Analysis of Phylogenetic Relationship of 30 Cultivars of Korean Mulberry (Rosales: Moraceae) in Korea
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초록
This study was carried out to understand phylogenetic relationships of the 30 mulberry cultivars converved in Korea based on the ITS rDNA region, and they were compared to 40 reference sequences from GenBank. The size and the G+C content of the ITS rDNA gene regions from the 30 Korean mulberry cultivars and 40 reference sequences varied from 612– 630 bp and 58.19–61.62%, respectively. Based on the results of the comparative phylogenetic analysis of the ITS rDNA regions of the 30 Korean mulberry cultivars and 40 reference sequences, they were divided into three groups (Group 1, 2, and 3) and two subgroups (Group 1A and 1B within Group 1). The sequence lengths of the Korean mulberry cultivar numbers 1–26 and 27–30 were 615 bp and 616 bp, respectively. At 205 bp location of ITS1 rDNA region, the cultivar numbers 1–26 contain the nucleotide thymine but the cultivar numbers 27–30 contain the nucleotide adenine. In addition, the insertion of the nucleotide adenine at 206 bp location was found only in the four Korean mulberry cultivars (numbers 27–30). Based on these sequence information and phylogenetic result, the 30 Korean mulberry cultivars were identified as M. alba and M. australis. This study will contribute to the construction of genetic database constructions and accurate variety identifications for unidentified mulberry varieties in Korea.
Abstract
This study was carried out to understand phylogenetic relationships of the 30 mulberry cultivars converved in Korea based on the ITS rDNA region, and they were compared to 40 reference sequences from GenBank. The size and the G+C content of the ITS rDNA gene regions from the 30 Korean mulberry cultivars and 40 reference sequences varied from 612– 630 bp and 58.19–61.62%, respectively. Based on the results of the comparative phylogenetic analysis of the ITS rDNA regions of the 30 Korean mulberry cultivars and 40 reference sequences, they were divided into three groups (Group 1, 2, and 3) and two subgroups (Group 1A and 1B within Group 1). The sequence lengths of the Korean mulberry cultivar numbers 1–26 and 27–30 were 615 bp and 616 bp, respectively. At 205 bp location of ITS1 rDNA region, the cultivar numbers 1–26 contain the nucleotide thymine but the cultivar numbers 27–30 contain the nucleotide adenine. In addition, the insertion of the nucleotide adenine at 206 bp location was found only in the four Korean mulberry cultivars (numbers 27–30). Based on these sequence information and phylogenetic result, the 30 Korean mulberry cultivars were identified as M. alba and M. australis. This study will contribute to the construction of genetic database constructions and accurate variety identifications for unidentified mulberry varieties in Korea.
참고문헌 (31)
- Àlvarez I, "Ribosomal ITS sequences and plant phylogenetic inference", Mol Phylogenet Evol, 29, pp.417~434 (2003)
- Awasthi AK, "Genetic diversity and relationships in mulberry (genus Morus) as revealed by RAPD and ISSR marker assays", BMC Genet, 5(1) (2004)
- Brandis D, "The forest flora of northwest and central India. In Indian trees", pp.407~410 (1874), William H. Allen & Co.
- Bureau E, "Prodromus systematis naturalis regni vegetabilis", pp.211~288 (1873), Tuetteland Wurtz
- Cao H, "Methodological studies on genomic DNA extraction and purification from plant drug materials", J Chin Pharm Sci, 7, pp.130~137 (1998)
- Chen RF, "Analysis on the internal transcribed spacers (ITS) sequences and phylogenetics of mulberry (Morus)", Sci Agri Sinica, 43, pp.1771~1781 (2010)
- Felsenstein J, "Confidence limits on phylogenies: An approach using the bootstrap", Evolution, 39, pp.783~791 (1985)
- He N, "Draft genome sequence of the mulberry tree Morus notabilis", Nat Commun, 4, pp.1~9 (2013)
- Hotta T, "Fundamentals of Morus plants classification", Kinugasa Sanpo, 390, pp.13~21 (1954)
- 정종훈, "Enhancement of 1-Deoxynojirimycin Content and α-Glucosidase Inhibitory Activity in Mulberry Leaf Using Various Fermenting Microorganisms Isolated from Korean Traditional Fermented Food", Biotechnology and Bioprocess Engineering, 19(6), pp.1114~1118 (2014), 한국생물공학회
- Kalpana D, "Assessment of genetic diversity among varieties of mulberry using RAPD and ISSR fingerprinting", Sci Hortic, 134, pp.79~87 (2012)
- Kapche GD, "Prenylated arylbenzofuran derivatives from Morus mesozygia with antioxidant activity", Phytochemistry, 70, pp.216~221 (2009)
- Koidzumi G, "Taxonomical discussion on Morus plants", Bull Imp Sericult Exp Stat, 3, pp.1~62 (1917)
- Kumar S, "MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets", Mol Biol Evolution, 33, pp.1870~1874 (2016)
- Leroy JF, "Les Muriers sauvages et cultives. La sericiculture sous les tropiques Rev", J Agric Tradit Bot Appl, 29, pp.481~496 (1949)
- Li DZ, "Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants", Proc Natl Acad Sci USA, 108, pp.19641~19646 (2011)
- Linnaeus C, "Morus (In Species Plantarum Vol. 2)", pp.968~, Impensis Laurentii Salvii
- Nepal MP, "Systematics and reproductive biology of the genus Morus L.(Moraceae).", Ph.D., pp.2008
- Nepal MP, "Phylogenetics of Morus (Moraceae)Inferred from ITS and trnL-trnF Sequence Data", Syst Bot, 37, pp.442~450 (2012)
- Pawlowska AM, "Quali-quantitative analyses of flavonoids of Morus nigra L. and Morus alba L.(Moraceae) fruits", J Agric Food Chem, 56, pp.3377~3380 (2008)
- Rao DMR, "Scope of pre-breeding in mulberry crop improvement–A review", Sci Wkly, 1, pp.1~18 (2013)
- Saitou N, "The neighbor-joining method: A new method for reconstructing phylogenetic trees", Mol Biol Evol, 4, pp.406~425 (1987)
- Schneider CK, "Plantae Wilsonianae: an enumeration of the woody plants collected in western China for the Arnold arboretum of Harvard university during the years 1907, 1908, and 1910", pp.293~294 (1917), The University Press
- Sharma A, "Assessment of genetic diversity in a Morus germplasm collection using fluorescence-based AFLP markers", Theor Appl Genet, 101, pp.1049~1055 (2000)
- Vijayan K, "Molecular characterization and identification of markers associated with yield traits in mulberry using ISSR markers", Plant Breeding, 125, pp.298~301 (2006)
- Zeng Q, "Definition of Eight Mulberry Species in the Genus Morus by Internal Transcribed Spacer-Based Phylogeny", PLoS ONE, 10, pp.e013541~ (2015)
- Zhang SD, "Multi-gene analysis provides a well-supported phylogeny of Rosales", Mol Phylogenet Evol, 60, pp.21~28 (2011)
- Zhao W, "Isolation and characterization of microsatellite loci from the mulberry", Morus L. Plant Sci, 168, pp.519~525 (2005)
- Zhao WG, "Phylogenetic relationship of genus Morus by ITS sequence data", Sci Seric, 30, pp.11~14 (2004)
- Zheng ZP, "Tyrosinase inhibition constituents from the roots of Morus australis", Fitoterapia, 83, pp.1008~1013 (2012)
- Zhu B, "Enterobacter mori sp. nov., associated with bacterial wilt on Morus alba L", Int J Syst Evol Microbiol, 61, pp.2769~2774 (2011)