한국특산종 외대잔대(Adenophora racemosa)의 분자계통학적 위치
Molecular Phylogenetic Position of Adenophora racemosa, an Endemic Species in Korea
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Adenophora racemosa is recently reported as a new Korean endemic plant species. However, the phylogenetic relationship of this genus has been controversial due to the morphological similarity and frequent morphological change of aerial parts. To verify the phylogenetic position of Adenophora racemosa and phylogenetic relationship of genus Adenophora,we analyzed the internal transcribed spacer (ITS) sequence of nuclear ribosomal DNA (nrDNA) and random amplified polymorphic DNA (RAPD) using 21 individual of 6 Adenophora species, A. verticillata, A. divaricata, A. racemosa, A. remotiflora,A. stricata and A. tetraphylla. In comparative analysis of the nrDNA-ITS sequences, we could not found not only any species specific nucleotide sequence but also could not estimated their inter or intra species. In the phylogenic analysis based on the RAPD derived DNA polymorphism, Adenophora species were classified into four groups by clustering analysis of the UPGMA. These results suggest that the DNA fingerprinting based on RAPD is more suitable than nrDNA-ITS sequence for the phylogenetic analysis of Adenophora species
Abstract
Adenophora racemosa is recently reported as a new Korean endemic plant species. However, the phylogenetic relationship of this genus has been controversial due to the morphological similarity and frequent morphological change of aerial parts. To verify the phylogenetic position of Adenophora racemosa and phylogenetic relationship of genus Adenophora,we analyzed the internal transcribed spacer (ITS) sequence of nuclear ribosomal DNA (nrDNA) and random amplified polymorphic DNA (RAPD) using 21 individual of 6 Adenophora species, A. verticillata, A. divaricata, A. racemosa, A. remotiflora,A. stricata and A. tetraphylla. In comparative analysis of the nrDNA-ITS sequences, we could not found not only any species specific nucleotide sequence but also could not estimated their inter or intra species. In the phylogenic analysis based on the RAPD derived DNA polymorphism, Adenophora species were classified into four groups by clustering analysis of the UPGMA. These results suggest that the DNA fingerprinting based on RAPD is more suitable than nrDNA-ITS sequence for the phylogenetic analysis of Adenophora species
참고문헌 (39)
- Cooperation teaching materials compilation committee of oriental medicine college in Korea, "Herbal Medicine", pp.644~645 (2005), Younglimsa Press
- 유기석, "Internal Transcribed Spacer (ITS) Regions Reveals Phylogenic Relationships of Rubus Species Cultivated in Korea", 한국약용작물학회지, 17(3), pp.165~172 (2009), 한국약용작물학회
- Ghada B, "Genetic analysis of tunisian fig (Ficus carica L.) cultivars using amplified fragment length polymorphism (AFLP) markers", Scientia Horticulturae, 120, pp.487~492 (2009)
- Han JP, "Genetic diversity and biogeography of the traditional chinese medicine,Gardenia jasminoides,based on AFLP markers", Biochemical Systematics and Ecology, 35, pp.138~145 (2007)
- 배갈마, "염기서열을 이용한 한약재 형개의 기원 및 유연관계 분석", 한국약용작물학회지, 17(1), pp.46~53 (2009), 한국약용작물학회
- Kress WJ, "Use of DNA barcodes to identify flowering plants", Proceedings of the National Academy of Sciences, 102, pp.8369~8374 (2005)
- Kim CK, "Genetic diversity and population structure of endangered Isoetes coreana in south Korea based on RAPD analysis", Aquatic Botany, 89, pp.43~49 (2008)
- Kim IS, "Genetic diversity of Abies koreana Wilson based on RAPD analysis", Korean Journal of Breeding, 32, pp.12~18 (2000)
- Kim MY, "Korean Endemic Plant", pp.160~162 (2004), Solkwahak
- Kim OT, "Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences", Plant Biotechnol Report, 1, pp.163~167 (2007)
- 김영동, "제주도 황근(Hibiscus hamabo) 집단의 유전적 다양성", 식물분류학회지, 37(2), pp.115~129 (2007), 한국식물분류학회
- 이정훈, "현삼속 식물의 종판별을 위한 Mitochondrial DNA의 염기서열 및 PCR-RFLP 분석", 한국약용작물학회지, 18(3), pp.173~179 (2010), 한국약용작물학회
- Lee JK, "Taxonomy of the genus Adenophora (Adenophorae) in Korea", pp.75~ (1989), Sungkyunkwan University
- Lee JK, "Adenophora racemosa(Campanulaceae),a new species from Korea", Journal of Korean Plant Taxonomy, 20, pp.121~126 (1990)
- 이재순, "Development of Nuclear Ribosomal ITS DNA Marker for Tricholoma matsutake", 한국임학회지, 93(2), pp.121~127 (2004), 한국임학회
- Lee TB, "Coloured Flora of Korea (V. 2)", pp.681~ (2006), Hyangmunsa Press
- 이선근, "Rhizina undulata RDNA ITS 영역의 PCR 검정 및 염기배열 분석", 한국임학회지, 96(4), pp.425~431 (2007), 한국임학회
- 이상용, "리지나뿌리썩음병균 분리주들의 배양 특성 및 RAPD에 의한 유전적 다양성 분석", 한국임학회지, 95(4), pp.388~392 (2006), 한국임학회
- Lin WY, "Rapid authentication of Bupleurum species using an array of immobilized sequencespecific oligonucleotide probes", Planta Medica, 74, pp.464~469 (2008)
- Li UM, "High genetic differentiation revealed by RAPD analysis of narrowly endemic Sinocalycanthus chinensis Cheng et S.Y.Chang,an endangered species of China", Biochemical Systematics and Ecology, 34, pp.725~735 (2006)
- 박종욱, "한국산 여뀌속 Persicaria절(마디풀과)의 핵 리보오솜 ITS 염기서열 변이", 식물분류학회지, 36(1), pp.21~40 (2006), 한국식물분류학회
- 조용구, "쑥 수집종의 형태적 형질과 RAPD분석에 의한 분류", 한국약용작물학회지, 13(6), pp.278~286 (2005), 한국약용작물학회
- 박선주, "호랑가시나무(Ilex cornuta) 개체군의 ITS 염기서열 변이 분석", 식물분류학회지, 37(2), pp.131~141 (2007), 한국식물분류학회
- Sreekumar VB, "Assessment of genetic diversity in Calamus thwaitesii BECC.(Arecaceae)using RAPD markers", Biochemical Systematics and Ecology, 34, pp.397~405 (2006)
- Sucher NJ, "Genome-based approaches to the authentication of medicinal plants", Planta Medica, 74, pp.603~623 (2008)
- Vos P, "AFLP:a new technique for DNA fingerprinting", Nucleic Acids Research, 23, pp.4407~4421 (1995)
- Weising K, "DNA fingerprinting in plants", pp.21~146 (2005), CRC press
- White, TM, "Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics", PCR protocols a guide to methods and applications, pp.315~322 (1990)
- Williams JG, "DNA polymorphisms amplified by arbitrary primers are useful as genetic markers", Nucleic Acids Research, 18, pp.6531~6535 (1990)
- Xie H, "Identification of crude drugs from chinese medicinal plants of the genus Bupleurum using ribosomal DNA ITS sequences", Planta Medica, 75, pp.89~93 (2009)
- Xu H, "Differentiation of Dendrobium species used as"Huangcao Shihu"by rDNA ITS sequence analysis", Planta Medica, 72, pp.89~92 (2006)
- Yang DY, "Molecular analysis of Rheum species used as Rhei Rhizoma based on the chloroplast matK gene sequence and its application for identification", Biological & Pharmaceutical Bulletin, 4, pp.375~383 (2004)
- Yoo KO, "A palynotaxonomic study of the Korean Campanulaceae", pp.278~ (1995), Kangwon National University
- 유기억, "엽록체 DNA의 matK와 atpB-rbcL 염기서열 분석에 의한 제비꽃속(Viola)의 계통유연관계", 식물분류학회지, 37(1), pp.1~15 (2007), 한국식물분류학회
- Yoo KO, "Comparative studies on the Hanabusaya asiatica and its allied groups based on randomly amplified polymorphic DNA(RAPD)analysis", Horticulture,Environment and Biotechnology, 37, pp.324~328 (1996)
- Zabeau, M, "Selective restriction fragment amplification: a general method for DNA fingerprinting" (1993), European Patent Office. Munich
- Zhao NA, "RAPD diversity of Stipa grandis populations and its relationship with some ecological factors", Acta Ecologica Sinica, 26, pp.1312~1318 (2006)
- Zheng DJ, "RAPD analysis of germplasm resources of Kudingcha species in Oleaceae", Agricultural Sciences in China, 8, pp.784~792 (2009)
- Zietkiewicz E, "Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification", Genomics, 20, pp.176~183 (1994)