Phylogenetic Relationships in Korean Elaeagnus L. Based on nrDNA ITS Sequences
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초록
Molecular phylogenetic analyses of Korean Elaeagnus L. were conducted using seven species, one variety, one forma and four outgroups to evaluate their relationships and phylogeny. The sequences of internal transcribed spacer regions in nuclear ribosomal DNA were employed to construct phylogenetic relationships using maximum parsimony (MP) and Bayesian analysis. Molecular phylogenetic analysis revealed that Korean Elaeagnus was a polyphyly. E. umbellata var. coreana formed a subclade with E. umbellata. Additionally, the genetic difference between E. submacrophylla and E. macrophylla was very low. Moreover, E. submacrophylla formed a branch from E. macrophylla, indicating that E. submacrophylla can be regarded as a variety. However, several populations of this species were not clustered as a single clade; therefore, further study should be conducted using other molecular markers. Although E. glabra f. oxyphylla was distinct in morphological characters of leaf shape with E. glabra. But E. glabra f. oxyphylla was formed one clade by molecular phylogenetic with E. glabra. Additionally, this study clearly demonstrated that E. pungens occurs in Korea, although it was previously reported near South Korea in Japan and China. According to the results of ITS regions analyses, it showed a resolution and to verify the relationship between interspecies of Korean Elaeagnus.
Abstract
Molecular phylogenetic analyses of Korean Elaeagnus L. were conducted using seven species, one variety, one forma and four outgroups to evaluate their relationships and phylogeny. The sequences of internal transcribed spacer regions in nuclear ribosomal DNA were employed to construct phylogenetic relationships using maximum parsimony (MP) and Bayesian analysis. Molecular phylogenetic analysis revealed that Korean Elaeagnus was a polyphyly. E. umbellata var. coreana formed a subclade with E. umbellata. Additionally, the genetic difference between E. submacrophylla and E. macrophylla was very low. Moreover, E. submacrophylla formed a branch from E. macrophylla, indicating that E. submacrophylla can be regarded as a variety. However, several populations of this species were not clustered as a single clade; therefore, further study should be conducted using other molecular markers. Although E. glabra f. oxyphylla was distinct in morphological characters of leaf shape with E. glabra. But E. glabra f. oxyphylla was formed one clade by molecular phylogenetic with E. glabra. Additionally, this study clearly demonstrated that E. pungens occurs in Korea, although it was previously reported near South Korea in Japan and China. According to the results of ITS regions analyses, it showed a resolution and to verify the relationship between interspecies of Korean Elaeagnus.
참고문헌 (42)
- Ainouche, M.L., "On the origins of the tetraploid Bromus species (section Bromus, Poaceae): insights from internal transcribed spacer sequences of nrDNA", Genome, 40, pp.730~743 (1997)
- Baldwin, B.G., "Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositae", Mol. Phylogent Evol, 1, pp.3~16 (1992)
- Britton, N.L., "An Illustrated Flora of The Northern United States", pp.575~ (1913), Charles Scribner’s Sons
- Chang, C.Y., "Elaeagnus L.: Flora Reipublicae Popularis Sinicae", pp.1~60 (1983), Science Press
- Cronquist, A., "An Intergrated System of Classification of Flowering Plants", pp.606~607 (1981), Colurmbia Univ. Press
- "Geneious v5.5" (2011)[URL]
- Felsenstein, J., "Confidence limits on phylogenies: an approach using the bootstrap", Evolution, 39(4), pp.783~791 (1985)
- Fu, Y.C, "Advances on chemical constituents and pharmacological activities from plants of Elaeagnus L", Qilu Pharmaceutical Affairs, 26, pp.232~233 (2007)
- Hemsley, W.B., "An enumeration of all the plants known form China (Index Florae Sinensis)", Jour. Linn. Sco. Bot, 26, pp.1~592 (1894)
- Heywood, V.H., "Flowering plant families of the world", pp.135~136 (2007), Kew Publishing
- Kaplan, Z, "Molecular evidence for a natural primary triple hybrid on plants revealed from direct sequencing", Ann. Bot, 99, pp.1213~1222 (2007)
- Ki, K.R., "Contribution of trichome morphology to the taxonomy of Korean Elaeagnus species", 석사, pp.2004
- 김성애, "뜰보리수 열매의 용매분획별 항산화 및 암세포 증식 억제효과", 한국식품영양학회지, 20(2), pp.134~142 (2007), 한국식품영양학회
- Kim, K.J., "Comparisons of phylogenetic hypotheses among the different data sets in dwarf dadelion (krigia, Asteraceae): Additional information from internal transcribed spacer sequences of nuclear ribosomal DNA", Plant Syst. Evol, 190, pp.157~185 (1994)
- Koh, J.E., "A taxonomic study of the Korean Elaeagnus L.(Elaeagnaceae)", 석사, pp.2005
- 박종희, "민간약 보리수나무의 생약학적 연구", 생약학회지, 42(1), pp.1~5 (2011), 한국생약학회
- Lee, D.B., "Taxonomic Study on the Genus Elaeagnus in Korea", 석사, pp.2006
- Jin Hwan Lee, "Determination of Phytochemical Contents and Biological Activities from the Fruits of Elaeagnus multiflora", Preventive Nutrition and Food Science, 16(1), pp.29~36 (2011), 한국식품영양과학회
- Lee, S.T., "The Genera of Vascular Plants of Korea" (2007), Flora of Korea Editorial Committee
- Lee, T.B., "Illustrated Flora of Korea", pp.561~562 (1980), Hyangmunsa, Publishing Co
- Lee, W.T., "Lineamenta Florae Koreae", pp.713~716 (1996), Academy Publishing Co
- Linnaeus, C., "Species Plantarum", 121~
- Loockerman, D.J., "Sampling the Green World", pp.205~220 (1996), Columbia Univ. Press
- Mcneill, J., "International Code of Botanical Nemenclature for Algae, Fungi and Plant (Melbourne Code)", A. R. G. Gantner Verlag, ~ (2012)
- Mori, T., "An Enumertion of Plants Hitherto Known from Corea", pp.259~260 (1921), Gov. Chos
- Nakai, T., "Elaeagnus. Flora Koreana", J. Coll. Sci. Imp, 31, pp.178~179 (1911)
- Nakai, T., "Elaeagnus Japoniae", Coreae et Formosae. Bot. Mag, 30, pp.72~76 (1916)
- Nakai, T., "Elaeagnus. In A Synoptical Sketch of Korean Flora", pp.31~75 (1952), Bull. Natl. Sci. Mus
- Ohba, H., "Flora of Japan. Vol. IIc", pp.152~158 (2003), Kodansha
- Ohwi, J., "Elaeagnus. In Flora off Japan", pp.646~648 (1984), Smithsonian Institution
- Palibin, J., "Conspectus florae. Pars. secunda. trudy Imo. S.-Peterburgsk", Bot. Sada, 18, pp.186~187 (1900)
- Ronquist, F., "MrBayes3: Bayesian phylogenetic inference under mixed models", Bioinformatics, 19, pp.1572~1574 (2003)
- Schlechtendal DFL., "Elaeagnaceae", Prodromus Systematis Naturalis Regni Vegetabilis, 14, pp.606~616 (1857)
- Schlechtendal DFL, "Elaeagnacearum in Candollei prodromo (Vol. XIV) expositarum adumbrates", Linnaea, 30, pp.304~386 (1860)
- Servettaz, C., "Note preliminaire sur la Systematique des Elaeagnacees", pp.381~394 (1908), serie Tome VIII
- Schlechtendal DFL., "Monographie des Elaeagnacees in Beihefte Botanischen Centralbatt", 25, pp.1~128 (1909)
- Sun, M., "A revision of Elaeagnus L. (Elaeagnaceae) in mainland China", J. Syst. Evol, 48, pp.356~390 (2010)
- Sun, X., "Molecular phylogenetics of Hippophae L.(Elaeagnaceae) based on the internal transcribed spacer (ITS) sequences of nrDNA", Plant Syst. Evol, 235, pp.121~134 (2002)
- Swofford, D.L., "PAUP*: Phylogenetic Analysis Using Parsimony (*andothermethods)", Version.4. Sinauer Associates (2003)
- Wang, Y., "Nutritional composition of wild Elaeagnus angustifolia fruits", Journal of Gansu Agricultural Univ. China, 41, pp.6~28 (2006)
- White, T.J., "PCR Protocols: A guide to methods and applications", pp.315~322 (1990), Academic Press
- 유광필, "ITS 염기서열에 의한 한국산 담배풀속(Carpesium L.)의 계통분류학적 연구", 한국자원식물학회지, 25(1), pp.96~104 (2012), 한국자원식물학회