산란계에서 Chlorhexidine-inactivated Salmonella Enteritidis, S. Typhimurium 및 S. Gallinarum 3가 백신의 효능평가
Evaluation of the protective efficacy of trivalent Salmonella inactivated vaccine including Chlorhexidine-inactivated Salmonella Enteritidis, S. Typhimurium and S. Gallinarum in poultry
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Protective efficacy of trivalent Salmonella inactivated vaccine containing Chlorhexidine-inactivated S . Enterltidis (SE), S . Typhimurium (ST), and S . Gallinarum (SG) strains, was evaluated in this study. A total of 70 brown nick layers were divided into 7 groups, A to G, containing 10 hens per group. All hens in groups B to D were intramuscularly immunized with approximately 7×108 cells (3×108 cells of SE+1×108 cells of ST+3×108 cells of SG) of the trivalent vaccine in 0.5 mL of PBS. All chickens in groups E to G were injected with sterile PBS. All hens of groups B and E, groups C and F, and groups D and G were orally challenged with approximately 2 ×109 CFU of wild-type SE, ST, and SG, respectively. Serum IgG titers and CD3+CD4+ T-cells, and CD3+CD8+ T-cells levels of groups B to D significantly higher than those of group A. In addition, all animals in groups A to C, E and F showed no clinical symptoms and survived after the virulent challenges, whereas one chicken in group D died and all chickens in group G died following the challenge. The protection against wild-type SE and ST in liver, spleen, cecum, and cloaca of groups B and C chickens was significant effective as compared with those in groups E and F. These indicate that the trivalent inactivated vaccine can be an effective tool for prevention of Salmonella infections by inducing robustly protective immune responses and cellular immune response in chickens.
Abstract
Protective efficacy of trivalent Salmonella inactivated vaccine containing Chlorhexidine-inactivated S . Enterltidis (SE), S . Typhimurium (ST), and S . Gallinarum (SG) strains, was evaluated in this study. A total of 70 brown nick layers were divided into 7 groups, A to G, containing 10 hens per group. All hens in groups B to D were intramuscularly immunized with approximately 7×108 cells (3×108 cells of SE+1×108 cells of ST+3×108 cells of SG) of the trivalent vaccine in 0.5 mL of PBS. All chickens in groups E to G were injected with sterile PBS. All hens of groups B and E, groups C and F, and groups D and G were orally challenged with approximately 2 ×109 CFU of wild-type SE, ST, and SG, respectively. Serum IgG titers and CD3+CD4+ T-cells, and CD3+CD8+ T-cells levels of groups B to D significantly higher than those of group A. In addition, all animals in groups A to C, E and F showed no clinical symptoms and survived after the virulent challenges, whereas one chicken in group D died and all chickens in group G died following the challenge. The protection against wild-type SE and ST in liver, spleen, cecum, and cloaca of groups B and C chickens was significant effective as compared with those in groups E and F. These indicate that the trivalent inactivated vaccine can be an effective tool for prevention of Salmonella infections by inducing robustly protective immune responses and cellular immune response in chickens.
참고문헌 (47)
- Alvarez J, "Development of a multiplex PCR technique for detection and epidemiological typing of Salmonella in human clinical samples", J Clin Microbiol, 42, pp.1734~1738 (2004)
- Barbezange C, "Some safety aspects of Salmonella vaccines for poultry : in vivo study of the genetic stability of three Salmonella Typhimurium live vaccines", FEMS Microbiol Lett, 192, pp.101~106 (2000)
- Bouzoubaa K, "Feasibility of using proteins from Salmonella Gallinarum vs. 9R live vaccine for the prevention of fowl typhoid in chickens", Avian Dis, 33, pp.385~391 (1989)
- Brumme S, "Impact of Salmonella Typhimurium DT104 virulence factors invC and sseD on the onset, clinical course, colonization patterns and immune response of porcine salmonellosis", Vet Microbiol, 124, pp.274~285 (2007)
- Clifton-Hadley FA, "A laboratory study of an inactivated bivalent iron restricted Salmonella enterica serovars Enteritidis and Typhimurium dual vaccine against Typhimurium challenge in chickens", Vet Microbiol, 89, pp.167~179 (2002)
- Desin TS, "Salmonella vaccines in poultry : past, present and future", Expert Rev Vaccines, 12, pp.87~96 (2013)
- Feng P, "Increased sensitivity to Salmonella enterica serovar Typhimurium infection in mice undergoing withdrawal from morphine is associated with suppression of interleukin-12", Infect Immun, 73, pp.7953~7959 (2005)
- Galanis E, "Web-based surveillance and global Salmonella distribution, 2000-2002", Emerg Infect Dis, 12, pp.381~388 (2006)
- Garmory HS, "Salmonella vaccines for use in humans : present and future perspectives", FEMS Microbiol Rev, 26, pp.339~353 (2002)
- Gast RK, "Evaluation of the efficacy of oil-emulsion bacterins for reducing fecal shedding of Salmonella Enteritidis by laying hens", Avian Dis, 37, pp.1085~1091 (1993)
- Gast RK, "Evaluation of the efficacy of an oil-emulsion bacterin for protecting chickens against Salmonella Enteritidis", Avian Dis, 36, pp.992~999 (1992)
- Helms M, "International Salmonella Typhimurium DT104 infections, 1992-2001", Emerg Infect Dis, 11, pp.859~867 (2005)
- Hur J, "Evaluation of efficacy of a new live Salmonella Typhimurium vaccine candidate in a murine model", Comp Immunol Microbiol Infect Dis, 34, pp.171~177 (2011)
- 장혜정, "2021년 경남지역 Salmonella enterica serotype Enteritidis 원인 식중독의 분자역학적 특성 분석", 생명과학회지, 33(1), pp.56~63 (2023), 한국생명과학회[DOI]
- Jawale CV, "A novel approach for the generation of Salmonella Gallinarum ghosts and evaluation of their vaccine potential using a prime-booster immunization strategy", Vaccine, 32, pp.6776~6782 (2014)
- Jawale CV, "An immunogenic Salmonella ghost confers protection against internal organ colonization and egg contamination", Vet Immunol Immunopathol, 162(1-2), pp.41~50 (2014)
- Jawale CV, "Comparative evaluation of Salmonella Enteritidis ghost vaccines with a commercial vaccine for protection against internal egg contamination with Salmonella", Vaccine, 32, pp.5925~5930 (2014)
- Jawale CV, "Evaluation of immunogenicity and protective efficacy of adjuvanted Salmonella Typhimurium ghost vaccine against salmonellosis in chickens", Vet Q, 36, pp.130~136 (2016)
- Kang MS, "Differential identification of Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum based on polymorphic regions of glgC and speC genes", Vet microbiol, 147, pp.181~185 (2011)
- Kang ZW, "Genotypic and phenotypic diversity of Salmonella Enteritidis isolated from chickens and humans in Korea", J Vet Med Sci, 71, pp.1433~1438 (2009)
- Shukho Kim, "Genomic Relationship of Salmonella enterica Serovar Typhimurium DT104 Isolates from Korea and the United States", The Journal of Microbiology, 42(1), pp.14~19 (2004), 한국미생물학회
- 김석호, "사람에서 분리된 살모넬라의 국내 유행 혈청형과 해외 유입 혈청형, 2004~2005", Journal of Bacteriology and Virology, 36(2), pp.69~72 (2006), 대한미생물학회
- 김성재, "이유자돈에서 Salmonella Typhimurium 감염에 대한 박테리오파지의 방어 효능", 한국동물위생학회지, 37(1), pp.35~43 (2014), 한국동물위생학회
- Kim WK, "Protective efficacy of an inactivated Brucella abortus vaccine candidate lysed by GI24 against brucellosis in Korean black goats", Can J Vet Res, 83, pp.68~74 (2019)
- Lillehoj EP, "Vaccines against the avian enteropathogens Eimeria, Cryptosporidium and Salmonella", Anim Health Res Rev, 1, pp.47~65 (2000)
- Mailson ST, "Assessment of maternal immunity against Salmonella enterica serovar Heidelberg in progeny of broiler breeders vaccinated with different formulations of bacterins", Avian Pathol, 51, pp.197~205 (2022)
- Mastroeni P, "Salmonella : immune responses and vaccines", Vet J, 161, pp.132~164 (2001)
- McSorley SJ, "Characterization of CD4+ T cell responses during natural infection with Salmonella typhimurium", J Immunol, 164, pp.986~993 (2000)
- Ministry of Food and Drug Safety, Korea, "Food safety Korea, Food poisoning statistics", ~ (2022)
- Mittrücker HW, "Immune response to infection with Salmonella Typhimurium in mice", J Leukoc Biol, 67, pp.457~463 (2000)
- Mittrücker HW, "Characterization of the murine T-lymphocyte response to Salmonella enterica serovar Typhimurium infection", Infect Immun, 70, pp.199~203 (2002)
- Mittrücker HW, "Cutting edge : role of B lymphocytes in protective immunity against Salmonella Typhimurium infection", J Immunol, 164, pp.1648~1652 (2000)
- 문자영, "마우스에서 살모넬라 감염증 예방을 위한 신개념 Salmonella Typhimurium 불활화 사균체에 최적 adjuvant 선택을 위한 효능 비교 시험", 한국동물위생학회지, 43(2), pp.89~97 (2020), 한국동물위생학회[DOI]
- Muotiala A, "Protective immunity in mouse salmonellosis : comparison of smooth and rough live and killed vaccines", Microb Pathog, 6, pp.51~60 (1989)
- Nandre RM, "Immunogenicity of a Salmonella Enteritidis mutant as vaccinecandidate and its protective efficacy against salmonellosis in chickens", Vet Immunol Immunopathol, 144, pp.299~311 (2011)
- Nauciel C, "Role of CD4+ T cells and T-independent mechanisms in acquired resistance to Salmonella Typhimurium infection", J Immunol, 145, pp.1265~1269 (1990)
- Nicholas RA, "Detection of antibody to Salmonella Enteritidis and S Typhimurium in the yolk of hens' eggs", Vet Rec, 128, pp.98~100 (1991)
- Norimatsu M, "Live Salmonella enterica serovar Typhimurium (S. Typhimurium) elicit dendritic cell responses that differ from those induced by killed S. Typhimurium", Vet immunol Immunopathol, 98, pp.193~201 (2004)
- Pascual DW, "Fimbriated Salmonella enterica serovar Typhimurium abates initial inflammatory responses by macrophages", Infect immun, 70, pp.4273~4281 (2002)
- Roesler U, "Immunization of sows in an integrated pig-breeding herd using a homologous inactivated Salmonella vaccine decreases the prevalence of Salmonella Typhimurium infection in the offspring", J Vet Medi B Infect Dis Vet Public Health, 53, pp.224~228 (2006)
- Senevirathne A, "Immunization of chicken with flagellin adjuvanted Salmonella Enteritidis bacterial ghosts confers complete protection against chicken salmonellosis", Poult Sci, 100, pp.101205~ (2021)
- Senevirathne A, "Immunization of chickens with Salmonella Gallinarium ghosts expressing Salmonella Enteritidis NFliC-FimAC and CD40LC fusion antigen enhances cell-mediated immune responses and protects against wild-type challenges with both species", Dev Comp Immunol, 126, pp.104265~ (2022)
- Stenger S, "An antimicrobial activity of cytolytic T cells mediated by granulysin", Science, 282, pp.121~125 (1998)
- 원가연, "Protective efficacy and immune responses by homologous prime-booster immunizations of a novel inactivated Salmonella Gallinarum vaccine candidate", Clinical and Experimental Vaccine Research, 5(2), pp.148~158 (2016), 대한백신학회
- Gayeon Won, "A Salmonella Typhi ghost induced by the E gene of phage jX174 stimulates dendritic cells and efficiently activates the adaptive immune response", Journal of Veterinary Science, 19(4), pp.536~542 (2018), 대한수의학회[DOI]
- Woo YK, "Comparison of diseases resistance between white and brown layer lines to experimental infection of Salmonella Gallinarum", Korean J Vet Res, 38, pp.784~792 (1998)
- 양시용, "살모넬라 부재 계육 생산을 위한 위생관리", 한국가금학회지, 37(3), pp.289~295 (2010), 한국가금학회