남.서해안과 동중국해 자연산 어류에서 Viral hemorrhagic septicemia virus (VHSV) 검출
Detection of Viral Hemorrhagic septicemia virus (VHSV) from marine fish in the South Western Coastal Area and East China Sea
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초록
Viral Hemorrhagic Septicemia Virus (VHSV)는 유럽과 북미지역의 담수 무지개송어에 심각한 바이러스성 질병을 야기하는 원인체였으나 담수어류 뿐만 아니라 해산어류에서도 심각한 질병을 유발하며 최근 일본과 우리나라 양식 넙치에서 VHSV에 의한 대량 폐사 발생이 보고되고 있다. 본 연구는 VHS에 대한 예방학적 접근의 일환으로서 자연산 어류로부터의 VHSV 검출 및 지리적 분포, 보균 어종에 대한 유전학적인 조사를 목적으로 2003년과, 2005년도에 동중국해역 3지점, 서해안 5지점 그리고 남해안 5지점에서 어류를 채집하였다. RT-PCR을 이용한 연구결과 자연산 해산어류 160마리 중 12종 17마리에서 VHSV가 검출되어 검출율 10.6%를 보였으며 조사된 12종의 어류 중 특히 상어목에서 가장 높은 검출율을 보였다. VHSV G gene을 이용한 계통분류학적 분석결과 염기서열 분석에서 양식산어류의 분리주 (KVHS01-1)와 98.4%～99%, 아미노산 분석에서 97～99% 유사성을 나타내어 본 연구에서 조사되어진 자연산 어류에서 분리된 VHSV는 GenogroupⅠ에 속하였다.
Abstract
Viral hemorrhagic septicemia (VHS) is one of the most serious viral disease of farmed rainbow trout and some marine fishes in Europe and North America. It has been reported in various marine fish species of Asian countries and induced cause mass mortality in Japanese flounder (Paralichthys olivaceus) culturing in Korea. The aims of this study were to monitor VHSV in wild marine fishes and to give critical information for controling the disease through prophylactic methods. Prevalence of the viral disease, geological distribution and reservoir of the virus were investigated using wild marine fishes captured in southern coast and east china sea for two years. (Reverse Transcriptase Polymerase Chain Reaction) RT-PCR results showed that VHSV were detected in 17 (10.6%) out of 160 fish. G gene sequences of viral strains isolated in this study were closely related to that of a reference strain, KVHS01-1, belonging to VHSV genotype Ⅰ. The results suggest that some of wild marine fishes are VHSV carriers and may spread the pathogen directly to fish farmed in coastal area.
참고문헌 (20)
- Benmansour, A, "Sequence variation of the glycoprotein gene identifids three distinct lineages within field isolates of viral hemorrhagic septicaemia virus, a fish rhabdovirus", 78, pp.2837~2846 (1997)
- Betts, A.M, "Nucleotide sequence analysis of the entire ding regions of virulent and avirulent strains of viral hemorrhagic septicaemia virus", 20, pp.259~262 (2000)
- Dixon. P.F, "Isolation of viral hemorrhagic septicaemia virus from Atlantic herring Clupea harengus form the English Channel", 30, pp.81~89 (1997), Dis. Apuat. Org
- Isshiki T, "An outbreak of VHSV (Viral hemorrhagic septicemia virus) infection in farmed Japanese flounder Paralichthys olivaceus in Japan", 47, pp.87~99 (2001), Dis Aquat Org
- Jensen N.J, "1979. The ulcus-syndrome in cod", pp.436~442 (1979)
- Kim, S.M, "Detection of Viral Hemorrhagic Septicemia Virus (VHSV) in wild marine fishes in the coastal region of Korea", 17, pp.1~10 (2004)
- Kim, S.M, "Genetic relationship of the VHSV (viral hemorrhagic septicemia virus) isolated from cultured olive flounder, Paralichthys olivaceus in Korea", 16, pp.1~12 (2003)
- King, J.A, "Experimental susceptibility of Atlantic salmin Salmo salar and turbot Scophthalmus maximus to European freshwater and marine isolates of viral hemorrhagic septicemia virus", 29, pp.25~31 (2001), Dis Aquat Org
- Meyers, T. R, "Isolation of the North American strain of viral hemorrhagic septicemia virus (VHSV) associated with epizootic mortality inspecies of Alaskan marine fish", 38, pp.81~86 (1999), Dis Aquat Org
- Miller, T, A, "Rapid and sensitive reverse transcriptase polymerase chain reaction based detection and differential diagnosis of fish pathogenic rhabdoviruses in organ samples and cultured cells", 34, pp.13~20 (1998), Dis. Aquat. Org
- Mortensen, H.F, "Isolation of viral hemorrhagic septicaemia virus (VHSV)from wild marine fish species in the Baltic Sea, Kattegat, Skagerrak and the North Sea", 63, pp.95~106 (1999)
- Nishizawa, T, "Genetic relatedness among Japanese, American and European isolates of viral hemorrhagic septicemia virus(VHSV) based on partial G and P genes", 48, pp.143~148 (2002), Dis. Aquat. Org
- Ross K, "1994. An outbreak of viral hemorrhagic septicaemia", pp.213~214 (1994)
- Schlotfeldt HJ, "1991. Occurrence of viral hemorrhagic septicaemia in turbot", pp.105~107 (1991)
- Stone D.M, "Nucleotide sequence of the glycoprotein gene of viral hemorrhagic septicaemia (VHS) viruses from different geographical areas: a link between VHS in farmed fish species and viruses isolated from North Sea cod(Gandus morhua)", 78, pp.1319~1326 (1997)
- Takafumi I, "Virulence of Viral Hemorrhagic Septicemia Virus (VHSV) isolates from Japanese Flounder Paralichthys olivaceus in Rainbow Trout and several species of Marine Fish", 39, pp.103~2004
- Takano R, "Isolation of viruses from wild Japanese flounder paralichthys olivaceus", 36, pp.153~160 (2001)
- Takano R, "Isolation of viral hemorrhagic septicemia virus (VHSV) from wild Japanese flounder, Paralichthys olivaceus", 20, pp.186~193 (2000)
- Watanabe L, "Isolation of aquabirnavirus and viral hemorrhagic septicemia virus (VHSV) from wild marine fishes", 37, pp.189~191 (2002)
- Watanabe L, "Isolation of aquabirnavirus and viral hemorrhagic septicemia virus (VHSV) from wild marine fishes", 37, pp.189~191 (2002)