Protection Against Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Enteritidis Infection in Layer Chickens Conferred by a Live Attenuated Salmonella Typhimurium Strain
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In the present study, we investigated the protection conferredby a live attenuated Salmonella enterica serovarTyphimurium (ST) strain against Salmonella Typhimurium,Salmonella Gallinarum (SG), and Salmonella Enteritidis(SE) infection in layer chickens. Birds were orally primed withthe attenuated ST strain at 7 days of age and then boostedat 4 weeks post prime immunization (PPI). Sequential monitoringof plasma IgG and mucosal secretory IgA (sIgA) levelsrevealed that inoculation with ST induced a significant antibodyresponse to antigens against ST, SE, and SG. Moreover, significant lymphoproliferative responses to the 3Salmonella serovars were observed in the immunized group. We also investigated protection against virulent ST, SE, andSG strain challenge. Upon virulent SG challenge, the immunizedgroup showed significantly reduced mortality comparedto the non-immunized group. The reduced persistenceof the virulent ST and SE challenge strains in the liver,spleen, and cecal tissues of the immunized group suggeststhat immunization with the attenuated ST strain may not onlyprotect against ST infection but can also confer cross protectionagainst SE and SG infection.
Abstract
In the present study, we investigated the protection conferredby a live attenuated Salmonella enterica serovarTyphimurium (ST) strain against Salmonella Typhimurium,Salmonella Gallinarum (SG), and Salmonella Enteritidis(SE) infection in layer chickens. Birds were orally primed withthe attenuated ST strain at 7 days of age and then boostedat 4 weeks post prime immunization (PPI). Sequential monitoringof plasma IgG and mucosal secretory IgA (sIgA) levelsrevealed that inoculation with ST induced a significant antibodyresponse to antigens against ST, SE, and SG. Moreover, significant lymphoproliferative responses to the 3Salmonella serovars were observed in the immunized group. We also investigated protection against virulent ST, SE, andSG strain challenge. Upon virulent SG challenge, the immunizedgroup showed significantly reduced mortality comparedto the non-immunized group. The reduced persistenceof the virulent ST and SE challenge strains in the liver,spleen, and cecal tissues of the immunized group suggeststhat immunization with the attenuated ST strain may not onlyprotect against ST infection but can also confer cross protectionagainst SE and SG infection.
참고문헌 (45)
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