Anti-adipogenic effect of mulberry leaf ethanol extract in 3T3-L1 adipocytes
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BACKGROUND/OBJECTIVES: Adipogenesis is part of the cell differentiation process in which undifferentiated fibroblasts(pre-adipocytes) become mature adipocytes with the accumulation of lipid droplets and subsequent cell morphological changes. Several transcription factors and food components have been suggested to be involved in adipogenesis. The aim of this studywas to determine whether mulberry leaf ethanol extract (MLEE) affects adipogenesis in 3T3-L1 adipocytes. MATERIALS/METHODS: The 3T3-L1 adipocytes were treated with different doses of MLEE for 8 days starting 2 days post-confluence. Cell viability, fat accumulation, and adipogenesis-related factors including CCAAT-enhancer-binding protein alpha (C/EBPα),peroxisome proliferator-activated receptor gamma (PPARγ), PPARγ coactivator 1 alpha (PGC-1α), fatty acid synthase (FAS), andadiponectin were analyzed. RESULTS: Results showed that MLEE treatments at 10, 25, 50, and 100 μg/ml had no effect on cell morphology and viability. Without evident toxicity, all MLEE treated cells had lower fat accumulation compared with control as shown by lower absorbancesof Oil Red O stain. MLEE at 50 and 100 μg/ml significantly reduced protein levels of PPARγ, PGC-1α, FAS, and adiponectinin differentiated adipocytes. Furthermore, protein level of C/EBPα was significantly decreased by the treatment of 100 μg/mlMLEE. CONCLUSION: These results demonstrate that MLEE treatment has an anti-adipogenic effect in differentiated adipocytes withouttoxicity, suggesting its potential as an anti-obesity therapeutic.
Abstract
BACKGROUND/OBJECTIVES: Adipogenesis is part of the cell differentiation process in which undifferentiated fibroblasts(pre-adipocytes) become mature adipocytes with the accumulation of lipid droplets and subsequent cell morphological changes. Several transcription factors and food components have been suggested to be involved in adipogenesis. The aim of this studywas to determine whether mulberry leaf ethanol extract (MLEE) affects adipogenesis in 3T3-L1 adipocytes. MATERIALS/METHODS: The 3T3-L1 adipocytes were treated with different doses of MLEE for 8 days starting 2 days post-confluence. Cell viability, fat accumulation, and adipogenesis-related factors including CCAAT-enhancer-binding protein alpha (C/EBPα),peroxisome proliferator-activated receptor gamma (PPARγ), PPARγ coactivator 1 alpha (PGC-1α), fatty acid synthase (FAS), andadiponectin were analyzed. RESULTS: Results showed that MLEE treatments at 10, 25, 50, and 100 μg/ml had no effect on cell morphology and viability. Without evident toxicity, all MLEE treated cells had lower fat accumulation compared with control as shown by lower absorbancesof Oil Red O stain. MLEE at 50 and 100 μg/ml significantly reduced protein levels of PPARγ, PGC-1α, FAS, and adiponectinin differentiated adipocytes. Furthermore, protein level of C/EBPα was significantly decreased by the treatment of 100 μg/mlMLEE. CONCLUSION: These results demonstrate that MLEE treatment has an anti-adipogenic effect in differentiated adipocytes withouttoxicity, suggesting its potential as an anti-obesity therapeutic.
참고문헌 (34)
- Ali AT, "Adipocyte and adipogenesis", Eur J Cell Biol, 92, pp.229~236 (2013)
- Lefterova MI, "New developments in adipogenesis", Trends Endocrinol Metab, 20, pp.107~114 (2009)
- Skurk T, "Relationship between adipocyte size and adipokine expression and secretion", J Clin Endocrinol Metab, 92, pp.1023~1033 (2007)
- Fu Y, "Adiponectin promotes adipocyte differentiation, insulin sensitivity, and lipid accumulation", J Lipid Res, 46, pp.1369~1379 (2005)
- Lehrke M, "The many faces of PPARgamma", Cell, 123, pp.993~999 (2005)
- Lefterova MI, "PPARgamma and C", EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scale, 2008, pp.22, 2941~2952
- Moldes M, "Peroxisome-proliferator-activated receptor gamma suppresses Wnt", beta-catenin signalling during adipogenesis, 2003, pp.376, 607~613
- Ross SE, "Inhibition of adipogenesis by Wnt signaling", Science, 289, pp.950~953 (2000)
- Liu J, "Functional interaction between peroxisome proliferator-activated receptor gamma and beta-catenin", Mol Cell Biol, 26, pp.5827~5837 (2006)
- Choi EM, "Effects of Morus alba leaf extract on the production of nitric oxide, prostaglandin E2 and cytokines in RAW264.7 macrophages", Fitoterapia, 76, pp.608~613 (2005)
- Lim HH, "Anti-inflammatory and antiobesity effects of mulberry leaf and fruit extract on high fat diet-induced obesity", EXPERIMENTAL BIOLOGY AND MEDICINE, 238, pp.1160~1169 (2013), SOC EXPERIMENTAL BIOLOGY MEDICINE
- 임현화, "Combined Treatment of Mulberry Leaf and Fruit Extract Ameliorates Obesity-Related Inflammation and Oxidative Stress in High Fat Diet-Induced Obese Mice", Journal of Medicinal Food, 16(8), pp.673~680 (2013), 한국식품영양과학회[DOI]
- Naowaboot J, "Mulberry leaf extract stimulates glucose uptake and GLUT4 translocation in rat adipocytes", Am J Chin Med, 40, pp.163~175 (2012)
- Tanabe K, "Repeated ingestion of the leaf extract from Morus alba reduces insulin resistance in KK-Ay mice", Nutr Res, 31, pp.848~854 (2011)
- Sugimoto M, "Mulberry leaf ameliorates the expression profile of adipocytokines by inhibiting oxidative stress in white adipose tissue in db", db mice, 2009, pp.204, 388~394
- Lee YJ, "Hypotensive, Hypolipidemic, and Vascular Protective Effects of Morus alba L. in Rats Fed an Atherogenic Diet", AMERICAN JOURNAL OF CHINESE MEDICINE, 39(1), pp.39~52 (2011), WORLD SCIENTIFIC PUBL CO PTE LTD
- Oh KS, "Melaninconcentrating hormone-1 receptor antagonism and anti-obesity effects of ethanolic extract from Morus alba leaves in diet-induced obese mice", J Ethnopharmacol, 122, pp.216~220 (2009)
- Peng CH, "Mulberry water extracts possess an anti-obesity effect and ability to inhibit hepatic lipogenesis and promote lipolysis", J Agric Food Chem, 59, pp.2663~2671 (2011)
- Cowherd RM, "Molecular regulation of adipocyte differentiation", Semin Cell Dev Biol, 10, pp.3~10 (1999)
- Puigserver P, "Tissue-specific regulation of metabolic pathways through the transcriptional coactivator PGC1-alpha", Int J Obes (Lond), 29(Suppl 1), pp.S5~S9 (2005)
- Kim MY, "The anti-angiogenic herbal composition Ob-X inhibits adipose tissue growth in obese mice", INTERNATIONAL JOURNAL OF OBESITY, 34, pp.820~830 (2010), NATURE PUBLISHING GROUP
- Rayalam S, "Resveratrol induces apoptosis and inhibits adipogenesis in 3T3-L1 adipocytes", Phytother Res, 22, pp.1367~1371 (2008)
- Tsuduki T, "Intake of 1-deoxynojirimycin suppresses lipid accumulation through activation of the beta-oxidation system in rat liver", J Agric Food Chem, 57, pp.11024~11029 (2009)
- Naowaboot J, "Kukongviriyapan U. Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes", Nutr Res, 32, pp.39~44 (2012)
- Tang QQ, "Sequential phosphorylation of CCAAT enhancer-binding protein beta by MAPK and glycogen synthase kinase 3beta is required for adipogenesis", Proc Natl Acad Sci U S A, 102, pp.9766~9771 (2005)
- Hamm JK, "A role for C", EBPbeta in regulating peroxisome proliferator-activated receptor gamma activity during adipogenesis in 3T3-L1 preadipocytes, 2001, pp.276, 18464~18671
- Darlington GJ, "The role of C", EBP genes in adipocyte differentiation, 1998, pp.273, 30057~30060
- Rosen ED, "PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro", Mol Cell, 4, pp.611~617 (1999)
- Wu Z, "Cross-regulation of C", EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and insulin sensitivity, 1999, pp.3, 151~158
- Trujillo ME, "Adiponectin--journey from an adipocyte secretory protein to biomarker of the metabolic syndrome", J Intern Med, 257, pp.167~175 (2005)
- Kadowaki T, "Adiponectin and adiponectin receptors", Endocr Rev, 26, pp.439~451 (2005)
- Pajvani UB, "Adiponectin: systemic contributor to insulin sensitivity", Curr Diab Rep, 3, pp.207~213 (2003)
- Tsuguhito Ota, "Chemokine Systems Link Obesity to Insulin Resistance", Diabetes and Metabolism Journal, 37(3), pp.165~172 (2013), 대한당뇨병학회
- Körner A, "Adiponectin expression in humans is dependent on differentiation of adipocytes and down-regulated by humoral serum components of high molecular weight", Biochem Biophys Res Commun, 337, pp.540~550 (2005)