Anti-obese effects of mulberry (Morus alba L.) root bark through the inhibition of digestive enzymes and 3T3-L1 adipocyte differentiation
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Anti-obese effects of mulberry (Morus alba L.) root bark was investigated in vitro by measuring its inhibitory effect against 3T3-L1 preadipocyte differentiation and digestive enzymes such as α-amylase, α-glucosidase and pancreatic lipase. Ethanol extract of mulberry root bark (MRE) showed the potent inhibitory activities on α-amylase, α-glucosidase and pancreatic lipase with IC50 values of 7.86±0.36, 0.12±0.03 and 7.93±0.11 mg/mL, respectively. Furthermore, MRE significantly suppressed cellular lipid accumulation in 3T3-L1 cells in a dose-dependent manner. To elucidate the mechanism of MRE, we performed qRT-PCR and Western blotting for the expression of genes related with adipogenesis and lipogenesis. Treatment of MRE markedly suppressed the protein expression of PPARγ, C/EBPα and SREBP-1c, as well as FAS and ACC, which are the key transcription factors and metabolic enzymes in adipogenesis and lipogenesis. In addition, qRT-PCR analysis indicated that the anti-adipogenesis effect of MRE might be due to its inhibition at transcription levels. These results demonstrate that MRE can effectively suppress adipocyte differentiation and inhibit key enzymes related to obesity. Our findings suggest that mulberry root bark may have a potential benefit in preventing obesity.
Abstract
Anti-obese effects of mulberry (Morus alba L.) root bark was investigated in vitro by measuring its inhibitory effect against 3T3-L1 preadipocyte differentiation and digestive enzymes such as α-amylase, α-glucosidase and pancreatic lipase. Ethanol extract of mulberry root bark (MRE) showed the potent inhibitory activities on α-amylase, α-glucosidase and pancreatic lipase with IC50 values of 7.86±0.36, 0.12±0.03 and 7.93±0.11 mg/mL, respectively. Furthermore, MRE significantly suppressed cellular lipid accumulation in 3T3-L1 cells in a dose-dependent manner. To elucidate the mechanism of MRE, we performed qRT-PCR and Western blotting for the expression of genes related with adipogenesis and lipogenesis. Treatment of MRE markedly suppressed the protein expression of PPARγ, C/EBPα and SREBP-1c, as well as FAS and ACC, which are the key transcription factors and metabolic enzymes in adipogenesis and lipogenesis. In addition, qRT-PCR analysis indicated that the anti-adipogenesis effect of MRE might be due to its inhibition at transcription levels. These results demonstrate that MRE can effectively suppress adipocyte differentiation and inhibit key enzymes related to obesity. Our findings suggest that mulberry root bark may have a potential benefit in preventing obesity.
참고문헌 (36)
- Barr EL, "HOMA insulin sensitivity index and the risk of all-cause mortality and cardiovascular disease events in the general population : the Australian diabetes, obesity and lifestyle study", Diabetologia, 53, pp.79~88 (2010)
- Liberopoulos EU, "Diagnosis and management of the metabolic syndrome in obesity", Obes Rev, 6, pp.283~296 (2005)
- Mokdad AH, "Prevalence of obesity, diabetes, and obesityrelated health risk factors", JAMA, 289, pp.76~79 (2003)
- Nammi S, "Obesity : an overview on its current perspectives and treatment options", Nutr J, 3, pp.3~10 (2004)
- Pischon T, "Obesity and cancer", Pro Nutr Soc, 67, pp.128~145 (2008)
- Shamseddeen H, "Epidemiology and economic impact of obesity and type 2 diabetes", Surg Clin N Am, 91, pp.1163~1172 (2011)
- Evans RM, "PPARs and the complex journey to obesity", Nat Med, 10, pp.355~361 (2004)
- An Y, "Inhibitory effects of flavonoids from Abelmoschus manihot flowers on triglyceride accumulation in 3T3-L1 adipocytes", Fitoterapia, 82, pp.595~600 (2011)
- Spiegelman BM, "Regulation of adipocyte gene expression in differentiation and syndromes of obesity", diabetes, 1993, pp.268, 6823~6826
- Farmer SR, "Transcriptional control of adipocyte formation", Cell Metab, 4, pp.263~273 (2006)
- Gregoire FM, "Understanding adipocyte differentiation", Physiol Rev, 78, pp.783~809 (1998)
- Rosen ED, "Transcriptional regulation of adipogenesis", Genes Dev, 14, pp.1293~1307 (2000)
- Tabor DE, "Identification of conserved cis-elements and transcription factors required for sterol-regulated transcription of stearoyl-CoA desaturase 1 and 2", J Biol Chem, 274, pp.20603~20610 (1999)
- Chang LW, "Antioxidant and antityrosinase activity of mulberry(Morus alba L. )twigs and root bark", Food Chem Toxicol, 49, pp.785~790 (2011)
- Yang XL, "Hypolipidemic and antioxidant effects of mulberry(Morus alba L. )fruit in hyperlipidaemia rats", Food Chem Toxicol, 48, pp.2374~2379 (2010)
- Kim HJ, "Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4+CD25+Foxp3+ regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model", J Ethnopharmacol, 138, pp.40~46 (2011)
- Lee MS, "Antidepressant-like effects of Cortex Mori Radicis extract via bidirectional phosphorylation of glucocorticoid receptors in the hippocampus", Behav Brain Res, 236, pp.56~61 (2013)
- Chao WW, "The production of nitric oxide and prostaglanding E2 in peritoneal macrophages is inhibited by Andrographis paniculata, Angelica sinensis and Morus alba ethyl acetate fractions", J Ethnopharmacol, 122, pp.68~75 (2009)
- Du J, "Antiviral flavonoids from the root bark of Morus alba L", Phytochemistry, 62, pp.1235~1238 (2003)
- EI-Beshbishy HA, "Hypolipidemic and antioxidant effects of Morus alba L. (Egyptian mulberry)root bark fractions supplementation in cholesterol-fedrats", Life Sci, 78, pp.2724~2733 (2006)
- Zhang M, "In vivo hypoglycemic effects of phenolics from the root bark of Morus alba", Fitoterapia, 80, pp.475~477 (2009)
- Chi YS, "Effects of naturally occurring prenylated flavonoids on enzymes metabolizing arachidonic acid: cyclooxygenases and lipoxygenases", Biochem Pharmacol, 62, pp.1185~1191 (2001)
- Sohn HY, "Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants : Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera(L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai", Phytomedicine, 11, pp.666~672 (2004)
- Zheng ZP, "Tyrosinase inhibition constituents from the roots of Morus australis", Fitoterapia, 83, pp.1008~1013 (2012)
- Chen HD, "Morusalbanol A, a neuro-protective Dielse Alder adduct with an unprecedented architecture from Morus alba", Tetrahedron, 68, pp.6054~6058 (2012)
- Ali H, "Alpha- amylase inhibitory activity of some Malaysian plants used to treat diabetes : with particular reference to Phyllanthus amarus", J Ethnopharmacol, 107, pp.449~455 (2006)
- Kim YM, "Inhibitory effect of pine extract on alpha-glucosidase activity and postprandial hyperglycaemia", Nutrition, 21, pp.756~761 (2005)
- Kim JH, "Development of inhibitors against lipase and α-glucosidase from derivatives of monascus pigment", FEMS Microbiol Lett, 276, pp.93~98 (2007)
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxicity assays", J Immunol Methods, 65, pp.55~63 (1983)
- Bhandari MR, "α-Glucosidase and α-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed(Bergenia ciliata, Haw. )", Food Chem, 106, pp.247~252 (2008)
- Liu S, "Inhibition of pancreatic lipase, α-glucosidase, α-amylase, and hypolipidemic effects of the total flavonoids from Nelumbo nucifera leaves", J Ethnopharmacol, 149, pp.263~269 (2013)
- Lowe ME, "Pancreatic triglyceride lipase and colipase : insights into dietary fat digestion", Gastroenterology, 107, pp.1524~1536 (1994)
- de la Garza AL, "Natural inhibitors of pancreatic lipase as new players in obesity treatment", Planta Med, 77, pp.773~785 (2011)
- Cho, Eun Jae, "Chitosan Oligosaccharides Inhibit Adipogenesis in 3T3-L1 Adipocytes", Journal of Microbiology and Biotechnology, 18(1), pp.80~87 (2008), 한국미생물·생명공학회
- Hasani-Ranjbar S, "A systematic review of the efficacy and safety of herbal medicines used in the treatment of obesity", World J Gastroenterol, 15, pp.3073~3085 (2009)
- Kwon TH, "6, 6′-Bieckol inhibits adipocyte differentiation through downregulation of adipogenesis and lipogenesis in 3T3-L1 cells", J Sci Food Agric (2014)
