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Phylogenetic Analysis on the Seven Species of Theaceae Growing in Korea Based on RAPD and ITS Sequences of Nuclear Ribosomal

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초록

The genetic relationships among five genera, seven species of Theaceae were examined through a random amplified polymorphic DNA (RAPD) analysis and internal transcribed spacer (ITS). In RAPD analysis, five of 15 arbitrary primers showed polymorphic bands, which were able to classify different genera and species of Theaceae. The genetic variations of Theaceae were from 0.031 to 0.484. In ITS analysis, the ITS sequences were analyzed using BLAST and showed high identities with sequences of Theaceae, seven species published in NCBI GenBank database, which ranged from 98 to 100%. Sequence alignment of seven species showed 34.9% identities for ITS 1 region and 43.7% for ITS 2 region. Pairwise sequence divergences among seven species ranged from 0 to 0.330%. In phylogenetic tree, they were divided into three groups. In conclusion, the molecular data generated in the present investigation will help to understand the genetic relationships of Theaceae and also might be useful for further studies in intra-species, inter-species, and molecular evolution researches.

Abstract

The genetic relationships among five genera, seven species of Theaceae were examined through a random amplified polymorphic DNA (RAPD) analysis and internal transcribed spacer (ITS). In RAPD analysis, five of 15 arbitrary primers showed polymorphic bands, which were able to classify different genera and species of Theaceae. The genetic variations of Theaceae were from 0.031 to 0.484. In ITS analysis, the ITS sequences were analyzed using BLAST and showed high identities with sequences of Theaceae, seven species published in NCBI GenBank database, which ranged from 98 to 100%. Sequence alignment of seven species showed 34.9% identities for ITS 1 region and 43.7% for ITS 2 region. Pairwise sequence divergences among seven species ranged from 0 to 0.330%. In phylogenetic tree, they were divided into three groups. In conclusion, the molecular data generated in the present investigation will help to understand the genetic relationships of Theaceae and also might be useful for further studies in intra-species, inter-species, and molecular evolution researches.

참고문헌 (33)

  1. Appels, R., "Relative rates of divergence of species and gene sequences within the rDNA region of species in the Triticeae: implications for the maintenance of homogeneity of a repeated gene family", Theor. Appl. Genet, 63, pp.361~365 (1982)
  2. Baldwin, B. G., "The ITS region of nuclear ribosomal DNA: A valuable source of evidence of angiosperm phylogeny", Ann. Missouri. Bot. Gard, 82, pp.247~277 (1995)
  3. Baldwin, B. G., "Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: an example from the Compositae", Mol. Phyl. Evol, 1, pp.3~16 (1992)
  4. Barker, R. F., "Structure and evolution of the intergenic region in a ribosomal DNA repeat unit of wheat", J. Mol. Biol, 201, pp.1~17 (1988)
  5. Chung, T. H., "Korea illustrated plant book. Woody plants" (1958), Shinjisa press
  6. Cronquist, A., "An integrated system of classification of flowering plants" (1981), Columbia University Press
  7. Hamby, R. K., "Ribosomal RNA as a phylogenetic tool in plant systematics. - In molecular systematics of plants", pp.50~91 (1992), Chapman and Hall
  8. Yong-HwanJung, "Phylogenetic Analysis of Korean Prunus (Rosaceae) Based on ITS Sequences of Nuclear Ribosomal DNA", Genes & Genomics, 24(3), pp.2~258 (2002), 한국유전학회
  9. 김동갑, "RAPD분석에 의한 미선나무속의 분류학적 연구", 한국자원식물학회지, 15(1), pp.18~24 (2002), 한국자원식물학회
  10. 김주환, "RAPD markers에 의한 한국산 때죽나무과(Styracaceae) 식물의 유연관계", 식물분류학회지, 37(2), pp.143~153 (2007), 한국식물분류학회
  11. Kim, K. J., "Comparisons of phylogenetic hypotheses among different data sets in dwarf dandelions (Krigia, Asteraceae): additional information from internal transcribed spacer sequences of nuclear ribosomal DNA", Pl. Syst. Evol, 190, pp.157~185 (1994)
  12. 김남희, "PCR-RAPD와 ITS 서열 분석에 의한 두릅나무과(Araliaceae) 의 유연관계 분석", 한국자원식물학회지, 17(2), pp.82~93 (2004), 한국자원식물학회
  13. Kim, S. S., "A systematic study of the Theaceae 6 species in Korea", Jour. Korean For. Soc, 82(4), pp.431~440 (1993)
  14. Kimura, M., "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences", J. of Mol. Evol, 16, pp.111~120 (1980)
  15. 권혜진, "노각나무(Stewartia koreana, 차나무과)의 분류학적 재검토", 식물분류학회지, 38(3), pp.233~250 (2008), 한국식물분류학회
  16. Lee, S. T., "A playnotaxonomic study on the Korea Theaceae", Korean J. Bot, 30(3), pp.215~223 (1987)
  17. Lee, T. B., "Illustrated flora of Korea" (1980), Hyangmunsa press
  18. Lee, T. B., "Coloured flora of Korea" (2006), Hyangmunsa press
  19. Lee, Y. N., "Flora of Korea" (1996), Gyohaksa press
  20. Nakai., "The Korea plants" (1909), Seongmidang press
  21. Nakai., "Synoptical sketch of Korean flora", Bull. Nat. Sci. Museum (1952)
  22. Nei, M., "Mathemarical model for studying genetic variation in terms of restriction endonuclease", Proc. Natl. Acad. Soc, 76, pp.5269~5273 (1979)
  23. 김주환, "RAPD에 의한 한국산 노린재나무과 식물의 유연관계 분석", 식물분류학회지, 37(3), pp.225~237 (2007), 한국식물분류학회
  24. 신세균, "연의 ITS 염기 서열 분석에 의한 유연 관계 분석", Horticulture, Environment, and Biotechnology, 44(4), pp.451~457 (2003), 한국원예학회
  25. Soh, W. Y., "Systematic studies on some Korean woody plants –A comparative wood anatomy of Theaceae-", Korean J. Bot, 29(4), pp.317~327 (1986)
  26. Sudheer Pamidimarri, D. V. N., "Genetic divergence and phylogenetic analysis of genus Jatropha based on nuclear ribosomal DNA ITS sequence", Mol. Biol. Rep, 36, pp.1929~1935 (2009)
  27. Yan-Lin Sun, "Genetic diversity of Kalopanax pictus populations in Korea based on the nrDNA ITS sequence", Journal of Plant Biotechnology, 39(1), pp.75~80 (2012), 한국식물생명공학회
  28. Tochika-Komatsu, Y., "A ramdom amplified polymorphic DNA (RAPD) primer to assist the identification of a selected strain, Aizu K-111 of Panax ginseng and the sequence amplified", Bio. Pharm. Bull, 24, pp.1210~1213 (2001)
  29. Torres, R. A., "GC balance in the internal transcribed spacers ITS1 and ITS2 nuclear ribosomal DNA", J. Mol. Ecol, 30, pp.170~181 (1990)
  30. Ueki, M., "The nucleotide sequence of the rRNA intergenic spacer region in a wild species of the genus Vicia, V. angustifolia", P1. Mol. Biol, 18, pp.175~178 (1992)
  31. White, T. J., "Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols: A guide to methods and applications", pp.315~322 (1990), Academic press
  32. Williams, J. G., "DNA polymorphisms amplified by arbitrary primers are useful as genetic markers", Nucl. Acids Res, 18, pp.6531~6535 (1990)
  33. Yokota, Y., "Nucleotide sequences of the 5´8S rRNA gene and internal transcribed spacer regions in carrot and broad bean ribosomal DNA", Journal of Molecular Evolution, 29, pp.294~301 (1989)

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