Pear pomace water extract inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes
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초록
Obesity occurs when a person’s calorie intake exceeds the amount of energy burns, which may lead to pathologic growth of adipocytes andthe accumulation of fat in the tissues. In this study, the effect and mechanism of pear pomace extracts on 3T3-L1 adipocyte differentiation andapoptosis of mature adipocytes were investigated. The effects of pear pomace extract on cell viability and the anti-adipogenic and proapoptoticeffects were investigated via MTT assay, Oil red O staining, western blot analysis and apoptosis assay. 3T3-L1 preadipocytes were stimulated withDMEM containing 10% FBS, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 5 μg/ml insulin and 1 μM dexamethasone for differentiation to adipocytes. 3T3-L1 cells were cultured with PBS or water extract of pear pomace. Water extract of pear pomace effectively inhibited lipid accumulations andexpressions of PPAR-γ and C/EBPα in 3T3-L1 cells. It also increased expression of p-AMPK and decreased the expression of SREBP-1c andFAS in 3T3-L1 cells. The induction of apoptosis was observed in 3T3-L1 cells treated with pear pomace. These results indicate that pear pomacewater extract inhibits adipogenesis and induces apoptosis of adipocytes and thus can be used as a potential therapeutic substance as part of preventionor treatment strategy for obesity.
Abstract
Obesity occurs when a person’s calorie intake exceeds the amount of energy burns, which may lead to pathologic growth of adipocytes andthe accumulation of fat in the tissues. In this study, the effect and mechanism of pear pomace extracts on 3T3-L1 adipocyte differentiation andapoptosis of mature adipocytes were investigated. The effects of pear pomace extract on cell viability and the anti-adipogenic and proapoptoticeffects were investigated via MTT assay, Oil red O staining, western blot analysis and apoptosis assay. 3T3-L1 preadipocytes were stimulated withDMEM containing 10% FBS, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 5 μg/ml insulin and 1 μM dexamethasone for differentiation to adipocytes. 3T3-L1 cells were cultured with PBS or water extract of pear pomace. Water extract of pear pomace effectively inhibited lipid accumulations andexpressions of PPAR-γ and C/EBPα in 3T3-L1 cells. It also increased expression of p-AMPK and decreased the expression of SREBP-1c andFAS in 3T3-L1 cells. The induction of apoptosis was observed in 3T3-L1 cells treated with pear pomace. These results indicate that pear pomacewater extract inhibits adipogenesis and induces apoptosis of adipocytes and thus can be used as a potential therapeutic substance as part of preventionor treatment strategy for obesity.
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