation of body fat. 1-O-Feruloyl-β-D-glucose (FG) is a phenolic compound isolated from the corks of Euonymus alatus (Thunb.) Sieb. This study was designed to investigate the effect of FG on adipogenesis using 3T3-L1 preadipocyte. First, we evaluated the effects of FG on cell proliferation and cytotoxicity. Up to 5 μM, FG did not affect the proliferation of or cause cytotoxicity in 3T3-L1 preadipocytes. Therefore, we used FG at 0-5 μM for subsequent experiments. FG inhibited the accumulation of lipid droplets in differentiated 3T3-L1 adipocytes in a concentration-dependent manner. To understand the mechanism underlying the anti-adipogenic effect of FG, western blot analyses were performed. We found that FG suppressed peroxisome proliferator-activated receptor (PPAR) γ protein expression, a representative adipogenesis-related transcription factor. In addition, FG inhibited aP2/fatty acid binding protein 4 (FABP4) protein expression, a target gene of PPARγ. In conclusion, these data suggest that FG inhibits adipogenesis in 3T3-L1 adipocytes by inhibiting the expression of PPARγ and FABP4.
Abstract
Obesity is the most common metabolic disease and is caused by an excess accumulation of body fat. 1-O-Feruloyl-β-D-glucose (FG) is a phenolic compound isolated from the corks of Euonymus alatus (Thunb.) Sieb. This study was designed to investigate the effect of FG on adipogenesis using 3T3-L1 preadipocyte. First, we evaluated the effects of FG on cell proliferation and cytotoxicity. Up to 5 μM, FG did not affect the proliferation of or cause cytotoxicity in 3T3-L1 preadipocytes. Therefore, we used FG at 0-5 μM for subsequent experiments. FG inhibited the accumulation of lipid droplets in differentiated 3T3-L1 adipocytes in a concentration-dependent manner. To understand the mechanism underlying the anti-adipogenic effect of FG, western blot analyses were performed. We found that FG suppressed peroxisome proliferator-activated receptor (PPAR) γ protein expression, a representative adipogenesis-related transcription factor. In addition, FG inhibited aP2/fatty acid binding protein 4 (FABP4) protein expression, a target gene of PPARγ. In conclusion, these data suggest that FG inhibits adipogenesis in 3T3-L1 adipocytes by inhibiting the expression of PPARγ and FABP4.
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