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우리 나라 연근해 자연산 해수 어종에서의 Viral Hemorrhagic Septicemia Virus (VHSV)의 검출

Detection of viral hemorrhagic septicemia virus (VHSV) in wild marine fishes in the coastal region of Korea

박수일|김수미
수산생명의학회 (Aquatic Life Medical Science)|2004.04|한국어병학회지 (JOURNAL OF FISH PATHOLOGY)|제17권 제1호|pp. 1-10

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초록

해수 환경 중의 VHSV 분포 조사를 위하여, 2003년 2월에서 5월까지 동해 및 남해안 인근 해역에 서식하는 자연산 해수 어류를 채집하였다. 바이러스 분리용 시료는 9종의 해수 어류로서 42개의 시료를 분석하였다. 각 시료의 조직여과액을 Epithelioma papulosum cyprini (EPC) cells에 접종한 결과, 고등어 Scomber japonicus와 숭어 Mugil cephalus에서 바이러스가 분리되었으며 이들 바이러스는 RT-PCR법을 이용하여 VHSV로 동정할 수 있었다. 고등어에서 VHSV가 검출되었다는 기보고는 있지만, 숭어에 대해서는 본 연구에서 처음으로 VHSV를 분리하였다. 자연산 어류에서 유래된 세 개의 VHSV isolates에 대한 glycoprotein gene을 분석한 결과, 이들 바이러스는 양식 넙치에서 질병을 유발하는 VHSV isolates와 유사하며 모두 genogroup I (American type)에 속하는 것으로 밝혀졌다.

Abstract

In order to analyse the detection of viral hemorrhagic septicemia virus (VHSV) in marine environment surrounding coastal region of Eastern and Southern sea of Korea, the pools of each organ sample of three fish were taken for virus assay from February to May in 2003. The sample comprised 42 samples, taken from 9 species of marine fishes. The VHSV was detected from chub mackerel Scomber japonicus and striped mullet Mugil cephalus in epithelioma papulosum cyprini (EPC) cells. The identity of the virus was confirmed a reverse transcriptase-polymerase chain reaction (RT-PCR). VHSV has previously been reported from chub mackerel, but not from striped mullet. The new isolates was classified as a member of genogroup I (American type) of VHSV and was closely related to the VHSV KVHS'01-1 based on comparisons of the partial nucleotide sequence of the glycoprotein (G) gene.

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관련 논문AI추천

남.서해안과 동중국해 자연산 어류에서 Viral hemorrhagic septicemia virus (VHSV) 검출김석렬, 오명주, 윤현미, 이월라, 정성주수산생명의학회 · 한국어병학회지2007 · 제20권 제3호Genetic positioning of Korean viral hemorrhagic septicemia virus (VHSV) from cultured and wild marine fishes김두운, 김위식, 김정호, 김종오, 오명주, 정성주수산생명의학회 · 한국어병학회지2011 · 제24권 제1호우리 나라 양식 넙치, Paralichthys olivaceus에서 분리된 VHSV(Viral Hemorrhagic Septicemia Virus)의 유전학적 검토김수미, 박수일, 박헌식, 이재일, 홍미주수산생명의학회 · 한국어병학회지2003 · 제16권 제1호해수 사육 무지개송어(Oncorhynchus mykiss)의 Viral Hemorrhagic Septicemia Virus (VHSV) 모니터링공경희, 김위식, 오명주, 전영호한국수산과학회 · 한국수산과학회지2017 · 제50권 제5호아시아에서 분리된 viral haemorrhagic septicaemia virus (VHSV) isolates의 계통분석학적 비교박명애, 안상중, 조미영, 지보영수산생명의학회 · 한국어병학회지2013 · 제26권 제3호2015년 제주도 양식 넙치(Paralichthys olivaceus)를 대상으로 한 VHS 및 RSIVD 모니터링김승민, 박명애, 박신후, 박현경, 전려진, 정준범, 정현도, 조미영, 황성돈한국수산과학회 · 한국수산과학회지2016 · 제49권 제2호

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