사염화탄소로 유도된 간 손상 동물모델에서 차가버섯 열수 추출물이 면역 조절 기능에 미치는 영향
Immunoregulatory Effects of Water Extracts of Inonotus obliquus in Carbon Tetrachloride-Induced Liver Damage Animal Model
2
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
Inonotus obliquus is one of the immune-regulatory substances and is recognized to play the role in the metabolic process of inflammation, allergy and immuntiy. The purpose of this study was to evaluate the effects of water extracts of Inonotus obliquus (IOW) on the liver lymphocyte immune function in the Sprague-Dawley male rats treated with carbon tetrachloride (CCl4) to induce liver damage. Rats were fed with each experimental diet and water for 4 weeks. We found that effects of IOW on interferon-gamma (IFN-γ), signal transducer and activator of transcription 1 (STAT1), phospho-signal transducer and activator of transcription 1 (pSTAT1) and GATA-binding protein 3 (GATA-3) were decrease in vivo. Interleukin-4 (IL-4), STAT6, pSTAT6 and T-box expressed in T-cells (T-bet) decreased significantly lower in CCl4 + IOW group than the CCl4 group. Our data indicated that cytokine protein production were increased in CCl4 group and CCl4 + IOW group. As a result of this study, we assume that IOW fed could regulate the immuno-modulating functions through regulate the cytokine production capacity activated by liver damage.
Abstract
Inonotus obliquus is one of the immune-regulatory substances and is recognized to play the role in the metabolic process of inflammation, allergy and immuntiy. The purpose of this study was to evaluate the effects of water extracts of Inonotus obliquus (IOW) on the liver lymphocyte immune function in the Sprague-Dawley male rats treated with carbon tetrachloride (CCl4) to induce liver damage. Rats were fed with each experimental diet and water for 4 weeks. We found that effects of IOW on interferon-gamma (IFN-γ), signal transducer and activator of transcription 1 (STAT1), phospho-signal transducer and activator of transcription 1 (pSTAT1) and GATA-binding protein 3 (GATA-3) were decrease in vivo. Interleukin-4 (IL-4), STAT6, pSTAT6 and T-box expressed in T-cells (T-bet) decreased significantly lower in CCl4 + IOW group than the CCl4 group. Our data indicated that cytokine protein production were increased in CCl4 group and CCl4 + IOW group. As a result of this study, we assume that IOW fed could regulate the immuno-modulating functions through regulate the cytokine production capacity activated by liver damage.
참고문헌 (34)
- Abbas AK, "Functional diversity of helper T lymphocytes", Nature, 383, pp.787~793 (1996)
- Agnello D, "Cytokines and transcription factors that regulate T helper cell differentiation: new players and new insights", Journal of Clinical Immunology, 23, pp.147~161 (2003)
- Ashburm LL, "The nonportal distribution of trabecule in dietary cirvhosis of mouse and huinea pigs", American Journal of Pathology, 23, pp.159~ (1947)
- Burnette WN, ""Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A", Analytical Biochemistry, 112, pp.195~203 (1981)
- Butler TC, "Reduction of carbon tetrachloride in vivo and reduction of carbon tetrachloride and chloroform in vitro by tissue and tissues constituents", The Journal of Pharmacology and Expermental Therapeutics, 134, pp.311~319 (1990)
- Cha JY, "Effect of fermented compositions containing Inonotus obliquus with Houttuynia cordata on growth of human AGS gastric and HCT-15 colon cancer cells", Journal of the Korean Society for Applied Biological Chemistry, 47, pp.202~207 (2004)
- Jae-Young Cha, "Fermented Chaga Mushroom (Inonotus obliquus) Effects onHypolipidemia and Hepatoprotection in Otsuka Long-Evans TokushimaFatty (OLETF) Rats", Food Science and Biotechnology, 15(1), pp.122~127 (2006), 한국식품과학회
- Cho MH, "The base of toxicology", pp.116~120 (2004), Young Chi
- Escoubet-Lozach L, "The role of transcription factors in allergic inflammation", Journal of Allergy and Clinical Immunology, 110, pp.553~564 (2002)
- Falcone M, "Cytokines that regulate auto-immune responses", Current Opinion in Immunology, 11, pp.670~676 (1999)
- Finotto S, "Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet", Science, 295, pp.336~338 (2002), 336-338.
- 함승시, "차가버섯 분획물의 항돌연변이 활성 및 암세포 성장억제효과", 한국식품영양과학회지, 32(7), pp.1088~1094 (2003), 한국식품영양과학회
- Kay AB, "Leukocytes in asthma", Immunol Invest, 17, pp.679~705 (1988)
- Kim HA, "Immunotoxicological effects of Agent Orenge exposure to the Vietnam War Korean veterans", Industrial Health, 41, pp.158~166 (2003)
- Kim SW, "Studies on anti-microbial and anti-cancer function of polysaccharide extracted Ganoderma lucidum", Journal of the Korean Society of Food Science and Nutrition, 27, pp.1183~1188 (1998)
- Larche M, "The role of T lympho-cytes in the pathogenesis of asthma", Journal of Allergy and Clinical Immunology, 111, pp.450~463 (2003)
- 정민호, "새로운 번데기 동충하초의 수집, 동정 및 간기능에 미치는 효과", 한국균학회지, 34(1), pp.7~14 (2006), 한국균학회
- Lee IS, "Polyzellus multiplex, a Korean wild mushroom, as a potent chemopreventive agent against stomach cancer", Life Sciences, 73, pp.3225~3234 (2003)
- 이승은, "Antioxidative and Hepatoprotective Effects of Lythrum salicaria", 한국약용작물학회지, 17(1), pp.1~7 (2009), 한국약용작물학회
- Lieber CS, "Alcohol and the liver", Gastroenterology, 106, pp.1085~1105 (1994)
- Lim BO, "Effect of the flavonoid components obtained from Scutellaria Radix on the histamine, immunoglobulin E and lipid peroxidation of spleen lymphocytes of Sprague-dawley rats", Bioscience Biotechnology and Biochemistry, 67, pp.1126~1129 (2003)
- 임병우, "한약재인 황금의 궤양성 실험동물에 대한 장간막 임파절 임파구의 면역글로블린 수준, T세포 집단, 사이토카인 생성의 조절작용", 한국약용작물학회지, 16(2), pp.100~105 (2008), 한국약용작물학회
- McCartney-Francis N, "Suppression of arthritis by an inhibitor of nitric oxide synthase", Journal of Experimental Medicine, 178, pp.749~754 (1993)
- McCay PB, "Oxygen and carbon-centered free radical formation during carbon tetrachloride metabolism", Journal of Biological Chemistry, 259, pp.2135~2143 (1984)
- Mizuno T, "Antitumor and hypohlycemic activities of polysaccharides from the Sclerotia and Mycelia of Inonotus obliquus (Pers.: Fe.) Pll. (Aphyllophoromycetideae)", International Journal of Medicinal Mushrooms, 1, pp.301~316 (1999)
- Moncada S, "Nitric oxide: physiology, pathophysiology, and pharmacology", Pharmacological Reviews, 43, pp.109~142 (1991)
- Na JC, "Effect of 12 weeks combined running and muscular resistance eExercise on physical fitness in obese female", Korean Alliance for Health, Physical Education, Recreation, and Dance., 40, pp.440~447 (1992)
- Nathan C, "Nitric oxide as a secretory product of mammalian cells", The Federation of American Societies for Experimental Biology, 6, pp.3051~3064 (1992)
- Ng ML, "Inhibition of human colon carcinoma development by lentinan from shiitake mushroom (Lentinus edodes)", Journal of Alternative and Complementary Medicine, 8, pp.581~589 (2002)
- Stuehr DJ, "Purification and characterization of the cytokine-induced macrophage nitric oxide synthase: an FAD- and FMNcontaining flavoprotein", Proceedings of the National Academy of Sciences, 88, pp.7773~7777 (1991)
- Tharp GD, "Reduction of saliva immunoglobulin levels by swim training", European Journal of Applied Physiology and Occupational Physiology, 60, pp.61~64 (1990)
- Weisz A, "Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-γ, bacterial lipopolysaccharide and NG-monomethyl- L-arginine", Biochemical Journal, 316, pp.209~215 (1996)
- Wood P, "Understanding immunology", pp.22~24 (2001), Prentice Hall
- Zhang DH, "Inhibition of allergic inflammation in a murine model of asthma by expression of a dominant-negative mutant of GATA-3", Immunity, 11, pp.473~482 (1999)