사염화탄소로 유도된 흰쥐의 간손상으로부터 다시마(Saccharina japonica) 추출물의 보호효과
Saccharina japonica Extract Protects against Carbon Tetrachloride-in- duced Hepatotoxicity in Rats
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초록
The brown alga Saccharina japonica is consumed as a foodstuff in many countries. Carbon tetrachloride (CCl4) is a potent hepatotoxin that is used to assess hepatotoxicity in animal models. This study assessed the protective effect of S . japonica extract (SJE) on CCl4-induced acute liver injury in rats. Experimental rats were divided into the following three groups: control, CCl4, and CCl4+SJE; the latter two groups were given 150 or 300 mg SJE/kg orally for 10 days. Three hours after the final treatment, all rats-except for those in the control group-were administered intraperitoneal injections of CCl4. One day later, blood and liver samples were collected for evaluation biomarker of levels. Aspartate transaminase (APT; GPT) and alanine aminotransferase (ALT; GOT) levels were markedly lower in the CCl4+SJE group than the CCl4 group. The hepatic superoxide dismutase (SOD) activity of the CCl4+SJE group was significantly lower than that of the CCl4 group. In comparison, glutathione S-transferase (GSH) and catalase (CAT) levels were significantly higher in the CCl4+SJE group. Western blotting revealed that SJE attenuated the CCl4-induced EGFR and MAPK activity in the liver. Thus, we conclude that SJE protects against CCl4-induced hepatotoxicity.
Abstract
The brown alga Saccharina japonica is consumed as a foodstuff in many countries. Carbon tetrachloride (CCl4) is a potent hepatotoxin that is used to assess hepatotoxicity in animal models. This study assessed the protective effect of S . japonica extract (SJE) on CCl4-induced acute liver injury in rats. Experimental rats were divided into the following three groups: control, CCl4, and CCl4+SJE; the latter two groups were given 150 or 300 mg SJE/kg orally for 10 days. Three hours after the final treatment, all rats-except for those in the control group-were administered intraperitoneal injections of CCl4. One day later, blood and liver samples were collected for evaluation biomarker of levels. Aspartate transaminase (APT; GPT) and alanine aminotransferase (ALT; GOT) levels were markedly lower in the CCl4+SJE group than the CCl4 group. The hepatic superoxide dismutase (SOD) activity of the CCl4+SJE group was significantly lower than that of the CCl4 group. In comparison, glutathione S-transferase (GSH) and catalase (CAT) levels were significantly higher in the CCl4+SJE group. Western blotting revealed that SJE attenuated the CCl4-induced EGFR and MAPK activity in the liver. Thus, we conclude that SJE protects against CCl4-induced hepatotoxicity.
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