Immune Cells Activity, Nitrite Scanvenging and ABTS Radical Scavenging Activities of Codonopsis lanceolata Ethanol Extracts from Districts in Korea
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
This study was executed to evaluate the immune activity, nitrite scavenging activity and ABTS radical scavenging activity against extracts of various concentration of ethanol solvent from Codonopsis lanceolata cultured at 6 local regions. The immune responses from both human T and B cell line was significantly enhanced in the cell growth compared to control while the cell growth was influenced at a certain period of culture. The results revealed that the cell growth of both human T and B cell was altered in a time dependent manner. The nitrite scavenging activity of ethanol extracts from various solvent concentration of C. lanceolata were affected by pH. At a pH of 1.2, the nitrite scavenging effect of all of the extracts tested observed higher than that of the other two pH ranges. There was no distinct detection of nitrite scavenging effects of the pH range 6.0. The ABTS radical scavenging activity was progressively increased in a dose-dependent manner, and the activity was the highest in 100% ethanol extract. The result from this investigation suggests that the extract of Codonopsis lanceolata could be an addition to basic medicine for immune modulation and natural food additives. Key words - Immune activity, Nitrite Scavenging Activity, ABTS radical scavenging activity, Codonopsis lanceolata
Abstract
This study was executed to evaluate the immune activity, nitrite scavenging activity and ABTS radical scavenging activity against extracts of various concentration of ethanol solvent from Codonopsis lanceolata cultured at 6 local regions. The immune responses from both human T and B cell line was significantly enhanced in the cell growth compared to control while the cell growth was influenced at a certain period of culture. The results revealed that the cell growth of both human T and B cell was altered in a time dependent manner. The nitrite scavenging activity of ethanol extracts from various solvent concentration of C. lanceolata were affected by pH. At a pH of 1.2, the nitrite scavenging effect of all of the extracts tested observed higher than that of the other two pH ranges. There was no distinct detection of nitrite scavenging effects of the pH range 6.0. The ABTS radical scavenging activity was progressively increased in a dose-dependent manner, and the activity was the highest in 100% ethanol extract. The result from this investigation suggests that the extract of Codonopsis lanceolata could be an addition to basic medicine for immune modulation and natural food additives. Key words - Immune activity, Nitrite Scavenging Activity, ABTS radical scavenging activity, Codonopsis lanceolata
참고문헌 (19)
- Boora, F., "Evaluation of Nitrite Radical Scavenging Properties of Selected Zimbabwean Plant Extracts and Their Phytoconstituents", J. Food Process (2014)
- Buckley, R. H., "Hematopoietic stem-cell transplantation for the treatment of severe combined immunodeficiency", New Engl. J. Med, 340, pp.508~516 (1999)
- DuBok Choi, "Effect of bamboo oil on antioxidative activity and nitrite scavenging activity", Journal of Industrial and Engineering Chemistry, 14(6), pp.765~770 (2008), 한국공업화학회
- Foyer, C. H., "Protection against oxygen radicals : an important defence mechanism studied in transgenic plants", Plant Cell Env, 17, pp.507~523 (1994)
- Guizani, N., "Nabag(Zizyphus spina-christi)extract prevents aberrant crypt foci development in colons of azoxymethane-treated rats by abrogating oxidative stress and inducing apoptosis", Asian Pac J Cancer Prev, 14, pp.5031~5035 (2013)
- 임무현, "솔잎발효추출물의 효소적 저해활성 및 아질산염 소거작용", 한국식품저장유통학회지, 11(1), pp.94~99 (2004), 한국식품저장유통학회
- Isaykina, N., "Immunotropic activity of plant extract echinosol", B. Exp. Biol. Med, 146, pp.223~225 (2008)
- Janeway, C. A., "Immunobiology : the immune system in health and disease" (1999), Current Biology Publications New York
- Joh, E. H., "Lancemaside A ameliorates colitis by inhibiting NF-jB activation in TNBS-induced colitis mice", Int. J. Colorectal Dis, 25, pp.545~551 (2010)
- 강미영, "우슬과 골쇄보의 추출물이 항산화 활성 및 항산화 효소 대사에 미치는 효과", 한국자원식물학회지, 28(5), pp.600~607 (2015), 한국자원식물학회[DOI]
- Kato, H., "Inhibition of nitrosamine formation by nondialyzable melanoidins", Agr. Biol. Chem, 51, pp.1333~1338 (1987)
- Lee, J. Y., "Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum De candolle roots", J. Ethnopharmacol, 93, pp.409~415 (2004)
- 노보영, "Antioxidant and ACE Inhibitory Activity of Cultivated and Wild Angelica gigas Nakai Extracts Prepared Using Different Extraction Conditions", Preventive Nutrition and Food Science, 19(4), pp.274~280 (2014), 한국식품영양과학회[DOI]
- 박윤점, "쪽 종자의 영양성분과 메탄올 추출물의 생리활성 효과", 한국자원식물학회지, 29(1), pp.32~38 (2016), 한국자원식물학회[DOI]
- Paul, D. J., "Antiproliferative and antimicrobial activities of alkylbenzoquinone derivatives from Ardisia kivuensis", Pharm. Biol, 52 (2014)
- Pittella, F., "Antioxidant and cytotoxic activities of Centella asiatica(L)Urb", Int. J. Mol. Sci, 10, pp.3713~3721 (2009)
- Re, R., "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Bio. Med, 26(9-10) (1999)
- 성낙주, "유자의 화학적 특성 및 아질산염 소거작용", 한국식품영양과학회지, 34(4), pp.496~502 (2005), 한국식품영양과학회
- Wang, L., "Codonopsis lanceolata extract induces G0", G1 arrest and apoptosis in human colon tumor HT-29 cells-Involvement of ROS generation and polyamine depletion, 2011, pp.49, 149~154