2015년 제주도 양식 넙치(Paralichthys olivaceus)를 대상으로 한 VHS 및 RSIVD 모니터링
Monitoring of VHS and RSIVD in Cultured Paralichthys olivaceus of Jeju in 2015
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참고문헌
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초록
In this study, disease surveillance was performed to monitor the prevalence of viral haemorrhagic septicaemia virus (VHSV) and red seabream iridovirus (RSIV) in olive flounder, Paralichthys olivaceus in 2015. The fish samples were collected in March (60 farms), May (55 farms) and July (52 farms) from different farms in Jeju. Reverse transcription polymerase chain reaction (RT-PCR) (VHSV) or PCR (RSIV) results showed that VHSV detected in 2 farms, but RSIV has not been detected in any farms. The sequences of the nucleocapsid protein (N) and glycoprotein (G) gene of the 2 VHSV isolates were successfully sequenced. Phylogenetic analysis was included VHSV isolates reported here together with a representative VHSV isolates available in GenBank. Phylogenetic analysis indicated that most of Korea VHSV isolates were closely related to the Japan and China genotype Ⅳa which is clearly distinct from the North American genotype Ⅳb.
Abstract
In this study, disease surveillance was performed to monitor the prevalence of viral haemorrhagic septicaemia virus (VHSV) and red seabream iridovirus (RSIV) in olive flounder, Paralichthys olivaceus in 2015. The fish samples were collected in March (60 farms), May (55 farms) and July (52 farms) from different farms in Jeju. Reverse transcription polymerase chain reaction (RT-PCR) (VHSV) or PCR (RSIV) results showed that VHSV detected in 2 farms, but RSIV has not been detected in any farms. The sequences of the nucleocapsid protein (N) and glycoprotein (G) gene of the 2 VHSV isolates were successfully sequenced. Phylogenetic analysis was included VHSV isolates reported here together with a representative VHSV isolates available in GenBank. Phylogenetic analysis indicated that most of Korea VHSV isolates were closely related to the Japan and China genotype Ⅳa which is clearly distinct from the North American genotype Ⅳb.
참고문헌 (25)
- 조미영, "2005년부터 2006년 사이 우리나라 양식 넙치, Paralichthys olivaceus의 세균성 질병에 대한 역학 조사", 한국어병학회지, 20(1), pp.61~70 (2007), 한국어병학회
- 조미영, "2008년 우리나라 연근해산 어류에 대한 병원체 모니터링", 한국어병학회지, 22(1), pp.75~83 (2009), 한국어병학회
- 조미영, "2005년부터 2007년 사이 양식 넙치, Paralichthys olivaceus를 대상으로 한 어류바이러스 검출에 대한 통계 자료", 한국어병학회지, 23(2), pp.155~163 (2010), 한국어병학회
- OB Dale, "Outbreak of viral haemorrhagic septicaemia (VHS) in seawater-farmed rainbow trout in Norway caused by VHS virus Genotype III", Diseases of Aquatic Organisms, 85, pp.93~103 (2009), Inter-Research Science Center[DOI]
- Jeong Wan Do, "Complete genomic DNA sequence of rock bream iridovirus", Virology, 325(2), pp.351~363 (2004), Elsevier BV[DOI]
- Do JW, "Phylogenetic analysis of the major capsid protein gene of iridovirus isolates from cultured flounders Paralichthys olivaceus in Korea", Dis Aquat Org, 64, pp.193~200 (2005)
- Einer-Jensen K, "Evolution of the fish rhabdovirus viral haemorrhagic septicaemia virus", J Gen Virol, 85, pp.1167~1179 (2004)[DOI]
- Jian G. He, "Complete Genome Analysis of the Mandarin Fish Infectious Spleen and Kidney Necrosis Iridovirus", Virology, 291(1), pp.126~139 (2001), Elsevier BV[DOI]
- Inouye K, "Inidovirus infection of cultured red seabream, Pagrus major", Fish Pathol, 27, pp.19~27 (1992)
- Isshiki T, "An outbreak VHSV (viral haemorrhagic septicaemia virus) infection in farmed Japanese flounder Paralichthys olivaceus in Japan", Dis Aquat Org, 47, pp.87~99 (2001)
- Kim HJ, "Validation of the sensitivities of one-step and two-step reverse-transcription PCR methods for detection of viral haemorrhagic cepticaemia virus (VHSV) Ⅳa isolates from cultured olive flounder in Korea", Aquaculture, 448, pp.359~364 (2015)
- 김수미, "우리 나라 양식 넙치, Paralichthys olivaceus에서 분리된 VHSV(Viral Hemorrhagic Septicemia Virus)의 유전학적 검토", 한국어병학회지, 16(1), pp.1~12 (2003), 한국어병학회
- Wi-Sik Kim, "An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea", Aquaculture, 296(1-2), pp.165~168 (2009), Elsevier BV[DOI]
- 김위식, "Genetic positioning of Korean viral hemorrhagic septicemia virus (VHSV) from cultured and wild marine fishes", 한국어병학회지, 24(1), pp.1~9 (2011), 한국어병학회
- Lumsden JS, "Mortality event in freshwater drum Aplodinotus grunniens from Lake Ontario, Canada, associated with viral haemorrhagic septicaemia virus, Type IV", Dis Aquat Org, 76, pp.99~111 (2007)
- Matsukoa S, "Cultured fish species infected by red seabream iridoviral disease from 1991 to 1995", J Fish Pathol, 31, pp.233~234 (1996)
- Mortensen HF, "Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild marine fish species in the Baltic Sea, Kattegat, Skagerrak and the North Sea", Virus Res, 63, pp.95~106 (1999)
- Nishizawa T, "Genetic relatedness among Japanese, American and European isolates of viral haemorrhagic septicaemia virus (VHSV) based on partial G and P genes", Dis Aquat Org, 48, pp.143~148 (2002)
- 박현경, "2014년 제주도 양식 넙치, Paralichthys olivaceus에 대한 VHS 및 RSIVD 모니터링", 수산해양교육연구, 27(3), pp.879~889 (2015), 한국수산해양교육학회
- Schutze H, "Complete genomic sequence of viral haemorrhagic septicaemia virus, a fish rhabdovirus", Virus Genes, 19, pp.59~65
- Shi CY, "The first report of an iridovirus-like agent infection in farmed turbot Scophthalmus maximus in China", Aquaculture, 236, pp.11~25 (2004)[DOI]
- Snow M, "Analysis of the nucleoprotein gene identifies distinct lineages of viral haemorrhagic septicaemia virus within the European marine environment", Virus Res, 63, pp.35~44 (1999)
- Takano R, "Isolation of viral haemorragic septicaemia (VHSV) from wild Japanese flounder, Paralichthys olivaceus", Bull Eur Assoc Fish Pathol, 20, pp.186~192 (2007)
- Tamura K, "Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0", Mol Biol Evol, 24, pp.1596~1599 (2007)
- Thompson JD, "Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice", Nucleic Acids Res, 22, pp.4673~4680 (1994)