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가금티푸스 예방을 위한 adjuvant로서 mastoparan V1을 사용한 포르말린-불활화 Salmonella Gallinarum 사균체 백신의 효능 평가

Protective efficacy of formalin-inactivated Salmonella Gallinarum whole cells vaccine using mastoparan V1 as adjuvant against fowl typhoid

김선민|이준우|김원경|배창환|Enkhsaikhan Ochirkhuyag|조영규|문자영|곽길환|방우영|허 진
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2019.12|한국동물위생학회지|제42권 제4호|pp. 257-264|DOI 10.7853/kjvs.2019.42.4.257

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ChickenVaccineFowl typhoidSalmonella GallinarumMastoparan V1
주제분류수의학

초록

Mastoparan V1 was used as adjuvant of formalin-inactivated Salmonella Gallinarum whole cells vaccine against fowl typhoid in a chicken model. The 75 brown nick chickens were equally divided into 5 groups, and all chickens of each group were immunized at 6 weeks of age (0 WPPI; weeks prime post immunization), and at 9 weeks of age (3 WPPI) (except group B). Group A chickens were intra-muscularly (IM) inoculated with 500 uL of sterile phosphate-buffered saline (PBS), and group B chick-ens were subcutaneously immunized with 0.2 ml containing 5×107 viable vaccine strain/bird. The chick-ens in groups C∼E were IM inoculated with approximately 3×109 cells/0.5 mL of formalin-inactivated the S. Gallinarum whole cells, approximately 3×109 cells/0.5 mL of formalin-inactivated the S. Gallinarum whole cells with mastoparan V1 as adjuvant, and 0.5 mL of PBS, respectively. S. Gallinarum outer membrane proteins-specific serum IgG titers were considerably higher in groups B∼D than in groups A and E. However, the levels of IFN- in groups B and D only than in groups A and E were sig-nificantly higher. Following oral challenge with virulent wild-type S. Gallinarum, no chicken in groups A (no challenge group) and B was dead, and only 30% of chickens in group D was dead. However, 70% of chickens in group C and all chickens in group E were dead after oral challenge. The results of this study demonstrated that IM immunization with approximately 3×109 of the formalin-inactivated S. Gallinarum whole cells containing mastoparan V1 induced robust antibody and cell-mediated immune responses in chickens. The whole cells also conferred protection against infection with wild-type S. Gallinarum.

Abstract

Mastoparan V1 was used as adjuvant of formalin-inactivated Salmonella Gallinarum whole cells vaccine against fowl typhoid in a chicken model. The 75 brown nick chickens were equally divided into 5 groups, and all chickens of each group were immunized at 6 weeks of age (0 WPPI; weeks prime post immunization), and at 9 weeks of age (3 WPPI) (except group B). Group A chickens were intra-muscularly (IM) inoculated with 500 uL of sterile phosphate-buffered saline (PBS), and group B chick-ens were subcutaneously immunized with 0.2 ml containing 5×107 viable vaccine strain/bird. The chick-ens in groups C∼E were IM inoculated with approximately 3×109 cells/0.5 mL of formalin-inactivated the S. Gallinarum whole cells, approximately 3×109 cells/0.5 mL of formalin-inactivated the S. Gallinarum whole cells with mastoparan V1 as adjuvant, and 0.5 mL of PBS, respectively. S. Gallinarum outer membrane proteins-specific serum IgG titers were considerably higher in groups B∼D than in groups A and E. However, the levels of IFN- in groups B and D only than in groups A and E were sig-nificantly higher. Following oral challenge with virulent wild-type S. Gallinarum, no chicken in groups A (no challenge group) and B was dead, and only 30% of chickens in group D was dead. However, 70% of chickens in group C and all chickens in group E were dead after oral challenge. The results of this study demonstrated that IM immunization with approximately 3×109 of the formalin-inactivated S. Gallinarum whole cells containing mastoparan V1 induced robust antibody and cell-mediated immune responses in chickens. The whole cells also conferred protection against infection with wild-type S. Gallinarum.

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