방사선 조사된 차가버섯 열수 추출물의 비장세포 증식능 및사이토카인 발현에 미치는 영향
Effect of Gamma Irradiation on the Splenocyte Proliferation and Cytokine Production of Chaga Mushroom Hot Water Extract
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초록
This study investigated the effect of gamma irradiation on immune enhancing activityof Chaga mushroom extract (CME). CME was prepared by hot water extraction at 70C for 4 hoursand lyophilized. Lyophilized CME powder was dissolved with deionized water at 10 mg ml-1 andthen irradiated at the doses of 10, 30 and 50 kGy by cobalt 60 gamma irradiator. The gammairradiatedand non-irradiated CME were treated into the splenocyte separated from mouse. Cellproliferation and cytokine production of the immune cells were increased by gamma-irradiatedCME and these increases were more prominent when CME was irradiated at higher doses. Therefore,it is considered that gamma irradiation can be an effective method for improvement of theimmunomodulating activity Chaga mushroom extract.
Abstract
This study investigated the effect of gamma irradiation on immune enhancing activityof Chaga mushroom extract (CME). CME was prepared by hot water extraction at 70C for 4 hoursand lyophilized. Lyophilized CME powder was dissolved with deionized water at 10 mg ml-1 andthen irradiated at the doses of 10, 30 and 50 kGy by cobalt 60 gamma irradiator. The gammairradiatedand non-irradiated CME were treated into the splenocyte separated from mouse. Cellproliferation and cytokine production of the immune cells were increased by gamma-irradiatedCME and these increases were more prominent when CME was irradiated at higher doses. Therefore,it is considered that gamma irradiation can be an effective method for improvement of theimmunomodulating activity Chaga mushroom extract.
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