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Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration of Salmonella enterica Serovar Typhimurium Expressing Chicken Interleukin-18

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Interleukin-18 (IL-18) has been known to induce interferon-γ(IFN-γ) production and promote Th1 immunity. Although mammalian IL-18 has been characterized in great detail, the properties and application of chicken IL-18 remain largely uninvestigated as of yet. In this study, we evaluated the immunomodulatory properties of Salmonella enterica serovar Typhimurium expressing chicken interleukin-18 (chIL-18) on immune responses induced by avian influenza (AI) and Newcastle disease (ND) vaccines. After oral administration of S. enterica serovar Typhimurium expressing chIL-18,chickens were vaccinated intramuscularly with the recommended dose of either inactivated AI H9N2 vaccine or ND (B1 strain) vaccine. Chickens receiving a primary vaccination were boosted using the same protocol 7 days later. Humoral and cell-mediated immune responses were evaluated in terms of HI antibody titers and proliferation and mRNA expression of IFN-γ and IL-4 of peripheral blood mononuclear cells (PBMC) in response to specific antigen stimulation. According to our results, oral administration of S. enterica serovar Typhimurium expressing chIL-18 induced enhanced humoral and Th1-biased cell-mediated immunity against AI and ND vaccines, compared to that of chickens received S. enterica serovar Typhimurium harboring empty vector. Therefore, we conclude that our proposed vaccination regimen using inactivated AI and ND viruses along with oral administration of S. enterica serovar Typhimurium expressing chIL-18 may provide a novel approach in protecting chicken from currently circulating AI and ND virus strains.

Abstract

Interleukin-18 (IL-18) has been known to induce interferon-γ(IFN-γ) production and promote Th1 immunity. Although mammalian IL-18 has been characterized in great detail, the properties and application of chicken IL-18 remain largely uninvestigated as of yet. In this study, we evaluated the immunomodulatory properties of Salmonella enterica serovar Typhimurium expressing chicken interleukin-18 (chIL-18) on immune responses induced by avian influenza (AI) and Newcastle disease (ND) vaccines. After oral administration of S. enterica serovar Typhimurium expressing chIL-18,chickens were vaccinated intramuscularly with the recommended dose of either inactivated AI H9N2 vaccine or ND (B1 strain) vaccine. Chickens receiving a primary vaccination were boosted using the same protocol 7 days later. Humoral and cell-mediated immune responses were evaluated in terms of HI antibody titers and proliferation and mRNA expression of IFN-γ and IL-4 of peripheral blood mononuclear cells (PBMC) in response to specific antigen stimulation. According to our results, oral administration of S. enterica serovar Typhimurium expressing chIL-18 induced enhanced humoral and Th1-biased cell-mediated immunity against AI and ND vaccines, compared to that of chickens received S. enterica serovar Typhimurium harboring empty vector. Therefore, we conclude that our proposed vaccination regimen using inactivated AI and ND viruses along with oral administration of S. enterica serovar Typhimurium expressing chIL-18 may provide a novel approach in protecting chicken from currently circulating AI and ND virus strains.

참고문헌 (28)

  1. Fouchier, R. A., "Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls", J. Virol, 79, pp.2814~2822 (2005)
  2. Alexander, D. J., "A review of avian influenza in different bird species", Vet. Microbiol, 74, pp.3~13 (2000)
  3. Butt, K. M., "Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003", J. Clin. Microbiol, 43, pp.5760~5767 (2005)
  4. Li, K. S., "Characterization of H9 subtype influenza viruses from the ducks of southern China: a candidate for the next influenza pandemic in humans?", J. Virol, 77, pp.6988~6994 (2003)
  5. Alexander, D. J, "Newcastle disease, other avian Paramyxoviruses, and Pneumovirusinfections, In Diseases of poultry", pp.63~87 (2003), IowaState Press
  6. Senne, D. A., "Control of Newcastle disease by vaccination", Dev. Biol. (Basel), 119, pp.165~170 (2004)
  7. van Boven, M., "Herd immunity to Newcastle disease virus in poultry by vaccination", Avian. Pathol, 37, pp.1~5 (2008)
  8. Kapczynski, D. R., "Protection of chickens against overt clinical disease and determination of viral shedding following vaccination with commercially available Newcastle disease virus vaccines upon challenge with highly virulent virus from the California 2002 exotic Newcastle disease outbreak", Vaccine, 23, pp.3424~3433 (2005)
  9. van Oirschot, J. T., "Diva vaccines that reduce virus transmission", J. Biotechnol, 73, pp.195~205 (1999)
  10. Kaiser, P., "Advances in avian immunology--prospects for disease control: a review", Avian. Pathol, 39, pp.309~324 (2010)
  11. Akira, S., "The role of IL-18 in innate immunity", Curr. Opin. Immunol, 12, pp.59~63 (2000)
  12. Rahman, M. M., "Enhancement of Th1-biased protective immunity against avian influenza H9N2 virus via oral co-administration of attenuated Salmonella enterica serovar Typhimurium expressing chicken interferon-α and interleukin-18 along with an inactivated vaccine", BMC Vet. Res, 8, pp.105~ (2012)
  13. Rahman, M. M., "Oral co-administration of live attenuated Salmonella enterica serovar Typhimurium expressing chicken interferon-α and interleukin-18 enhances the alleviation of clinical signs caused by respiratory infection with avian influenza virus H9N2", Vet. Microbiol, 157, pp.448~455 (2012)
  14. Hung, L. H., "Adjuvant effects of chicken interleukin-18 in avian Newcastle disease vaccine", Vaccine, 28, pp.1148~1155 (2010)
  15. Lim, K. L., "Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses", BMC Vet. Res, 8, pp.132~ (2012)
  16. Ding, A. H., "Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production", J. Immunol, 141, pp.2407~2412 (1988)
  17. Rahman, M. M., "Oral administration of live attenuated Salmonella enterica serovar Typhimurium expressing chicken interferon-α alleviates clinical signs caused by respiratory infection with avian influenza virus H9N2", Vet. Microbiol, 154, pp.140~151 (2011)
  18. Pollock, K. G., "Interleukin-18 plays a role in both the alum-induced T helper 2 response and the T helper 1 response induced by alum-adsorbed interleukin-12", Immunology, 108, pp.137~143 (2003)
  19. Schneider, K., "cDNA cloning of biologically active chicken interleukin-18", J. Interferon. Cytokine. Res, 20, pp.879~883 (2000)
  20. Göbel, T. W., "IL-18 stimulates the proliferation and IFN-gamma release of CD4+ T cells in the chicken: conservation of a Th1-like system in a nonmammalian species", J. Immunol, 171, pp.1809~1815 (2003)
  21. Degen, W. G., "Potentiation of humoral immune responses to vaccine antigens by recombinant chicken IL-18 (rChIL-18)", Vaccine, 23, pp.4212~4218 (2005)
  22. Su, B. S., "Potentiation of cell-mediated immune responses against recombinant HN protein of Newcastle disease virus by recombinant chicken IL-18. Vet", Immunol. Immunopathol, 141, pp.283~292 (2011)
  23. Kim, S. J., "Co-administration of live attenuated Salmonella enterica serovar Typhimurium expressing swine interleukin-18 and interferon-α provides enhanced Th1-biased protective immunity against inactivated vaccine of pseudorabies virus", Microbiol. Immunol, 56, pp.529~540 (2012)
  24. Lee, B. M., "Enhanced protection against infection with transmissible gastroenteritis virus in piglets by oral co-administration of live attenuated Salmonella enterica serovar Typhimurium expressing swine interferon-α and interleukin-18. Comp", Immunol. Microbiol. Infect. Dis, 34, pp.369~380 (2011)
  25. Beal, R. K., "Clearance of enteric Salmonella enterica serovar Typhimurium in chickens is independent of B-cell function", Infect. Immun, 74, pp.1442~1444 (2006)
  26. Beal, R. K., "Age at primary infection with Salmonella enterica serovar Typhimurium in the chicken influences persistence of infection and subsequent immunity to re-challenge", Vet. Immunol. Immunopathol, 100, pp.151~164 (2004)
  27. Steidler, L., "Biological containment of genetically modified Lactococcus lactis for intestinal delivery of human interleukin-10", Nat. Biotechnol, 21, pp.785~789 (2003)
  28. Rupa, P., "Expression of bioactive porcine interferon-gamma by recombinant Lactococcus lactis", Vet. Microbiol, 129, pp.197~202 (2008)

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