고등학생 교육용 대장균 형질 전환 실험 교재 개발 및 적용
Development of the Escherichia coli Transformation and Its Application for a High School Classroom Exercise
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The first challenge in designing and teaching science is to decide what to teach. The goals for what students should learn are not only what is taught, but also how it is taught. Although many experiments are included in the current curriculum, it is difficult for students to perform them because lab in general high school is not equipped with adequate experimental apparatus. However, exposing students to scientific process is important for students to connect between science concepts and applications. The rapidly expanding biotechnology rests upon recombinant DNA technology. To introduce the development of biotechnology in science field, the recombinant DNA technology has been included in high school science textbook. Recombinant DNA technology can be challenging to implement within the limitations of an educational lab, but we have optimized this experimental method to work in a teaching lab environment and to maximize the “hands-on" experience for the high school students. In this study, we have developed an E. coli transformation experiment that can easily be performed in high school classroom. This experiment give a chance to students to explore the scientific method and to understand genetic principles that are derived from the method.To evaluate educational effect of this simplified E. coli transformation experiment, we examined student outcomes in three distinct areas-achievement, understanding of practical performance, and attitude for biotechnology. The experiment in this study was helpful for students to improve scores in the written test and have positive view to biotechnology, suggesting that it can be useful in classroom experiment.
Abstract
The first challenge in designing and teaching science is to decide what to teach. The goals for what students should learn are not only what is taught, but also how it is taught. Although many experiments are included in the current curriculum, it is difficult for students to perform them because lab in general high school is not equipped with adequate experimental apparatus. However, exposing students to scientific process is important for students to connect between science concepts and applications. The rapidly expanding biotechnology rests upon recombinant DNA technology. To introduce the development of biotechnology in science field, the recombinant DNA technology has been included in high school science textbook. Recombinant DNA technology can be challenging to implement within the limitations of an educational lab, but we have optimized this experimental method to work in a teaching lab environment and to maximize the “hands-on" experience for the high school students. In this study, we have developed an E. coli transformation experiment that can easily be performed in high school classroom. This experiment give a chance to students to explore the scientific method and to understand genetic principles that are derived from the method.To evaluate educational effect of this simplified E. coli transformation experiment, we examined student outcomes in three distinct areas-achievement, understanding of practical performance, and attitude for biotechnology. The experiment in this study was helpful for students to improve scores in the written test and have positive view to biotechnology, suggesting that it can be useful in classroom experiment.
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