Construction and Immunogenicity of Recombinant Swinepox Virus Expressing Outer Membrane Protein L of Salmonella
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Salmonella spp. are gram-negative flagellated bacteria that cause a variety of diseases in humans and animals, ranging from mild gastroenteritis to severe systemic infection. To explore development of a potent vaccine against Salmonella infections, the gene encoding outer membrane protein L (ompL) was inserted into the swinepox virus (SPV) genome by homologous recombination. PCR, western blot, and immunofluorescence assays were used to verify the recombinant swinepox virus rSPV-OmpL. The immune responses and protection efficacy of rSPV-OmpL were assessed in a mouse model. Forty mice were assigned to four groups, which were immunized with rSPV-OmpL, inactive Salmonella (positive control), wildtype SPV (wtSPV; negative control), or PBS (challenge control), respectively. The OmpLspecific antibody in the rSPV-OmpL-immunized group increased dramatically and continuously over time post-vaccination, and was present at a significantly higher level than in the positive control group (p < 0.05). The concentrations of IFN-γ and IL-4, which represent Th1-type and Th2-type cytokine responses, were significantly higher (p < 0.05) in the rSPVOmpL- vaccinated group than in the other three groups. After intraperitoneal challenge with a lethal dose of Salmonella typhimurium CVCC542, eight out of ten mice in the rSPV-OmpLvaccinated group were protected, whereas all the mice in the negative control and challenge control groups died within 3 days. Passive immune protection assays showed that hyperimmune sera against OmpL could provide mice with effective protection against challenge from S. typhimurium. The recombinant swinepox virus rSPV-OmpL might serve as a promising vaccine against Salmonella infection.
Abstract
Salmonella spp. are gram-negative flagellated bacteria that cause a variety of diseases in humans and animals, ranging from mild gastroenteritis to severe systemic infection. To explore development of a potent vaccine against Salmonella infections, the gene encoding outer membrane protein L (ompL) was inserted into the swinepox virus (SPV) genome by homologous recombination. PCR, western blot, and immunofluorescence assays were used to verify the recombinant swinepox virus rSPV-OmpL. The immune responses and protection efficacy of rSPV-OmpL were assessed in a mouse model. Forty mice were assigned to four groups, which were immunized with rSPV-OmpL, inactive Salmonella (positive control), wildtype SPV (wtSPV; negative control), or PBS (challenge control), respectively. The OmpLspecific antibody in the rSPV-OmpL-immunized group increased dramatically and continuously over time post-vaccination, and was present at a significantly higher level than in the positive control group (p < 0.05). The concentrations of IFN-γ and IL-4, which represent Th1-type and Th2-type cytokine responses, were significantly higher (p < 0.05) in the rSPVOmpL- vaccinated group than in the other three groups. After intraperitoneal challenge with a lethal dose of Salmonella typhimurium CVCC542, eight out of ten mice in the rSPV-OmpLvaccinated group were protected, whereas all the mice in the negative control and challenge control groups died within 3 days. Passive immune protection assays showed that hyperimmune sera against OmpL could provide mice with effective protection against challenge from S. typhimurium. The recombinant swinepox virus rSPV-OmpL might serve as a promising vaccine against Salmonella infection.
참고문헌 (35)
- Abd El Ghany M, "Candidate live, attenuated Salmonella enterica serotype Typhimurium vaccines with reduced fecal shedding are immunogenic and effective oral vaccines", Infect. Immun, 75, pp.1835~1842 (2007)
- Afonso CL, "The genome of swinepox virus", J. Virol, 76, pp.783~790 (2002)
- Barcena J, "Recombinant swinepox virus expressing beta-galactosidase: investigation of viral host range and gene expression levels in cell culture", Virology, 243, pp.396~405 (1998)
- Benschop J, "Temp oral and longitudinal analysis of Danish Swine Salmonellosis Control Programme data: implications for surveillance", Epidemiol. Infect, 136, pp.1511~1520 (2008)
- Cao J, "Enhanced protection against pneumococcal infection elicited by immunization with the combination of PspA, PspC, and ClpP", Vaccine, 25, pp.4996~5005 (2007)
- DasSarma P, "Haloarchaeal gas vesicle nanoparticles displaying Salmonella SopB antigen reduce bacterial burden when administered with live attenuated bacteria", Vaccine, 32, pp.4543~4549 (2014)
- De Ridder L, "Use of a live attenuated Salmonella enterica serovar Typhimurium vaccine on farrow-to-finish pig farms", Vet. J, 202, pp.303~308 (2014)
- Draper SJ, "Viruses as vaccine vectors for infectious diseases and cancer", Nat. Rev. Microbiol, 8, pp.62~73 (2010)
- Ghosh S, "An adhesion p rotein of Salmonella enterica serovar Typhi is required for pathogenesis and potential target for vaccine development", Proc. Natl. Acad. Sci. USA, 108, pp.3348~3353 (2011)
- Hahn J, "Construction of recombinant swinepox viruses and expression of the classical swine fever virus E2 protein", J. Virol. Methods, 93, pp.49~56 (2001)
- Hamid N, "Characterization of an outer membrane protein of Salmonella enterica serovar Typhimurium that confers protection against typhoid", Clin. Vaccine Immunol, 15, pp.1461~1471 (2008)
- Hong EH, "Intratumoral injection of attenuated Salmonella vaccine can induce tumor microenvironmental shift from immune suppressive to immunogenic", Vaccine, 31, pp.1377~1384 (2013)
- Lin HX, "A novel p vaccine against Streptococcus equi ss . zooepidemicus infections: the recombinant swinepox virus expressing Mlike protein", Vaccine, 29, pp.7027~7034 (2011)
- Lin HX, "A novel vaccine against porcine circovirus type 2 (PCV2) and Streptococcus equi ssp . zooepidemicus (SEZ) co-infection", Vet. Microbiol, 171, pp.198~205 (2014)
- Majowicz SE, "The global burden of nontyphoidal Salmonella gastroenteritis", Clin. Infect. Dis, 50, pp.882~889 (2010)
- Mathur R, "A mouse model of Salmonella typhi infection", Cell, 151, pp.590~602 (2012)
- Moorkamp L, "Swinepox - skin disease with sporadic occurrence", Dtsch. Tierarztl. Wochenschr, 115, pp.162~166 (2008)
- Murakami S, "Occurrence of swine salmonellosis in postweaning multisystemic wasting syndrome (PMWS) affected pigs concurrently infected with porcine reproduction and respiratory syndrome virus (PRRSV)", J. Vet. Med. Sci, 68, pp.387~391 (2006)
- Nanton MR, "Cutting edge: B cells are essential for protective immunity against Salmonella independent of antibody secretion", J. Immunol, 189, pp.5503~5507 (2012)
- Okamura M, "Immunization with outer membrane protein A from Salmonella enterica serovar Enteritidis induces humoral immune response but no protection against homologous challenge in chickens", Poult. Sci, 91, pp.2444~2449 (2012)
- Pantaleo G, "Poxvirus vector-based HIV vaccines", Curr. Opin. HIV AIDS, 5, pp.391~396 (2010)
- Paoletti E, "Applications of pox virus vectors to vaccination: an update", Proc. Natl. Acad. Sci. USA, 93, pp.11349~11353 (1996)
- Penha Filho RA, "Humoral and cellular immune response generated by different vaccine programs before and after Salmonella Enteritidis challenge in chickens", Vaccine, 30, pp.7637~7643 (2012)
- Poulet H, "Development and registration of recombinant veterinary vaccines. The example of the canarypox vector platform", Vaccine, 25, pp.5606~5612 (2007)
- Ruggeri J, "Parenteral administration of attenuated Salmonella Typhimurium DeltaznuABC is protective against salmonellosis in piglets", Vaccine, 32, pp.4032~4038 (2014)
- Song J, "Structure and function of the Salmonella Typhi chimaeric A(2)B(5) typhoid toxin", Nature, 499, pp.350~354 (2013)
- Strugnell RA, "Salmonella vaccines: lessons from the mouse model or bad teaching", Curr. Opin. Microbiol, 17, pp.99~105 (2014)
- Sundara Baalaji N, "Functional assay of Salmonella typhi OmpC using reconstituted large unilamellar vesicles: a general method for characterization of outer membrane proteins", Biochimie, 88, pp.1419~1424 (2006)
- Trip athy DN, "Swinep ox virus as a vaccine vector for swine pathogens", Adv. Vet. Med, 41, pp.463~480 (1999)
- Winslow BJ, "Replication and expression of a swinepox virus vector delivering feline leukemia virus Gag and Env to cell lines of swine and feline origin", Virus Res, 98, pp.1~15 (2003)
- Winslow BJ, "Feline B7.1 and B7.2 proteins produced from swinepox virus vectors are natively processed and biologically active: potential for use as nonchemical adjuvants", Vet. Microbiol, 111, pp.1~13 (2005)
- Yamanouchi K, "New approaches to the development of virus vaccines for veterinary use", Rev. Sci. Tech, 17, pp.641~653 (1998)
- Yang TC, "Screening of the Salmonella paratyphi A CMCC 50973 strain outer membrane proteins for the identification of potential vaccine targets", Mol. Med. Rep, 5, pp.78~83 (2012)
- Yang Y, "Identification and characterization of OmpL as a potential vaccine candidate for immune-protection against salmonellosis in mice", Vaccine, 31, pp.2930~2936 (2013)
- Zhang Y, "Projected burden of disease for Salmonella infection due to increased temperature in Australian temperate and subtropical regions", Environ. Int, 44, pp.26~30 (2012)