Internal Transcribed Spacer (ITS) Regions Reveals Phylogenic Relationships of Rubus Species Cultivated in Korea
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초록
The bramble cultivated widely in South Korea, which is known as Bokbunja-ddal-gi, is regarded as having originated from Korean native Rubus coreanus. This study was carried out to obtain basic phylogenetic information on Korean cultivated bramble (KCB) by comparing the internal transcribed spacer (ITS) regions with those of R. coreanus, blackberry (R. lanciniatus), black (R. occidentalis) and red (R. idaeus) raspberry. Sequences of the ITS 1 suggest that some KCB accessions share a significant similarity with both R. occidentalis and R. coreanus in the ITS 1 region. The ITS 2 sequences of the three KCB accessions clustered more closely to those of two R. occidentalis accessions than to those of R. coreanus. These results suggest that there exist variations in the sequences of ITS among KCB accessions and KCB accessions are more closely related to black raspberry than R. coreanus in the ITS regions.
Abstract
The bramble cultivated widely in South Korea, which is known as Bokbunja-ddal-gi, is regarded as having originated from Korean native Rubus coreanus. This study was carried out to obtain basic phylogenetic information on Korean cultivated bramble (KCB) by comparing the internal transcribed spacer (ITS) regions with those of R. coreanus, blackberry (R. lanciniatus), black (R. occidentalis) and red (R. idaeus) raspberry. Sequences of the ITS 1 suggest that some KCB accessions share a significant similarity with both R. occidentalis and R. coreanus in the ITS 1 region. The ITS 2 sequences of the three KCB accessions clustered more closely to those of two R. occidentalis accessions than to those of R. coreanus. These results suggest that there exist variations in the sequences of ITS among KCB accessions and KCB accessions are more closely related to black raspberry than R. coreanus in the ITS regions.
참고문헌 (16)
- Alice LA, "Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA", American Journal of Botany, 86, pp.81~97 (1999)
- Altschul SF, "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Research, 25, pp.3389~3402 (1997)
- Baldwin BG, "Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositae", Molecular Phylogenetics and Evolution, 1, pp.3~16 (1992)
- Baldwin BG, "The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny", Annals of the Missouri Botanical Garden, 82, pp.247~277 (1995)
- Brodsky LI, "GeneBee-NET: Internet-based server for analyzing biopolymers structure", Biochemistry, 60, pp.923~928 (1995)
- Clevinger JA, "Phylogenetic analysis of Silphium and subtribe Engelmanniiae (Asteraceae: Heliantheae) based on ITS and ETS sequence data", American Journal of Botany, 87, pp.565~572 (2000)
- Eriksson T, "Phylogenetic analysis of Potentilla using DNA sequence of nuclear ribosomal the internal transcribed spacers (ITS) and implications for the classification of Rosoideae (Rosaceae)", Plant Systematics and Evolution, 211, pp.155~179 (1998)
- 유기석, "Phylogenic Relationships of Rubus Species Revealed by Randomly Amplified Polymorphic DNA Markers", Journal of Crop Science and Biotechnology, 11(1), pp.39~44 (2008), 한국작물학회
- Gilbert DC, "SeqApp 1.9. A biological sequence editor and analysis program for Macintosh computers", Available at http:, ftp.indiana.edu, pp.1994, ~
- Hsiao C, "Phylogenetic relationships of ten grass species: an assessment of phylogenetic utility of the internal transcribed spacer region in the nuclear ribosomal DNA in monocots", Genome, 37, pp.112~120 (1994)
- Jeong JS, "Encyclopedia of oriental medical", pp.461~ (1996), Young Rim Publishing Company Limited
- Kim YD, "Phylogeny of Weigela and Diervilla (Caprifoliaceae) based on nuclear rDNA ITS sequence: Biogeographic and taxonomic implications", Journal of Plant Research, 112, pp.331~341 (1999)
- 이현용, "초음파 병행 추출이 마황과 복분자 열수 추출물의 면역활성 증진에 미치는 효과", KSBB Journal, 19(2), pp.113~117 (2004), 한국생물공학회
- Pillay M, "Genetic diversity in cotton assessed by variation in ribosomal RNA genes and AFLP markers", Crop Science, 39, pp.1881~1886 (1999)
- Stanford AM, "Phylogeny and Biogeography of Juglans (Juglandaceae) based on matK and ITS sequence", American Journal of Botany, 87, pp.872~882 (2000)
- White TJ, "Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols. A Guide to Methods and Applications", pp.315~322 (1990), Academic Press