고등학교 생명과학 실험 교과 운영을 위한 유전자 재조합 실험 프로그램 개발

Abstract

This study presents a DNA recombination experiment program developed to efficiently implement the Life Science Experiment course in line with the 2022 Revised Curriculum and the High School Credit System, both of which will be introduced in 2025. The program involves cutting a gene of interest and a plasmid DNA with the same restriction enzyme to create complementary sticky ends. Subsequently, DNA electrophoresis is conducted, and the gene of interest and the plasmid DNA are extracted from an agarose gel and ligated. The ligated plasmid is then transformed into competent E. coli, and activities are performed to screen the transformed E. coli with the recombinant plasmid. The plasmid DNA contains an ampicillin resistance gene and the arabinose operon. Since the gene of interest synthesizes the green fluorescent protein, the transformed E. coli with the recombinant DNA can be easily identified by culturing it on LB agar plates containing ampicillin and arabinose and observing it under UV light. To analyze the learning effectiveness of the developed DNA recombination experiment program, evaluation items were created to assess whether students met the achievement standards related to DNA recombination in the 2022 Revised Curriculum Life Science Experiment course. The analysis showed a significant improvement in achievement levels related to these concepts after the class compared to before. Additionally, using the TOSRA questionnaire, a scientific attitude test was conducted, revealing positive impacts across all seven areas. Therefore, the DNA recombination experiment program developed in this study was found to be appropriate for the Life Science Experiment course, aiding in achieving related achievement standards and fostering positive attitudes toward life sciences.