In Vitro Cytotoxicity against Human Cancer Cell and 3T3-L1 Cell, Total Polyphenol Content and DPPH Radical Scavenging of Codonopsis lanceolata according to the Concentration of Ethanol Solvent
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This study was executed to evaluate the phenolic content, DPPH radical scavenging rate, and the cytotoxic effect in human cancer cell, 3T3-L1 cell from C. lanceolata extracts at various ethanol concentration. Total polyphenol and flavonoid content of the C. lanceolata at various ethanol concentration showed the high amount in 70%, 100% ethanol extract. The DPPH radical scavenging activity progressively increased in a dose-dependent manner, and showed the highest in 100% ethanol extract. The cytotoxic effect against human cancer cell of the C. lanceolata was higher in 50% and 70% ethanol extracts. In particular, the cytotoxic effect in MCF-7 cell was relatively higher than in other cells. The IC50 (concentration causing 50% cell death) value showed the highest on MCF-7 cell (538.39 ㎍/㎖) in 70% ethanol extract, and exhibited significant activity against Hela cell (637.87 ㎍/㎖), Calu-6 cell (728.64 ㎍/㎖). The extract of 70% ethanol at 1,000 ㎍/㎖ exhibited a pronounced cytotoxic effect on 3T3-L1 cell comparable to that of the other extracts, and reduced in a concentration-dependent manner. Key words – Codonopsis lanceolate, Cytotoxicity, DPPH radical, Flavonoid, Polyphenol, 3T3-L1 preadipocyte cell
Abstract
This study was executed to evaluate the phenolic content, DPPH radical scavenging rate, and the cytotoxic effect in human cancer cell, 3T3-L1 cell from C. lanceolata extracts at various ethanol concentration. Total polyphenol and flavonoid content of the C. lanceolata at various ethanol concentration showed the high amount in 70%, 100% ethanol extract. The DPPH radical scavenging activity progressively increased in a dose-dependent manner, and showed the highest in 100% ethanol extract. The cytotoxic effect against human cancer cell of the C. lanceolata was higher in 50% and 70% ethanol extracts. In particular, the cytotoxic effect in MCF-7 cell was relatively higher than in other cells. The IC50 (concentration causing 50% cell death) value showed the highest on MCF-7 cell (538.39 ㎍/㎖) in 70% ethanol extract, and exhibited significant activity against Hela cell (637.87 ㎍/㎖), Calu-6 cell (728.64 ㎍/㎖). The extract of 70% ethanol at 1,000 ㎍/㎖ exhibited a pronounced cytotoxic effect on 3T3-L1 cell comparable to that of the other extracts, and reduced in a concentration-dependent manner. Key words – Codonopsis lanceolate, Cytotoxicity, DPPH radical, Flavonoid, Polyphenol, 3T3-L1 preadipocyte cell
참고문헌 (32)
- 부희옥, "Assessment on Antioxidant Potential and Enzyme Activity of Some Economic Resource Plants", 한국자원식물학회지, 25(3), pp.349~356 (2012), 한국자원식물학회[DOI]
- 부희옥, "Immune Cells Activity, Nitrite Scanvenging and ABTS Radical Scavenging Activities of Codonopsis lanceolata Ethanol Extracts from Districts in Korea", 한국자원식물학회지, 29(3), pp.289~296 (2016), 한국자원식물학회[DOI]
- Brand-Williams, W., "Use of a free radical method to evaluate antioxidant activity", Food Sci. Tech., 28, pp.25~30 (1995)
- Cao, H., "Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin receptor, and glucose transporter 4 in mouse 3T3-L1 adipocytes", Arch Biochem Biophys, 459(2), pp.214~222 (2007)[DOI]
- Cuyacot, A.R., "Cytotoxicity potentials of some medicinal plants in Mindanao, Philippines", Pelagia Research Library, 4(1), pp.81~89 (2014)
- Ejaz, A., "Curcumin inhibits adipogenesis in 3T3-L1 adipocytes and angiogenesis and obesity in C57", BL mice, 2009, pp.139(5), 919~925
- Fu, X.J., "A study on the antioxidant activity and tissues selective inhibition of lipid peroxidation by saponins from the roots of Platycodon grandiflorum", Am. J. Chin. Med., 37(5), pp.967~975 (2009)[DOI]
- Guizani, N., "Nabag (Zizyphus spina-christi) extract prevents aberrant crypt foci development in colons of azoxymethane-treated rats by abrogating oxidative stress and inducing apoptosis", Asian Pac J Cancer Prev, 14, pp.5031~5035 (2013)[DOI]
- Joh, E.H., "Lancemaside A ameliorates colitis by inhibiting NF-jB activation in TNBS-induced colitis mice", Int. J. Colorectal Dis., 25, pp.545~551 (2010)[DOI]
- 김충현, "장생도라지 (Platycodon grandiflorum)의 항산화 활성 평가", 환경독성보건학회지, 25(1), pp.85~94 (2010), 환경독성보건학회
- 김일두, "Quality Characteristics and Antioxidant Potential of Seeds of Native Korean Persimmon Genotypes", 한국자원식물학회지, 30(6), pp.670~678 (2017), 한국자원식물학회[DOI]
- Kintzios, E., "Terrestrial plant-derived anticancer agents and plant species used in anticancer research", Crit. Rev. Plant Sci., 25, pp.79~113 (2006)[DOI]
- Li, X, "Effect of substrate stiffness on the functions of rat bone marrow and adipose tissue derived mesenchymal stem cells in vitro", J. Biomed. Mater. Res, 102(4), pp.1092~1101 (2014)[DOI]
- Lobo, R., "Curcuma zedoaria Rosc. (white turmeric): a review of its chemical, pharmacological and ethnomedicinal properties", J. Pharm. Pharmacol., 61, pp.13~21 (2009)[DOI]
- Marja P. K., "Antioxidant activity of plant extracts containing phenolic compounds", J. Agri. Food Chem, 47, pp.3954~3962 (1999)[DOI]
- Park, H.J., "Apoptotic effect of hesperidin through caspase 3 activation in human colon cancer cells, SNU-C4", Phytomedicine, 15, pp.147~151 (2008)[DOI]
- Rahul, B.B., "Pancreatic lipase inhibitors from natural sources: unexplored potential", Drug Discov. Today, 12, pp.879~889 (2007)[DOI]
- Rice-Evans, C., "Bramley and J.B. Pridham. 1995. The relative antioxidants activities of plant-derived polyphenolic flavonoids", Free Radic. Res, 22, pp.375~383 (1995)[DOI]
- Roh, C.H., "Screening of crude plant extracts with anti-obesity activity", Int. J. Mol. Sci., 13(2), pp.1710~1719 (2012)[DOI]
- Ryu, C.S., "Evaluation of the total oxidant scavenging capacity of saponins isolated from Platycodon grandiflorum", Food Chem, 132(1), pp.333~337 (2012)[DOI]
- Sanchez-Moreno, C., "Free radical scavenging capacity and inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents", Food Res. Int., 32, pp.407~412 (1999)[DOI]
- Shahidi, F., "Phenolic antioxidants: criteria review", Food Science and Nutrition, 32, pp.67~103 (1992)
- Singleton, V., "Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents", Am. J. Enology Vitic., 16, pp.144~158 (1965)
- Sugihara, N., "Anti and pro-oxidative effects of flavonoids on metal induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes located with "- linolenic acid", Free Radic. Biol. Med., 27, pp.1313~1323 (1999)[DOI]
- Velioglu, Y.S., "Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products", J. Agric. Food. Chem., 46, pp.4113~4117 (1998)[DOI]
- Vijayarathna, S., "Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines", Asian Pac. J. Trop Biomed., 2(10), pp.826~829 (2012)[DOI]
- Wang, L., "Codonopsis lanceolata extract induces G0", G1 arrest and apoptosis in human colon tumor HT-29 cells - Involvement of ROS generation and polyamine depletion, 2011, pp.49, 149~154
- Yan-Wei, H., "Induction of apoptosis in human hepa- tocarcinoma SMMC-7721 cells in vitro by flavonoids from Astragalus complanatus", J. Ethnopharmacol, 123, pp.293~301 (2009)[DOI]
- Yokozawa, T., "Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2-picrylhydrazyl radical", Biochem. Phamacol, 56, pp.213~222 (1998)[DOI]
- Zhishen, J., "The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals", Food Chem., 64, pp.555~559 (1999)[DOI]
- Zhou, K., "Total phenolic contents and antioxidant properties of commonly consumed vegetables grown in Colorado", LWT, 39, pp.1155~1162 (2006)[DOI]
- Zhu, N, "Identification of reaction products of (-)-epigallocatechin, (-)-epigallocatechin gallate and pyrogallol with 2,2-diphenyl-1-picrylhydrazyl radical", Food Chem, 73, pp.345~349 (2001)[DOI]
