Brassica 속 식물 내 종간교잡에 따른 교잡효율 및 F1잡종의 표현형질
The Cross Ability and the Phenotypic Characteristics of F1 Hybrid in the Interspecific Crosses between Brassica napus and B. campestris, B. rapa
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
본 연구는 유채-MS를 이용하여 배추와 순무 등을 화분친으로 활용 잎의 모용이 없는 유채 잎에 배추와 순무의 엽색과 엽형을 도입하여 소비자가 선호하는 새로운 형질을 갖춘 엽채소의작출 가능성을 검토하고자 실시하였다. 교배효율에 영향을 미치는 인공수분 후 주두 위에서의 화분발아나 화분관 신장은 유채-MS와 배추간 종간 교배 조합에서 유채-MS와 순무간 종간교잡보다 빠르게 진행되었으며, 유채-MS와 배추간 종간교잡에서의 결협율이 90.6%로 유채-MS와 순무의 교배 결협율67.3% 보다 23.3% 정도 높게 나타났으며, 협당결실립수는 각각15.5립, 11.6립으로 나타나 유채-MS와 배추간의 종간교잡 효율이 높았다. 유채-MS와 배추의 종간교잡 F1 잡종의 표현형질의 특성은 주로 모계인 유채와 비슷하였으며 엽색은 밝은 녹색으로 양친의 중간형을 나타냈고 모용은 적게 분포되어 있었으며 엽수는 10.2매, 엽장은 27.5 ㎝, 엽폭은 14.0 ㎝로 잎의 길이와 넓이가 유채에 비하여 커졌다. 유채-MS와 순무의 종간교잡F1 잡종의 엽수는 9.6매, 엽장은 21.6 ㎝, 엽폭은 10.6 ㎝로 잎의크기, 형태 및 결각 등은 양친의 중간형을 나타냈으며 엽색은 모본인 유채와 비슷하거나 좀 더 짙은 녹색이었고, 모용이 잎의 앞과 뒷면에 존재하였다.
Abstract
Interspecific crosses between rapeseed (Brassica napus L.) and chinese cabbage (B. campestris var. pekinensisMakino) and turnip (B. rapa L.) were made in order to examine the cross possibility and morphological phenotype of F1hybrid. The growth of pollen tube of cross between rapeseed and chinese cabbage was more rapid than cross betweenrapeseed and turnip. Silique formation and seed setting in silique were relatively high in the cross between rapeseed andchinese cabbage. The percentage of silique set from interspecific cross between rapeseed and chinese cabbage was 90.6%and higher 23.3% than the percentage of silique from interspecific cross rapeseed and turnip. The average number of seedper silique obtained from the cross rapeseed and chinese cabbage, and rapeseed and turnip reached 15.5 and 11.6,respectively. The morphological phenotypes of F1 hybrid plants obtained from seeds in the cross between rapeseed andchinese cabbage resembled rapeseed mainly, but leaf length and leaf width were increased. The size, shape and lobation ofleaves of F1 hybrid plants from interspecific cross between rapeseed and turnip were intermediated between their parentspecies, but color of leaves was dark-green.
참고문헌 (27)
- Aslam-Yousuf, M, "Morphology and cytology of hybrids between Brassica napus and B. campestris", Pakistan J. Agric. Res, 5, pp.11~17 (1984)
- Ahn, C.S, "Interspcific and intergeneric cross of Brassica juncea (L.) Czern and Coss. with B. campestris L. ssp. pekinensis and Raphanus sativus L", J. Kor. Soc. Hort. Sci, 27, pp.305~313 (1986)
- Ayotte, R, "The transfer of trizane resistance from Brassica napus L. to B. oleracea L. Ⅰ. Production of F1 hybrids through embryo rescue", Euphytica, 36, pp.615~624 (1987)
- Cartea, M.E, "Phenolic compounds in Brassica vegetables", Molecules, 16, pp.251~280 (2011)
- Chang, C.T, "The effect of the cytoplasms of Brassica napus and B. juncea on some characteristics of B. carinata, including flower morphology", Euphytica, 158, pp.261~270 (2007)
- Chen, J.P, "Synthesis and characterization of interspecific trigenomic hybrids and allohexaploids between three cultivated Brassica allotetraploids and wild species Brassica fruticulosa", African Journal of Biotechnology, 10, pp.12171~12176 (2011)
- Chiang, M.S, "Transfer of resistance race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea var. capitata).Ⅰ.Interspecific hybridization between B. napus and B. oleracea var. capitata", Euphytica, 26, pp.319~336 (1977)
- Chiang, M.S, "Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to common cabbage : In Talekar, N. S. and T. D. Griggs(eds. ), Chinese Cabbage, Proc. First International Symp., Tokyo, Japan. pp", Chinese Cabbage, Proc. First International Symp, pp.415~422 (1981)
- Gowers, S, "The transfer of characters from Brassica campestris L. to Brassica napus L.: Production of clubrootresistant oil-seed rape (B. napus ssp. oleifera)", Euphytica, 31, pp.971~976 (1982)
- Gul, H, "Effect of salinity on pollen viability of different canolra (B. napus L.) cultivars as reflected by the formation of fruits and seeds", Pak. J. Bot, 38, pp.237~247 (2006)
- Hu, Q, "Intergeneric hybrids between Brassica napus and Orychophragmus violaceus containing traits of agronomic importance for oilseed rape breeding", Theor. Appl. Genet, 105, pp.834~840 (2002)
- Inomata, N, "Culture in vitro of excised ovaries in Brassica campestris L. I. Development of excised ovaries in culture media, temperature and light", Jap. J. Breed, 26, pp.229~236 (1976)
- Kapusta-Duch, J, "The beneficial effects of Brassica vegetables on human health", Rocz. Panstw. Zakl. Hig, 63, pp.389~395 (2012)
- 이태성, "파종시기에 따른 유채(Brassica napus L.) 품종별 생육 및 개화특성", 한국자원식물학회지, 27(1), pp.80~88 (2014), 한국자원식물학회[DOI]
- 이태성, "파종시기에 따른 유채(Brassica napus L.) 기름함량 및 지방산 조성 특성", 한국자원식물학회지, 27(2), pp.202~208 (2014), 한국자원식물학회[DOI]
- Malek, M.A, "Study of crossability and F1 of interspecific hybridization between Brassica rapa (B. campestris) and B. nigra", Bangladesh J. Agril. Res, 32, pp.445~449 (2007)
- Outcrossing potential for Brassica species and implications for vegetable crucifer seed crops of growing oilseed Brassicas in the Willamette valley, "Myers, J.R", Oregon State University Extension, ~, pp.Service Special Report 1064 (2006)
- Ollson, G, "Polyploid breeding in Europe: In Tsunoda et al. (eds.), Brassica crops and wild allies", pp.167~190 (1980), Jap. Sci. Soc. Press
- Ozminkowski, R.H, "Comparing the resynthesis of Brassica napus L. by interspecific somatic and sexual hybridization. II. Hybrid morphology and identifying organelle genomes", J. Amer. Soc. Hort. Sci, 119, pp.816~823 (1994)
- Paul, E.M, "Gene dispersal from genetically modified oilseed rape in the field", Euphytica, 81, pp.283~289 (1995)
- Pramod, K, "Studies on pollen-pistil interaction in intergeneric and interspecific crosses of oilseed Brassica’s and related genera", An International Journal of Plant Research, 25, pp.276~281 (2012)
- Shivanna, K.R, "Pollen biotechnology for crop production and improvement", pp.261~272 (1997), Cambridge University Press
- Snowdon, R.J, "Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids", Theor. Appl. Genet, 95, pp.1320~1324 (1997)
- U. N, "Genome-analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization", Jap. J. Bot, 7, pp.977~981 (1935)
- Weerakoon, S. R, "Producing inter-specific hybrids between Brassica juncea (L.) Czern & Coss and B. oleracea (L.) to synthesize trigenomic (ABC) Brassica", J. Sci. Univ. Kelaniya, 6, pp.13~34 (2011)
- Zhang, G.Q, "Resynthesizing Brassica napus from interspecific hybridization between Brassica rapa and B. oleracea through ovary culture", Euphytica, 140, pp.181~187 (2004)
- Zhao, Z.G, "Production and characterization of intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus and their backcrossing progenies", Plant Cell Rep, 27, pp.1611~1621 (2008)