상엽 추출물의 항산화 활성 및 3T3-L1 세포에서 지방축적 억제 효과
Anti-oxidant and Anti-obese Effects of Mulberry (Morus alba L.) Leaf Extract in 3T3-L1 Cells
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Recently, numerous studies reported that excessive generation of ROS stimulated expression of adipogenesis transcription factors, such as C/EBPα, β, and PPARγ in 3T3-L1 cells. Therefore, effective scavenging of ROS could be regarded as strategy for anti-adipogenic effect through suppression of adipogenesis transcription factors. In this study, we examined the anti-oxidant and anti-adipogenic effects of mulberry (Morus alba L.) leaf extract (MLE). MLE was prepared by boiling water extraction method at 121°C for 15 min. DPPH radical scavenging activity of MLE was significantly increased to 3.48%, 8.28%, 16.63%, and 25.84% between 100 and 1,000 μg/mL. The MLE treatment for 24 h did not affect to the cell viability of confluent 3T3-L1 preadipocytes at concnetrations of 10, 50, 100, 200, 400, and 800 μg/mL. Thus, nontoxic concentration rages of MLE were treated during adipogenesis period (Day -2 to 6) from preadipocytes into adipocytes in 3T3-L1 cells. Intracellular lipid accumulation in MLE-treated 3T3-L1 adipocytes (Day 6) were quantitatively judged by ORO staining and a number of lipid droplet and size were observed using microscopy. Inhibitory activity of lipid accumulation by MLE treatment in 3T3-L1 adipocytes was significantly increased 19.52%, 16.98%, and 32.48% at 200, 400, and 800 μg/mL, respectively. The size and a number of lipid droplet in 3T3-L1 adipocytes were observed to dose-dependently decrease by MLE treatment. The mRNA expression levels of C/EBPα, β and PPARγ were decreased at 10 and 100 μg/mL in MLE-treated 3T3-L1 adipocytes compared with adipocytes. These results showed that antioxidant activity of MLE was contributed to anti-obesity effect by inhibition of intracellular lipid accumulation through suppression of adipogenesis transcription factors, such as C/EBPα, β, and PPARγ. Therefore, MLE is expected to utilize as anti-oxidant and anti-obesity cosmetic material.
Abstract
Recently, numerous studies reported that excessive generation of ROS stimulated expression of adipogenesis transcription factors, such as C/EBPα, β, and PPARγ in 3T3-L1 cells. Therefore, effective scavenging of ROS could be regarded as strategy for anti-adipogenic effect through suppression of adipogenesis transcription factors. In this study, we examined the anti-oxidant and anti-adipogenic effects of mulberry (Morus alba L.) leaf extract (MLE). MLE was prepared by boiling water extraction method at 121°C for 15 min. DPPH radical scavenging activity of MLE was significantly increased to 3.48%, 8.28%, 16.63%, and 25.84% between 100 and 1,000 μg/mL. The MLE treatment for 24 h did not affect to the cell viability of confluent 3T3-L1 preadipocytes at concnetrations of 10, 50, 100, 200, 400, and 800 μg/mL. Thus, nontoxic concentration rages of MLE were treated during adipogenesis period (Day -2 to 6) from preadipocytes into adipocytes in 3T3-L1 cells. Intracellular lipid accumulation in MLE-treated 3T3-L1 adipocytes (Day 6) were quantitatively judged by ORO staining and a number of lipid droplet and size were observed using microscopy. Inhibitory activity of lipid accumulation by MLE treatment in 3T3-L1 adipocytes was significantly increased 19.52%, 16.98%, and 32.48% at 200, 400, and 800 μg/mL, respectively. The size and a number of lipid droplet in 3T3-L1 adipocytes were observed to dose-dependently decrease by MLE treatment. The mRNA expression levels of C/EBPα, β and PPARγ were decreased at 10 and 100 μg/mL in MLE-treated 3T3-L1 adipocytes compared with adipocytes. These results showed that antioxidant activity of MLE was contributed to anti-obesity effect by inhibition of intracellular lipid accumulation through suppression of adipogenesis transcription factors, such as C/EBPα, β, and PPARγ. Therefore, MLE is expected to utilize as anti-oxidant and anti-obesity cosmetic material.
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