본문 바로가기
KERD
논문KCI 등재

桑葉 함유 한약복합제 추출물의 抗肥滿효과 연구

The Study on Anti-obesity Effects of Mulberry Leaves Contained Herbal Mixture

박은정|강경하|박종익
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2013.11|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제27권 제4호|pp. 17-30

2

조회

0

다운로드

2

인용

0

참고문헌

키워드

ObesityMulberry leavesSerum lipidAdipocyte differentiation3T3-L1 cellPPAR γC/EBP α
주제분류한의학

초록

Objectives This experimental study was designed to investigate the effects of Mulberry leaves contained herbal mixture (MLHM) on body weight, serum lipid level and adipocyte differentiation in high fat diet-fed obese mice. Methods Four-week old mice (wild-type C57/BL6) were used for all experiments. Cells were incubated with MLHM at the indicated concentration (0.04-4mg/㎖) for 24h, and growth rate was assessed by MTT ((3-(4,5-Dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide) assay. 3T3-L1 preadipocytes were incubated in DMEM for 2 days with the indicated concentrations of MLHM, and on Day 6, the cells were fixed and the cellular lipid contents were assessed by Oil-Red-O staining. The expression of peroxisome proliferator-activated receptor γ (PPAR γ) and cytidine-cytidine-adenosine-adenosinethymine (CCAAT)/enhancer-binding proteins α (C/EBP α) as adipocyte-specific proteins were determined by real time RT-PCR and western blotting. In addition, body weight gain and serum lipid levels were measured in the mice with obesity induced by the high fat-diet for four weeks. Results Though MLHM did not show toxicity even at the concentration of 4㎎/ml, MLHM significantly inhibited the differentiation of 3T3-L1 preadipocites in a dose-dependent manner. Also, MLHM significantly reduced the expressions of PPAR γ and C/EBP α in a dose-dependent manner. Furthermore, MLHM significantly reduced body weight gain and LDL-cholesterol contents in high fat diet-fed obese mice. Conclusions These results demonstrate that MLHM exerts anti-obesity effect in 3T3-L1 cells and mice with obesity by high-fat diet.

Abstract

Objectives This experimental study was designed to investigate the effects of Mulberry leaves contained herbal mixture (MLHM) on body weight, serum lipid level and adipocyte differentiation in high fat diet-fed obese mice. Methods Four-week old mice (wild-type C57/BL6) were used for all experiments. Cells were incubated with MLHM at the indicated concentration (0.04-4mg/㎖) for 24h, and growth rate was assessed by MTT ((3-(4,5-Dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide) assay. 3T3-L1 preadipocytes were incubated in DMEM for 2 days with the indicated concentrations of MLHM, and on Day 6, the cells were fixed and the cellular lipid contents were assessed by Oil-Red-O staining. The expression of peroxisome proliferator-activated receptor γ (PPAR γ) and cytidine-cytidine-adenosine-adenosinethymine (CCAAT)/enhancer-binding proteins α (C/EBP α) as adipocyte-specific proteins were determined by real time RT-PCR and western blotting. In addition, body weight gain and serum lipid levels were measured in the mice with obesity induced by the high fat-diet for four weeks. Results Though MLHM did not show toxicity even at the concentration of 4㎎/ml, MLHM significantly inhibited the differentiation of 3T3-L1 preadipocites in a dose-dependent manner. Also, MLHM significantly reduced the expressions of PPAR γ and C/EBP α in a dose-dependent manner. Furthermore, MLHM significantly reduced body weight gain and LDL-cholesterol contents in high fat diet-fed obese mice. Conclusions These results demonstrate that MLHM exerts anti-obesity effect in 3T3-L1 cells and mice with obesity by high-fat diet.

참고문헌 (58)

  1. Department of Internal Medicine, Seoul National University College of Medicine, "Internal Medicine", pp.852~862 (1996), Koonja publishing inc
  2. Georgy A Bray, "Office management of obesity", pp.21~114 (2005), Hanmi medical Pub
  3. Jung SH, "Research study about Obesity-related information acquisition method", J Oreintal Rehab Med, 8(2), pp.1~15 (1998)
  4. Kim TH, "Obesity and tatty Liver disease", Korean Journal of Medicine, 68(2), pp.347~349 (2005)
  5. Ministry of Health and Welfare, "Korea National Health and Nutrition Examination Survey (KNHANES V-1)" (2010), Korea Health Statistics
  6. 오경원, "한국 소아청소년의 비만 유병률 추이: 1997년과 2005년 비교", Korean Journal of Pediatrics, 51(9), pp.950~955 (2008), 대한소아과학회
  7. Peter GK, "Obesity as a medical problem", Nature, 404(6778), pp.635~643 (2000)
  8. "TCM assessment study, In TCM Sympton differentiation Diagnostics", pp.43~ (1987), People's Health Publishing House
  9. Ko SC, "The Study about the Comparison of Oriental-Western Medicine on Childhood Obesity", Journal Korean Oriental Pediatrics, 3(1), pp.57~61 (1989)
  10. Obesity Study Group of Korean Primary Care Association, "Theory and Practice of Obesity Studies", pp.267~ (1996), hankookbook
  11. Park YW, "Evidence-based prescriptions of functional foods fot weight control", Korean J Fam Med, 24(5), pp.409~415 (2003)
  12. Park CY, "The Update of Anti-Obesity Drug", Kyunghee medical book, 18(2), pp.77~85 (2002)
  13. The korean academy of oriental rehabilitation medicine, "Oriental Rehabilitation Medicine", pp.349~396 (2005), Koonja publishing inc
  14. Jung SH, "Antidiabetic effect and mechanism of Mori folium on streoptozotocin-induced diabetic mouse", East-west pharmaceutical research institute college of pharmacy kyunghee university, 28, pp.87~99 (2000)
  15. Kim MS, "Blood glucose lowering effects of Mulberry leaves and silkworm extracts on mice fed with high-carbohydrate diet", The Korean journal of nutrition, 31, pp.117~125 (1998)
  16. Myo CS, "coloured medicinal plant of Korea" (1989), Academic Co
  17. 전영준, "산약의 HeLa cell 분화에 미치는 영향과 항산화효과에 대한연구", 대한한방부인과학회지, 20(2), pp.139~154 (2007), 대한한방부인과학회
  18. 남주영, "安胎에 활용되는 山藥의 신경세포주에 대한 안전성 및 항산화효과에 대한 연구", 대한한방부인과학회지, 19(4), pp.61~76 (2006), 대한한방부인과학회
  19. McAnuff MA, "Hypoglycemic effects of steroidal sapogenins isolated from jamaican bitter yam, Dioscorea poygonoides", Food Chem. Toxicol, 43, pp.1667~1672 (2005)
  20. Kwon CS, "Anti-obesity effect of Dioscorea nipponica Makino with lipasseinhibitory activity in rodents", Biosci. Biotechnol. Biochem, 67, pp.1451~1456 (2003)
  21. Chen HL, "Effects of Taiwanese yam (Dioscoreajaponica Thunb var. pseudojaponica Yamamoto) on upper gut function and lipid metabolism in Balb", c mice, 2003, pp.19, 646~651
  22. Seo KI, "Antimicrobial and antioxidative activities of Corni fructus extracts", Journal of the Korean society of post-harvest science & technology of agricultural products, 6(1), pp.99~103 (1999)
  23. 김옥경, "산수유 추출물이 Streptozotocin으로 유발된 흰쥐의 항당뇨 및 항산화 작용에 미치는 효과", 韓國油化學會誌, 22(2), pp.157~167 (2005), 한국유화학회
  24. Sagi-Eisenberg R, "Histamine release induced by histone and phorbol ester from rat peritoneal mast cells", Eur J Pharmacol, 113, pp.11~17 (1985)
  25. 김성진, "지각 약침액의 항산화 및 항염증 효과에 관한 연구", Korean Journal of Acupuncture, 27(2), pp.13~24 (2010), 경락경혈학회
  26. Seoul National University College of Medicine, "Endocrinology jeonjeongpan", pp.425~495 (2006), Seoul National University Press
  27. Min HK, "Clinical Endocrinology", pp.476~486 (1990), Korea medical book
  28. Gordon Edlin, "Health and Wellness", pp.128~ (2010), 도서출판 영문출판사
  29. Kim YS, "Causes and classification of obesity", Kyunghee medicalbook, 11(3-4), pp.244~ (1995)
  30. Annual report on the cause of death statistics, "Statistics Korea", ~ (2000)
  31. Lee BK, "Symptom differentiation treatment", pp.77~ (1991), Seongbosa
  32. Korean Intellectual Property Office, , ~ (2008)
  33. Shin MK, "Clinical Herbology", pp.171~531 (1986), Yeonglimsa
  34. Kimura T, "Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of postprandial blood glucose in humans", J Agric Food Chem, 55(14), pp.5869~5874 (2007)
  35. 박지민, "Postprandial hypoglycemic effect of mulberry leaf in Goto-Kakizaki rats and counterpart control Wistar rats", Nutrition Research and Practice, 3(4), pp.272~278 (2009), 한국영양학회
  36. Oh KS, "Melanin-concentrating hormone-1 receptor antagonism and anti-obesity effects of ethanolic extract from Morus alba leaves in diet-induced obese mice", J Ethnopharmacol, 122(2), pp.216~220 (2009)
  37. Hsu JH, "Dioscorea as the principal herb of Die-Huang-Wan, a widely used herbal mixture in China, for improvement of insulin resistance in fructose-rich chow-fed rats", J Ethnopharmacol, 112(3), pp.577~584 (2007)
  38. Kim SW, "The antioxidant activity of dioscoreae rhizoma on GC-1 spg cell", pp.2006
  39. Yamabe N, "Identification of antidiabetic effect of iridoid glycosides and low molecular weight polyphenol fractions of Corni Fructus, a constituent of Hachimi-jio-gan, in streptozotocininduced diabetic rats", Biol Pharm Bull, 30(7), pp.1289~1296 (2007)
  40. Elzbieta M, Kurowska, "Hypolipidemic Effects and Absorption of Citrus Polymethoxylated Flavones in Hamsters with Diet-Induced Hypercholesterolemia", J. Agric. Food Chem, 52, pp.2879~2886 (2004)
  41. Jung UJ, "The Hypoglycemic Effects of Hesperidin and Naringin Are Partly Mediated by Hepatic Glucose-Regulating Enzymes in C57BL", KsJdb, J Nutr, pp.2004, 134[DOI]
  42. Jung UJ, "Effect of citrus flavonoids on lipid metabolism and glucose-regulating enzyme mRNA levels in type-2 diabetic mice", The International Journal of Biochemistry & Cell Biolog, 38(7), pp.1134~1145 (2006)
  43. MacDougald OA, "Regulated expression of the obese gene product(leptin)in white adipose tissue and 3T3-L1 adipocytes", Preceedings of the National Academy of Sciences, 92(20), pp.9034~9037 (1995)
  44. Chen, Yu-Kuo, "Effects of green tea polyphenol(-)-Epigallocatechin-3-gallate on newly developed high-fat", Western-style dietinduced obesity and metabolic syndrome in mice, 2011, pp.59(21), 11862~11871
  45. James M, Ntambi, "Adipocyte differentiation and gene expression", J Nutr, 130, pp.3122~3126 (2000)
  46. Rosen ED, "Transcriptional regulation of adipogenesis", Genes & Dev, 14, pp.1293~1307 (2000)
  47. Jeon T, "Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells", Life Sci, 75, pp.3195~3203 (2004)
  48. Cowherd RM, "Molecular regulation of adipocyte differentiation", Semin Cell Dev Biol, 10, pp.3~10 (1999)
  49. Cornelius P, "Regulation of adipocyte development", Annu Rev Nutr, 14, pp.99~129 (1994)
  50. Kim SH, "Vitisin A inhibits adipocyte differentiation through cell cycle arrest in 3T3-L1 cells", Biochem Biophys Res Commun, 372, pp.108~113 (2008)
  51. Kopelman PG, "Obesity as a medical problem", Nature, 404, pp.635~643 (2000)
  52. Camp HS, "Adipogenesis and fat-cell function in obesity and diabetes", Trends in Molecular Medicine, 8(9), pp.442~447 (2002)
  53. Rosen ED, "Molecular regulation of adipogenesis", Annu Rev Cell Dev Biol, 16, pp.145~171 (2000)
  54. Hwang CS, "Adipocyte differentiation and leptin expression", Annual review of cell and developmental biology, 13, pp.231~259 (1997)
  55. Plaa GL, "Detection and evaluation of chemically induced liver injury, In Principle and Methods of Toxicology", pp.839~870 (1994), Taylor & Francis
  56. Gordon T, "Lipoprotein, cardiovascular disease and death, the Framingham study", Arch Inter Med, 14, pp.1128~1135 (1981)
  57. Applebaum BD, "Down-regulation of the low density lipoprotein receptor by dietary cholesterol", Am J Clin Nurt, 39, pp.360~367 (1984)
  58. Potter SM, "Depression of plasma cholesterol in men by consumption of baked products containing soy protein", Am J Clin Nutr, 58, pp.501~506 (1993)

이 간행물의 다른 논문 (11건)

蔘出健脾湯加味方을 이용한 食癎 환아 치험 2례에 대한 증례보고김장현, 김은진, 민상연pp. 1-9주의력결핍·과잉행동장애의 침치료에 대한 최신 연구동향- 2011-2013년 중국 논문 중심으로 -강문수, 김락형, 김우경pp. 10-16소수관절형 소아기 류마티스관절염의 항류마티스제 감량과한약투여 병행을 통한 치료사례이윤주, 유창길pp. 31-38볏집 (稻草)이 DNCB로 유발된 생쥐의 알레르기성 접촉성 피부염에 미치는 영향박젬마, 채중원pp. 39-49소화기계 문제로 내원한 소아에게 한약투여가 성장에 미치는 영향고민정, 백정한, 김세영pp. 50-56
원문 보기