꾸지뽕나무 배양 상황버섯 자실체 및 균사체의 면역증진 효과
Immune-Enhancing Effects of Phellinus linteus Fruit Body and Mycelium Cultured in Cudrania tricuspidata
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Polysaccharides produced in microorganisms and plants are known to increase the immune response in the body. We proposed analysis of beta-glucan contents of phellinus linteus fruit body (FB) and mycelium (MC) cultured in cudrania tricuspidata. Also, we examined whether fruit body and mycelium can increase the immune response in cyclophosphamide-induced immunosuppression animal models. We injected cyclophosphamide (50 mg/kg) twice to produce immunosuppression mice. Then, FB (200 mg/kg) and MC (200 mg/kg) were oral administered for 14 days. In order to confirm the immune-enhancing effect of FB and MC, we analyzed spleen weight, the number of immune cells, cytokines, and immunoglobulins levels. Cyclophosphamide decreased the weight of spleen, the number of immune cells. However, FB and MC have significantly increased the weight of spleen, the number of white blood cell, lymphocyte and monocyte. In addition, they have significantly increased immune-related cytokines (IL-2 and IFN-γ) and immunoglobulins (IgA, IgG, IgM) levels. As a results, phellinus linteus fruit body (FB) and mycelium (MC) cultured in cudrania tricuspidata can be used as effective natural materials for immune-enhancing.
Abstract
Polysaccharides produced in microorganisms and plants are known to increase the immune response in the body. We proposed analysis of beta-glucan contents of phellinus linteus fruit body (FB) and mycelium (MC) cultured in cudrania tricuspidata. Also, we examined whether fruit body and mycelium can increase the immune response in cyclophosphamide-induced immunosuppression animal models. We injected cyclophosphamide (50 mg/kg) twice to produce immunosuppression mice. Then, FB (200 mg/kg) and MC (200 mg/kg) were oral administered for 14 days. In order to confirm the immune-enhancing effect of FB and MC, we analyzed spleen weight, the number of immune cells, cytokines, and immunoglobulins levels. Cyclophosphamide decreased the weight of spleen, the number of immune cells. However, FB and MC have significantly increased the weight of spleen, the number of white blood cell, lymphocyte and monocyte. In addition, they have significantly increased immune-related cytokines (IL-2 and IFN-γ) and immunoglobulins (IgA, IgG, IgM) levels. As a results, phellinus linteus fruit body (FB) and mycelium (MC) cultured in cudrania tricuspidata can be used as effective natural materials for immune-enhancing.
참고문헌 (32)
- Geha RS, "Primary immunodeficiency diseases : an update from the international union of immunological societies primary immunodeficiency diseases classification committee", J Allergy Clin Immunol, 120, pp.776~794 (2007)
- Sierra S, "Increased immune response in mice consuming rice bran oil", European journal of nutrition, 44(8), pp.509~516 (2005)
- Ghatak SB, "Investigation of the immunomodulatory potential of oryzanol isolated from crude rice bran oil in experimental animal models", Phytotherapy research, 26(11), pp.1701~1708 (2012)
- Kim JW, "Immunomodulatory effects of kuseonwangdogo-based mixed herbal formula extracts on a cyclophosphamide-induced immunosuppression mouse model", Evidence-Based Complementary and Alternative Medicine (2018)
- Jiang MH, "Immunoregulatory actions of polysaccharides from Chinese herbal medicine", Expert Opin Ther Tar, 14, pp.1367~1402 (2010)
- 김형숙, "Stimulatory Effect of β-glucans on Immune Cells", Immune Network, 11(4), pp.191~195 (2011), 대한면역학회
- Ikekawa T, "Antitumor action of some Basidomycetes, especially cultured Phellinus linteus", Gann, 59, pp.155~157 (1968)
- Lu TL, "Hispolon from Phellinus linteus has antiproliferative effects via MDM2-recruited ERK1", 2 activity in breast and bladder cancer cells, 2009, pp.47(8), 2013~2021
- Sliva D, "Phellinus linteus suppresses growth, angiogenesis and invasive behaviour of breast cancer cells through the inhibition of AKT signalling", British journal of cancer, 98(8), pp.1348~ (2008)
- Zhu T, "A medicinal mushroom : Phellinus linteus", Current medicinal chemistry, 15(13), pp.1330~1335 (2008)
- 이병의, "상황버섯 균사체 추출물의 면역증진 효능", 생약학회지, 43(2), pp.157~162 (2012), 한국생약학회[DOI]
- Lim ES, "A Study on the Immune activaty of Phellinus Linteus Extracts", The Journal of Korean Oriental Oncology, 8(1), pp.93~101 (2002)
- 김헌, "금사상황버섯 추출물이 혈중 IL-12, IFN-γ, TNF-α 발현에 미치는 영향", Korean Journal of Health Promotion, 6(4), pp.245~249 (2006), 대한임상건강증진학회
- Pyo MY, "Effects of Phellinus linteus extracts on the humoral immune response in normal and cyclophosphamide-treated mice", J Appl Pharmacol, 9, pp.194~200 (2001)
- 김건희, "뽕나무(Morus alba)와 꾸지뽕나무(Cudrania tricuspidata)의 부위에 따른 항산화 활성 및 3T3-L1세포 지방축적 억제 효과", 한국식품영양학회지, 32(2), pp.138~147 (2019), 한국식품영양학회[DOI]
- Heroor S, "Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils", Indian journal of pharmacology, 45(2), pp.130~ (2013)
- 정도연, "Cyclophosphamide에 의한 면역저하 동물모델에서 블루베리 효모 발효 분말의 면역증강 효과", 동의생리병리학회지, 33(1), pp.48~55 (2019), 한의병리학회[DOI]
- Promphet P, "Immune enhancement activities of silk lutein extract from Bombyx mori cocoons", Biological research, 47(1), pp.15~ (2014)
- Brown GD, "Fungal β-glucans and mammalian immunity", Immunity, 19(3), pp.311~315 (2003)
- Nitschke J, "A new colorimetric method to quantify β-1, 3-1, 6-glucans in comparison with total β-1, 3-glucans in edible mushrooms", Food chemistry, 127(2), pp.791~796 (2011)
- 조재한, "영지버섯 균주별 자실체의 베타글루칸과 폴리페놀 함량 비교 분석", 한국버섯학회지, 11(3), pp.164~170 (2013), 한국버섯학회
- Huitema AD, "The clinical pharmacology of alkylating agents in high-dose chemotherapy", Anti-Cancer Drugs, 11(7), pp.515~533 (2000)
- Cui H, "Dioscorea bulbifera polysaccharide and cyclophosphamide combination enhances anti-cervical cancer effect and attenuates immunosuppression and oxidative stress in mice", Scientific reports, 6, pp.19185~ (2016)
- Gladstone DE, "High-dose cyclophosphamide for severe systemic lupus erythematosus", Lupus, 11(7), pp.405~410 (2002)
- Cesta MF, "Normal structure, function, and histology of the spleen", Toxicologic pathology, 34(5), pp.455~465 (2006)
- Murphy K, "Janeway's immunobiology" (2016), Garland Science
- Huang JQ, "Alleviation of cyclophosphamide-induced immunosuppression in mice by naturally acetylated hemicellulose from bamboo shavings", Food and Agricultural Immunology, 28(2), pp.328~342 (2017)
- Vignali DA, "IL-12 family cytokines : immunological playmakers", Nature immunology, 13(8), pp.722~ (2012)
- Decker T, "The yin and yang of type I interferon activity in bacterial infection", Nature Reviews Immunology, 5(9), pp.675~ (2005)
- Yu Q, "Chemoprotective effects of Ganoderma atrum polysaccharide in cyclophosphamide-induced mice", International journal of biological macromolecules, 64, pp.395~401 (2014)
- Kwon HG, "Immunological homeostasis and inflammatory immune disorders", Korean Soc Mol Cell Biol, 20(1), pp.48~69 (2008)
- Wang H, "A polysaccharide from Strongylocentrotus nudus eggs protects against myelosuppression and immunosuppression in cyclophosphamide-treated mice", International immunopharmacology, 11(11), pp.1946~1953 (2011)