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아시아에서 분리된 viral haemorrhagic septicaemia virus (VHSV) isolates의 계통분석학적 비교

Phylogenetic Analysis of Viral Haemorrhagic Septicaemia Virus (VHSV) Isolates from Asia

조미영|박명애|지보영|안상중
수산생명의학회 (Aquatic Life Medical Science)|2013.12|한국어병학회지 (JOURNAL OF FISH PATHOLOGY)|제26권 제3호|pp. 149-161|DOI http://dx.doi.org/10.7847/jfp.2013.26.3.149

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참고문헌

키워드

GlycoproteinOlive flounderParalichthys olivaceusViral haemorrhagic septicaemia virus (VHSV)

초록

바이러스성 출혈성 패혈증을 일으키는 VHSV는 국내의 넙치양식에 심각한 피해를 주고 있다. 본 연구에서는 우리나라에서 분리한 8종의 VHSV 분리주들의 glycoprotein (G)의 염기서열을 분석하여 Korean VHSV 분리주들과 기보고된 일본 및 중국의 분리주들을 계통발생학적으로 비교하여 G-protein의 8 부위 (G34, C528, A755, T834, T951, T1147, T1221, T1336)에서 국내 VHSV 분리주들의 특이적인 서열을 확인 하였고, 전 세계적으로 분리된 VHSV의 분리주들과의 비교결과 8 부위 중 3 부위 (A755, T834, T1221)의 국내VHSV 분리주 특이적 염기서열들을 확인하였다. 또한 중국 유래 VHSV 분리주의 특이적 염기서열 11 부위 (C498, C545, C548, C642, G648, T678, A738, C804, C834, G851, C1139)와 일본 유래 VHSV 분리주의 특이적 염기서열 1부위 (C179)를 확인하였다. 이상의 연구결과는 국내 특이적인 염기서열을 확보하여, 구역화된 수산생물 질병의 예찰, 모니터링 및 질병 관리에 유용하게 사용될 것으로 사료된다.

Abstract

Viral haemorrhagic septicaemia virus (VHSV), the causative agent of viral haemorrhagic septicaemia (VHS), is an epidemic virus of cultured olive flounder Paralichthys olivaceus in Korea. In the present study, the entire glycoprotein (G) gene including several hypervariable regions from 36 isolates of diverse geographic and host origin and 8 Korean VHSV isolates from cultured olive flounder were analyzed. Phylogenetic analysis indicated that most of Asian VHSV belong to the genotype IVa group, suggesting that they originated from a common ancestral virus. Comparative sequence analysis of the complete G protein from Korean VHSV isolates revealed 3 Korean strain-specific nucleotide residues (nucleotide number of G-region: A755, T834 and T1221). These results suggest that Korean VHSV originated from a common ancestor, but these regional specific nucleotide sequences suggest that genetic differences of VHSV are more related to geographic areas than to host fish species.

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관련 논문AI추천

Genetic positioning of Korean viral hemorrhagic septicemia virus (VHSV) from cultured and wild marine fishes김두운, 김위식, 김정호, 김종오, 오명주, 정성주수산생명의학회 · 한국어병학회지2011 · 제24권 제1호우리 나라 양식 넙치, Paralichthys olivaceus에서 분리된 VHSV(Viral Hemorrhagic Septicemia Virus)의 유전학적 검토김수미, 박수일, 박헌식, 이재일, 홍미주수산생명의학회 · 한국어병학회지2003 · 제16권 제1호남.서해안과 동중국해 자연산 어류에서 Viral hemorrhagic septicemia virus (VHSV) 검출김석렬, 오명주, 윤현미, 이월라, 정성주수산생명의학회 · 한국어병학회지2007 · 제20권 제3호우리 나라 연근해 자연산 해수 어종에서의 Viral Hemorrhagic Septicemia Virus (VHSV)의 검출김수미, 박수일수산생명의학회 · 한국어병학회지2004 · 제17권 제1호2015년 제주도 양식 넙치(Paralichthys olivaceus)를 대상으로 한 VHS 및 RSIVD 모니터링김승민, 박명애, 박신후, 박현경, 전려진, 정준범, 정현도, 조미영, 황성돈한국수산과학회 · 한국수산과학회지2016 · 제49권 제2호양식 넙치, Paralichthys olivaceus에서 분리한 viral hemorrhagic septicemia virus (VHSV)의 항원성 분석김수미, 김진우, 박수일, 원경미, 조미영, 지보영수산생명의학회 · 한국어병학회지2011 · 제24권 제2호

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