배추 종간 잡종의 소포자배양에 의한 Double haploid 집단의 플라보노이드 함량 분석
Analysis of flavonoids in double haploid population derived from microspore culture of F1 hybrid of Brassica rapa
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One of the most important species, Brassica rapa encompasses a variety of commercial vegetables, such as the Chinese cabbage, pak choi and oilseed crops. The LP08 of yellow sarson (Brassica rapa ssp, tricolaris) have a distinct morphology, with yellow seed color and a unique tetralocular ovary. LP21 of pak choi (Brassica rapa ssp, chinensis) have a dark brown seed color and bilocular ovary. In this study, we generated double haploid plants by crossing the LP08 (maternal variety) and LP21 (paternal variety), using microspore culture. A total of 66 accessions with various morphological characteristics were used for content analysis of flavonoids. The three flavonoids, quercetin, naringenin and kaempferol, showed differing contents in the two crossing parents. The Chinese cabbage type 'Chiifu' was used as the control. The highest accumulation of total flavonoids was observed in LP08. The lowest mean total flavonoids were found in 'Chiifu'. Among the 66 DH accessions, the quercetin contents of 18 accessions showed higher content than LP08. Kaempferol content was also high, and was found to be 79.7% of the total flavonoid content. Naringenin content was low at 2.8%, and was not detected in 22 accessions. Interestingly, the quercetin content positively correlated with the kaempferol content. These results can be used to identify genetic locus and genes related to useful traits. Phenotypic analysis of 66 DH accessions can further be used for natural selection of good breeding materials in B. rapa.
Abstract
One of the most important species, Brassica rapa encompasses a variety of commercial vegetables, such as the Chinese cabbage, pak choi and oilseed crops. The LP08 of yellow sarson (Brassica rapa ssp, tricolaris) have a distinct morphology, with yellow seed color and a unique tetralocular ovary. LP21 of pak choi (Brassica rapa ssp, chinensis) have a dark brown seed color and bilocular ovary. In this study, we generated double haploid plants by crossing the LP08 (maternal variety) and LP21 (paternal variety), using microspore culture. A total of 66 accessions with various morphological characteristics were used for content analysis of flavonoids. The three flavonoids, quercetin, naringenin and kaempferol, showed differing contents in the two crossing parents. The Chinese cabbage type 'Chiifu' was used as the control. The highest accumulation of total flavonoids was observed in LP08. The lowest mean total flavonoids were found in 'Chiifu'. Among the 66 DH accessions, the quercetin contents of 18 accessions showed higher content than LP08. Kaempferol content was also high, and was found to be 79.7% of the total flavonoid content. Naringenin content was low at 2.8%, and was not detected in 22 accessions. Interestingly, the quercetin content positively correlated with the kaempferol content. These results can be used to identify genetic locus and genes related to useful traits. Phenotypic analysis of 66 DH accessions can further be used for natural selection of good breeding materials in B. rapa.
참고문헌 (23)
- Burnett, L., "Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L ssp. oleifera", Plant Cell Rep, 11, pp.215~218 (1992)
- Cao MQ, "Embryogenesis and plant regeneration of pakchoi (Brassica rapa L. ssp. chinensis) via in vitro isolated microspore culture", Plant Cell Rep, 13, pp.447~450 (1994)
- Cartea ME, "Phenolic compounds in Brassica vegetables", Molecules, 16, pp.251~280 (2011)
- Chung HY, "The molecular inflammatory process in aging", Antioxid Redox Sign, 8, pp.572~581 (2006)
- "CoGePEDIA_Sequenced Plant Genome Website"[URL]
- Harbaum B, "Identification of flavonoids and hydroxycinnamic acids in pak choi varieties (Brassica campestris L. ssp. chinensis var. communis) by HPLC-ESI-MSn and NMR and their quantification by HPLC-DAD", J Agric Food Chem, 55, pp.8251~8260 (2007)
- Harbaum B, "Impact of fermentation on phenolic compounds in leaves of pak choi (Brassica campestris L. ssp. chinensis var. communis) and Chinese leaf mustard (Brassica juncea Coss)", J Agric Food Chem, 56, pp.148~157 (2008)
- Hertog MGL, "Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9fruits commonly consumed in the Netherlands", J Agric Food Chem, 40, pp.2379~2383 (1992)
- Holden JM, "Development of a database of critically evaluated flavonoids data: application of USDA’s data quality evaluation system", J Food Compost Anal, 18, pp.829~844 (2005)
- Jacobs DR, "Whole grain intake and cancer: A review of the literature", Nutr Cancer, 24, pp.221~229 (1995)
- Koes R, "Flavonoids: A colorful model for the regulation and evolution of biochemical pathways", Trends Plant Sci, 10, pp.236~242 (2005)
- Krumbein A, "Changes in quercetin and kaempferol concentrations during broccoli head ontogeny in three brokkoli cultivars", J appl bot food qual, 81, pp.136~139 (2007)
- Murtaza I, "Antioxidant activity and total phenolic content of kale genotypes grown in Kashmir vally", J plant biochem biot, 14, pp.215~217 (2005)
- Olsen H, "Characterization and quantification of flavonoids and hydroxycinnamic acids in curly kale(Brassica oleracea L. convar. acephala var. sabellica) by HPLC-DAD-ESI-MSn", J Agric Food Chem, 57, pp.2816~2825 (2009)
- Podsędek A, "Natural antioxidants and antioxidant capacity of Brassica vegetables: A review", Lwt-Food Sci Technol, 40, pp.1~11 (2007)
- by HPLCDAD and LC-MS, "MS", J Agric Food Chem, 54, pp.4655~4860 (2006)
- Routaboul JM, "Metabolite profiling and quantitative genetics of natural variation for flavonoids in Arabidopsis", J exp Bot, 63, pp.3749~3764 (2012)
- Schmidt S, "Genotypic and climatic influences on the concentration and composition of flavonoids in kale(Brassica oleracea var. sabellica)", Food Chem, 119, pp.1293~1299 (2010)
- 서미숙, "배추 유전자원의 소포자 유래 배 발생 효율에 미치는 배양 조건 구명", Journal of Plant Biotechnology, 41(3), pp.116~122 (2014), 한국식물생명공학회
- 서미숙, "기내배양을 통한 Brassica 작물의 반수체 생산에 관한 연구 동향", 한국국제농업개발학회지, 27(4), pp.522~528 (2015), 한국국제농업개발학회[DOI]
- Truswell AS, "Cereal grains and coronary heart disease", Eur J Clin Nutr, 56, pp.1~14 (2002)
- Vallejo F, "Characterisation of flavonols in broccoli (Brassica oleracea L. var. italica) by liquid chromatography-UV diode-array detection-electrospray ionization mass spectrometry", J Chromatogr, 1054, pp.181~193 (2004)
- Wang XW, "The genome of the mesopolyploid crop species Brassica rapa", Nat Genet, 43, pp.1035~U1157 (2011)