튤립(Liriodendron tulipifera) 나무가지 메탄올 추출물의 항산화와 항암활성 효과
The Antioxidant and Anticancer Effects of MeOH Extract of Liriodendron tulipifera
1
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
본 연구는 새로운 기능성 소재를 탐색하기 위하여 튤립나무 가지의 메탄올 추출물을 조제하여 생리활성 물질 함량, DPPH, 환원능력, Fe2+ chelating효과와 지질과산화 억제 활성 그리고 세포독성을 측정하였다. 총 페놀성 화합물과 플라보노이드 함량은 75.34 mg gallic acid/g과 20.15 mg quercetin/g이고; DPPH 라디칼 소거 활성에서 EC50은 289.68 ± 2.04 μg/mL; 환원력 측정에서 100 μg/mL 농도에서 흡광도는 0.388이었으며, Fe2+ chelating 효과에서는 200 μg/mL 농도에서 36.33%로 나타내었고, 지질과산화 억제 효능에서는 200 μg/mL의 농도에서 비교적 높은38.56%의 억제율을 나타내었으며, 암세포 증식 억제 효과에서는 HT-29와 Hela cell line에서 튤립나무가지 메탄올 추출물이 200 μg/mL의 농도에서 56.94%와 35.73%의 세포생장 억제율을 나타내었다. 따라서 본 연구 결과들을 종합해 볼 때 항산화능력은 총 페놀성분과 밀접한 관계가 있는 것으로 사료되며, 튤립나무 가지에 대한 기타 생리활성 연구가 더 필요할 것으로 사료된다.
Abstract
In order to screen the functional constituents from nature resource, we studied the bioactivities of methanol extract of the Liriodendron tulipifera branch(MLT). The total phenolic and flavonoid contents, DPPH radical scavenging capacity, reducing power, Fe2+ chelating ability, inhibition of lipid peroxidation and cell toxicity of MLT were investigated in this study. We found that the total phenolic and flavonoid content of MLT is 75.34 mg gallic acid/g and 20.15 mg quercetin/g respectively. MLT exhibited the antioxidant activity on DPPH radical with a EC50 value of 289.68 μg/mL , the absorbance is 0.388 at 100 μg/mL in reducing power assay, MLT prevented 38.56% lipid peroxidation at 200 μg/mL. Furthermore, MLT exhibits the potent anti-proliferative activity which inhibited 56.94%, 35.73% growth of HT-29 and Hela cell at 200 μg/mL respectively. It showed that the antioxidant activities of MLT were correlated with its total phenolic and flavonoid contents. However further study need to be exploring in the future.
참고문헌 (35)
- Banerjee, A., "In vitro study of antioxidant activity of Syzygium cumini fruit", Food Chem, 90, pp.727~733 (2005)
- Birt, B.F., "Dietary agents in cancer prevention: flavonoids and isoflavoids", Pharmacol. Ther, 90, pp.157~177 (2001)
- Blois, M.S., "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Buchanan, M.A., "Liriodenine, a nitrogen-containing pigment of yellow poplar heartwood (Liriodendron tulipifera L.)", J. Org. Chem, 25, pp.1389~1391 (1960)
- Chang, C.C., "Estimation of total flavonoid content in propolis by two complementary colorimetric methods", J. of Food and Drug Analysis, 10, pp.178~182 (2002)
- Chen, C.L., "Aporphine alkaloids and lignans in heartwood of Liriodendron tulipifera", Phytochemistry, 15, pp.547~550 (1976)
- Chen, C.L., "Aporphine alkaloids and lignans formed in response to injury of sapwood in Liriodendron tulipifera", Phytochemistry, 15, pp.1161~1167 (1976)
- Chen, C.L., "Lignans and aporphine alkaloids in bark of Liriodendron tulipifera", Phytochemistry, 17, pp.779~782 (1978)
- 최장숙, "밤꽃(Castanea Crenata Flos.) 메탄올 추출물의 항산화 효과", 한국식품과학회지, 35(6), pp.1216~1220 (2003), 한국식품과학회
- 최선영, "식용 및 약용자원의 에스트로젠 활성과 항산화능 평가", 한국식품과학회지, 37(4), pp.549~556 (2005), 한국식품과학회
- Cohen, J., "Notesthe alkaloids of Liriodendron tulipifera L. The structure and synthesis of the unnamed yellow alkaloid and the isolation of D-glaucine", J. Org. Chem, 26, pp.4143~4144 (1961)
- Doskotch, R.W., "Antitumor agents. II. Tulipinolide, a new ger-macranolide sesquiterpene, and constunolide. Two cytotoxic substances from Liriodendron tulipifera L", J. Pharm. Sci, 58, pp.877~880 (1969)
- Doskotch, R.W., "The structure of tulipinolide and epitulipino- lide. Cytotoxic sesquiterpenes from Liriodendron tulipifera L", J. Org. Chem, 35, pp.1928~1936 (1970)
- Doskotch, R.W., "Peroxyferolide: a cytotoxic gerrnacranolide hydroperoxide from Liriodendron tulipifera", J. Chem. Soc. Chem. Comm, pp.402~403 (1976)
- Doskotch, R.W., "Isolation and characterization of peroxyferolide, a hydroperoxy sesquiterpene lactone from Liriodendron tulipifera", J. Org. Chem, 42, pp.3614~3618 (1977)
- Doskotch, R.W., "New sesquiterpene lactones from Liriodendron tulipifera", Phytochemistry, 14, pp.769~773 (1975)
- 엄석현, "원적외선 조사에 따른 가래나무 잎의 항산화 활성 변화", 한국약용작물학회지, 15(4), pp.266~270 (2007), 한국약용작물학회
- 고은정, "황기(Astragalus membranaceus)의 박피 유무와 재배 년 수에 따른 항산화 활성 연구", 한국약용작물학회지, 17(4), pp.233~237 (2009), 한국약용작물학회
- Gordon, M.F., "The mechanism of antioxidant action in vitro. In Food antioxidants", pp.1~8 (1990), Elsevier Applied Science, London, UK
- Graziose, R., "Antiplasmodial activity of aporphine alkaloids and sesquiterpene lactones from Liriodendron tulipifera L", J. of Ethnopharmacology (2010)
- Green, L.M., "Rapid colometric assay for cell viability: Application to the quantitation of cytotoxic and growth inhibitory lymphokines", J. of Immunological Methods, 70, pp.257~268 (1984)
- Hasegawa, G.R., "Quinine substitutes in the confederate army", Military Medicine, 172, pp.650~655 (2007)
- ng Y.H., "Studies on the physiological functionality of ine needle and mugwort extracts", Kor. J. Food Sci. Technol, 27, pp.978~984 (1995)
- 김혜숙, "참외 비식용부위(꼭지, 줄기․잎, 씨) 에탄올추출물의 항산화 활성", 한국자원식물학회지, 23(5), pp.451~457 (2010), 한국자원식물학회
- Kim, T.W., "The woody plants of Korea in color", pp.101~ (1995), Kyo- Hak publishing Co. Ltd. Seoul
- Lee, J.H., "Analysis of phenolic substances content in Korean plants foods", Kor. J. Food Sci. Technol, 26, pp.310~316 (1994)
- 이연리, "발아 거대배아미의 생리활성 효과 검정", Journal of the Korean Society for Applied Biological Chemistry, 47(2), pp.216~221 (2004), 한국응용생명화학회
- Lin, J.Y., "Determination of total phenolic and flavonoid contents in selected freits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation", Food Chem, 101, pp.140~147 (2007)
- Merkle, S.A., "Yellow-poplar (Liriodendron spp.) In: Biotechnology in agriculture and forestry, trees III, Vol.16", pp.94~110 (1991), Springer-verlag, Berlin
- Muhammad, I., "Phenylpropanoids, sesquiterpenes, and alka- loids from the seeds of Liriodendron tulipifera", J. Nat. Prod, 52, pp.1177~1179 (1989)
- Rafinesque, C.S., "Medical Flora, or, Manual of the Medical Botany of the United States of North America" (1828), Alexander, Philadelphia
- 임태진, "개머루덩굴 추출물의 항산화 효과", 한국자원식물학회지, 23(5), pp.445~450 (2010), 한국자원식물학회
- Spencer, C.F., "Survey of plants for antimalarial activity", Lloydia, 10, pp.145~174 (1947)
- Taylor, W.I., "The structure and synthesis of liriodenine, a new type of isoquino- line alkaloid", Tetrahedron, 14, pp.42~45 (1961)
- Yen, G.C., "Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid", Food Chem, 79, pp.307~313 (2002)