멸종위기 식물 날개하늘나리의 항산화 및 항암활성
Characterization of Antioxidant Activity and Anticancer Effect of an Endangered Plant, Lilium dauricum Ker-Gawl
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Background: Lilium dauricum Ker-Gawl is an endangered plant, and it is highly valued as a medicinal plant owing to its excellent functionality. Methods and Results: L. dauricum leaf extract was shown to have high antioxidant activity and an inhibition concentration of 50% (IC50)value of 71.8 ± 0.13 ㎍/㎖ was shown for 2,2-diphenyl- 1-picrylhydrazyl radical scavenging activity. Its total phenol and flavonoid contents were 74.76 ± 0.24 ㎎·gallic acid equivalent (GAE)/g and 31.44 ± 0.27 ㎎·quercetin equivalent (QE)/g, respec- tively. Nitric oxide (NO) production was measured after oxidative stress was induced in lipopoly- saccharide-stimulated RAW 264.7 cells, and 200 ㎍/㎖ of leaf extract effectively inhibited NO production at a rate of 17.84 ± 0.18%. Gastric cancer cells (AGS) and lung cancer cells (A549) were treated with 200 ㎍/㎖ of bulb extract, the inhibition rate for gastric cancer cell growth was 48.12 ± 2.60% whereas the inhibition rate for lung cancer cell growth was 55.83 ± 4.08%. Conclusions: These results provide basic research data that can be used to identify the active con- stituents present in L. dauricum leaf and bulb extracts for the development of anticancer drugs, antioxidants, and functional products.
Abstract
Background: Lilium dauricum Ker-Gawl is an endangered plant, and it is highly valued as a medicinal plant owing to its excellent functionality. Methods and Results: L. dauricum leaf extract was shown to have high antioxidant activity and an inhibition concentration of 50% (IC50)value of 71.8 ± 0.13 ㎍/㎖ was shown for 2,2-diphenyl- 1-picrylhydrazyl radical scavenging activity. Its total phenol and flavonoid contents were 74.76 ± 0.24 ㎎·gallic acid equivalent (GAE)/g and 31.44 ± 0.27 ㎎·quercetin equivalent (QE)/g, respec- tively. Nitric oxide (NO) production was measured after oxidative stress was induced in lipopoly- saccharide-stimulated RAW 264.7 cells, and 200 ㎍/㎖ of leaf extract effectively inhibited NO production at a rate of 17.84 ± 0.18%. Gastric cancer cells (AGS) and lung cancer cells (A549) were treated with 200 ㎍/㎖ of bulb extract, the inhibition rate for gastric cancer cell growth was 48.12 ± 2.60% whereas the inhibition rate for lung cancer cell growth was 55.83 ± 4.08%. Conclusions: These results provide basic research data that can be used to identify the active con- stituents present in L. dauricum leaf and bulb extracts for the development of anticancer drugs, antioxidants, and functional products.
참고문헌 (30)
- Bengmark S, "Plant-derived health : The effects of turmeric and curcuminoids", Nutrición Hospitalaria, 24, pp.273~281 (2009)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Chen F, "Biosynthesis and emission of terpenoid volatiles from Arabidopsis flowers", The Plant Cell, 15, pp.481~494 (2003)
- 최문희, "국산 블루베리 착즙액의 안토시아닌 분석 및 RAW267.4 세포주에서의 항염효과", KSBB Journal, 30(4), pp.191~196 (2015), 한국생물공학회
- Chu YH, "Flavonoid content of several vegetables and their antioxidant activity", Journal of the Science of Food and Agriculture, 80, pp.561~566 (2000)
- Geronikaki AA, "Antioxidants and inflammatory disease : Synthetic and natural antioxidants with anti-inflammatory activity", Combinatorial Chemistry and High Throughput Screening, 9, pp.425~442 (2006)
- 한혜민, "마름 추출물의 항산화활성 및 암세포증식 억제 활성", 한국약용작물학회지, 24(1), pp.14~20 (2016), 한국약용작물학회[DOI]
- Karna P, "Benefits of whole ginger extract in prostate cancer", British Journal of Nutrition, 107, pp.473~484 (2012)
- Kim HK, "In vitro mass propagation and biological activities of Lilium dauricum Ker Gawl", Ph. D, pp.2020
- Seong-Cheol Bang, "Germination-Inhibitory Effect of Pulsatilla koreana N. Leaves;Protoanemonin as Active Principle", Journal of Applied Biological Chemistry, 48(2), pp.89~92 (2005), 한국응용생명화학회
- Kondo T, "Roles of oxidative stress and redox regulation in atherosclerosis", Journal of Atherosclerosis and Thrombosis, 6, pp.532~538 (2009)
- 구강모, "고추 기관별 항산화 활성과 quinone reductase 유도활성", Journal of Applied Biological Chemistry, 53(1), pp.31~36 (2010), 한국응용생명화학회
- Kumar S, "Antioxidant and free radical scavenging potential of Citrullus colocynthis(L. )Schrad. methanolic fruit extract", Acta Pharmaceutica, 58, pp.215~220 (2008)
- 이환, "비파 부위별 에탄올 추출물의 항산화 활성 및 암세포 증식 억제효과", 한국지역사회생활과학회지, 27(2), pp.211~220 (2016), 한국지역사회생활과학회
- 이희경, "엉겅퀴 추출물의 항상화성, 항돌연변이원성 및 항암활성 효과", 한국약용작물학회지, 11(1), pp.58~61 (2003), 한국약용작물학회
- Lee JL, "Antiwrinkle effect of Morinda citrifolia(noni)extracts", Journal of the Society of Cosmetic Scientists of Korea, 32, pp.227~231 (2006)
- 이광재, "황기와 몽고황기 추출물의 항산화 활성 및 Nitric Oxide 생성능", 한국식품영양과학회지, 40(12), pp.1793~1796 (2011), 한국식품영양과학회
- Liu RH, "Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals", American Journal of Clinical Nutrition, 78, pp.517S~520S (2003)
- 목지예, "엉겅퀴(Cirsium japonicum var. ussuriense) 부위별 추출물의 항산화 및 항염증 효과", 대한본초학회지, 26(4), pp.39~47 (2011), 대한본초학회
- Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", Journal of Ethnopharmacology, 71, pp.109~114 (2000)
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxicity assays", Journal of Immunological Methods, 65, pp.55~63 (1983)
- 박복희, "구기자 용매추출물과 구기자 매작과의 항산화 효과", 한국식품영양과학회지, 34(9), pp.1314~1319 (2005), 한국식품영양과학회
- 박미진, "하늘타리(Trichosanthes kirilowii Maxim.) 부위별 추출물의 항산화 및 항염증 활성", 생약학회지, 47(4), pp.327~332 (2016), 한국생약학회
- 박성진, "당귀 잎의 항산화 활성", Food Science and Preservation, 18(6), pp.817~823 (2011), 한국식품저장유통학회
- 박영숙, "한약재 추출물의 폴리페놀 화합물과 항산화 활성", 동아시아식생활학회지, 12(1), pp.23~31 (2002), 동아시아식생활학회
- Reichelt M, "Benzoic acid glucosinolate esters and other glucosinolates from Arabidopsis thaliana", Phytochemistry, 59, pp.663~671 (2002)
- 서훈석, "짚신나물, 삼백초의 항산화와 항암활성 효과", 한국약용작물학회지, 16(3), pp.139~143 (2008), 한국약용작물학회
- Singleton VL, "Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents", American Journal of Enology and Viticulture, 16, pp.144~158 (1965)
- Vijaya K, "Antibacterial effect of the aflavin, polyphenon 60(Camellia sinensis)and Euphorbia hirta on Shigella spp. － a cell culture study", Journal of Ethnopharmacology, 49, pp.115~121 (1995)
- 유미희, "생대추(풋대추) 과육 및 씨 추출물의 항산화 효과 및 항산화 성분", 한국식품과학회지, 38(1), pp.128~134 (2006), 한국식품과학회