참비름(Amaranthus mangostanus ) 추출물의 미백 및 주름 개선 효과
Skin Whitening and Anti-wrinkle Effects of Chambirum (Amaranthus mangostanus)
0
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
목적: 나물로만 사용되는 참비름(Amaranthus mangostanus) 추출물을 대상으로 화장품 소재적 특성을 조사하기 위하여 항산화 효과, 미백 활성 및 주름개선 효능을 진행하였다. 방법: 항산화 활성으로는 DPPH와 SOD-like activity, 미백활성으로는 tyrosinase 저해 활성과 melanin 함량을 조사하였고, 주름개선 효능으로는 elastase 저해 활성과 type 1 pro-collagen을 측정하였다. B16F10 mouse melanoma cells과 CCD986sk human dermal fibroblast cells을 대상으로 세포 생존력을 측정하였다. 결과: DPPH 방법으로조사한 항산화 활성은 시료의 농도 500 μg/mL의 농도에서 AME (49%), AMW (56%)의 radical 소거 활성을 나타내었고, SOD-like activity를 측정한 결과, 시료의 농도는 동일하게 500 μg/mL에서 AME (67%), AMW (57%)의 활성을 나타내었다. 미백활성으로는L-tyrosine을 기질로 사용한 tyrosinase 저해 활성은 AME (25.9%), AMW (23.4%)로 나타내었고 L-DOPA의 경우 AME (17.8%), AMW (18.5%)의 높은 미백효과를 확인 할 수 있었다. B16F10 mouse melanoma cell line을 대상으로 melanin양을 측정한 결과, AME (25.5%), AMW (27.8%)의 melanin 감소율을 나타내었다. 주름개선 효능으로는, 시료의 농도, 500 μg/mL에서 elastase 저해활성은 AME (19.7%), AMW (17.2%)로 조사되었고, type I procollagen의 collagen 양은 미미한 것으로 나타내었다. B16F10 mouse melanoma cells에 대한 세포 독성은 시료의 농도 100 μg/mL에서 AME (76%), AMW (86%)의 세포 생존율을 나타내었고, CCD- 986sk fibroblast cells에 대한 세포독성 결과는 시료의 농도 100 μg/mL에서 AME (94.5%), AMW (83.6%)의 생존율을 확인할 수 있었다. 결론: 참비름 추출물은 항산화 및 미백 활성에 탁월한 효능을 보여주었고, 천연물 유래의 화장품 소재로서의 가능성과 항노화산업의 잠재적인 소재로서의 가치를 확인할 수 있었다.
Abstract
To investigate the cosmetic effects of a chambirum (Amaranthus mangostamus ) extract on the skincare by measuring the extract’s antioxidant, antiwrinkle, and whitening. Methods: To confirm the antioxidant effect of chambirum, DPPH radical scavenging activity and SOD-like activity was determined. To confirm whitening effect of chambirum, tyrosinase inhibitory activity and melanin content were investigated. To confirm anti-wrinkle effect of chambirum, the elastase inhibition effect and type I procollagen production was assayed. B16F10 mouse melanoma cells and CCD986sk human dermal fibroblast cells were used to assay cell viability. Results: The DPPH radical scavenging activity of AME and AMW was 49% and 56%, respectively, at 500 μg/mL. The SOD-like activity of AME and AMW was 67% and 57%, respectively, compared with that of the standard sample L-ascorbic acid at 38% at 1 mM. AME showed 25.9% (L-tyrosine) and 17.8% (L-DOPA), and AMW showed 23.4% (L-tyrosine) and 18.5% (L-DOPA) of tyrosinase inhibition assay. In addition, the intracellular melanin production in B16F10 mouse melanoma cells was decreased by AME and AMW by 25.5% and 27.8%, respectively. Additionally, the elastase inhibition assay results demonstrated inhibition by AME (19.7%) and AMW (17.2%) at 500 μg/mL and the amount of type I procollagen was shown to be insignificant. Furthermore, B16F10 mouse melanoma cells were 76% and 86% showed a viability 76% and 86% when treated with 100 μg/mL AME and AMW, respectively. CCD-986sk fibroblast cells showed a viability 94.5% and 83.6% when we treated with 100 μg/mL AME and AMW, respectively. Conclusion: These results suggest that the chambirum (Amaranthus mangostamus) extract has excellent antioxidants, anti-wrinkle, and whitening effects. Therefore, it may have a significant industrial value as a functional material for cosmetic or anti-aging related bioproducts.
참고문헌 (35)
- Ashok Kumar BS, "In vitro alphaamylase inhibition and in vivo antioxidant potential of Amaranthus spinosus in alloxan-induced oxidative stress in diabetic rats", Saudi Journal of Biological Sciences, 8, pp.1~5 (2011)
- Bieth J, "The synthesis and analytical use of a high sensitive and convenient substrate of elastase", Biochemical Medicine, 11, pp.350~357 (1974)
- Cho KJ, "Purification and characterization of antiviral protein(AAP29)from the leaves of Amaranthu mangostanus", Applied Biological Chemistry, 38, pp.528~533 (1995)
- Choi SY, "Emerging technology : development of skin depigmenting materials using food", Bulletin of Food Technology, 25, pp.150~153 (2012)
- Fan Y, "Hyaluronic acidcross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β", Smad signaling pathway in a nude mouse model, 2019, pp.72, 1355~1362
- Fasuyi AO, "Bio-nutritional evaluations of three tropical leaf vegetables(Telfairia occidentalis, Amaranthus cruentus and Talinum triangulare)as sole dietary protein sources in rat assay", Food Chemistry, 103, pp.757~765 (2007)
- Grajeta H, "Effect of amaranth and oat bran on blood serum and liver lipids in rats depending on the kind of dietary fats", Nahrung, 43, pp.114~117 (1999)
- 한지영, "포도씨 추출물과 분획물의 Tyrosinase 저해활성", 한국식품영양과학회지, 37(12), pp.1679~1683 (2008), 한국식품영양과학회
- Hearing VJ, "Mammalian tyrosinase. A comparison of tyrosine hydroxylation and melanin formation", The Biochemical Journal, 157, pp.549~557 (1976)
- Hearing VJ, "Mammalian tyrosinase : the critical regulatory control point in melanocyte pigmentation", International Journal of Biochemistry, 19, pp.1141~1147 (1987)
- Holmberg D, "The effect of amaranth treatment on some kidney and liver enzymes in the rat", Toxicology and Applied Pharmacology, 46, pp.257~260 (1978)
- Hosoi J, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid", Cancer Research, 45, pp.1474~1478 (1985)
- 찌아위엔, "Allium속 식물 물추출물의 항산화 활성과 Tyrosinase 및 Elastase 저해 효과", 한국식품영양학회지, 28(2), pp.247~252 (2015), 한국식품영양학회
- 곽정훈, "치자 열매 추출물의 Tyrosinase 효소활성 저해 및 Melanogenesis 억제 효과", KSBB Journal, 19(6), pp.437~440 (2004), 한국생물공학회
- Kim HK, "Antioxidative antidiabetic effects of amaranth(Amarnthus esculantus)in streptozotocin-induced diabetic rats", Cell Biochemistry and Function, 24, pp.195~199 (2006)
- 김재원, "유산균 발효한 현미상황버섯균사체 추출물의 β-Glucan 함량 및 주름개선 효과", 아시안뷰티화장품학술지, 14(2), pp.127~137 (2016), 한국피부과학연구원[DOI]
- 김영주, "인간진피섬유아세포에서 천궁추출물의 MMP1 발현 조절 기전", 아시안뷰티화장품학술지, 16(1), pp.131~138 (2018), 한국피부과학연구원[DOI]
- 김윤정, "Whitening and Moisturizing Effects of Hydrolyzed Swiftlet Nest Extracts", 아시안뷰티화장품학술지, 19(4), pp.639~649 (2021), 한국피부과학연구원[DOI]
- Lam LK, "Citrus limonoid reduction of chemically induced tumorigenesis", Food Technology, 48, pp.104~108 (1994)
- Lee BJ, "Functional cosmetics market trend", Korean Industrial Chemistry News, 20, pp.46~47 (2017)
- 이진영, "Comparison of Biological Activity between Nelumbo nucifera G. Extracts and Cosmetics Adding Nelumbo nucifera G.", 생명과학회지, 20(8), pp.1241~1248 (2010), 한국생명과학회
- Lee KS, "nc886, a non-coding RNA, inhibits UVB-induced MMP-9 and COX-2 expression via the PKR pathway in human keratinocytes", Biochemical and Biophysical Research Communications, 512, pp.647~652 (2019)
- Malherbe C J, "Iriflophenone-3-C-glucoside from Cyclopia genistoides : isolation and quantitative comparison of antioxidant capacity with mangiferin and isomangiferin using on-line HPLC antioxidant assays", Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences, 951, pp.164~171 (2014)
- Marklund S, "Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", European Journal of Biochemistry, 47, pp.469~474 (1974)
- 오영숙, "참비름 추출물에서 항균성 물질의 분리 및 동정", 한국미생물·생명공학회지, 33(2), pp.123~129 (2005), 한국미생물·생명공학회
- Pacifico S, "Antioxidant evaluation of polyhydroxylated nerolidols from redroot pigweed(Amaranthus retroflexus)leaves", Food Science and Technology Letters, 41, pp.1665~1671 (2008)
- 박의균, "Effects of Unripe Apple Polyphenols on the Expression of Matrix Metalloproteinase-1 and Type-1 Procollagen in Ultraviolet Irradiated Human Skin Fibroblasts", Applied Biological Chemistry, 57(4), pp.449~455 (2014), 한국응용생명화학회
- Plate AYA, "Cholesterol-lowering effect of extruded amaranth (Amaranthus caudatus L.) in hypercholesterolemic rabbits", Food Chemistry, 76, pp.1~6 (2002)
- Sangameswaran B, "Anti-diabetic, antihyperlipidemic and spermatogenic effects of Amaranthus spinosus Linn on streptozotocin-induced diabetic rats", Journal of Natural Medicines, 62, pp.79~82 (2008)
- Sephel GC, "Elastin production in human skin fibroblast cultures and its decline with age", Journal of Investigative Dermatology, 86, pp.279~285 (1986)
- Sharma OP, "DPPH antioxidant assay revisited", Food Chemistry, 113, pp.1202~1205
- Sugumaran M., "Comparative biochemistry of eumelanogenesis and the protective roles of phenoloxidase and melanin in insects", Pigment Cell Research, 15, pp.2~9 (2002)
- Tsuji N, "The role of elastases secreted by fibroblasts in wrinkle formation : implication through selective inhibition of elastase activity", Photochemistry Photobiology, 74, pp.283~290 (2001)
- Videira IFS, "Mechanisms regulating melanogenesis", Anais Brassileiros de Dermatologia, 88, pp.76~83 (2013)
- Yang J, "Growth of cultured cells using collagen as substrate", International Review of Cytology, 81, pp.249~286 (1983)