구아바(Psidium guajava L.) 잎의 항산화 활성 및 tyrosinase 저해효과
Antioxidant activities and tyrosinase inhibitory effects of guava (Psidiumguajava L.) leaf
2
조회
0
다운로드
14
인용
0
참고문헌
초록
본 연구는 기능성소재 개발에 이용될수 있는 유용한 기 초자료를 얻고자 구아바 잎의 총페놀화합물의 함량, 항산 화활성, tyrosinase저해활성을 측정하였다. 1. 구아바잎 추출물의 총페놀화합물은 19.0(g/100 g, D.W.)의 함량으로 강한 항산화활성을 보였다(radical 소거활성의 IC50는 102.5μg/ml, SOD 유사활성의 IC50는 49.4μg/ml). 2. 용매분획물의radical 소거활성을측정한결과, 100 μg/ml의 농도에서도 ethylacetate(G-E), butanol(G-B), 물분획 (G-W)은 50% 이상의 높은 소거활성을 보였다. radical 소거 활성은 G-B> G-E> G-W 순으로 높은 활성을 보였고, 각 분획물의 농도가 증가할수록 소거능이 높아지는 경향을 나 타냈다. hexane분획(G-H)의 소거활성은 보이지 않았다. 3. 용매분획별 SOD 유사활성에 대한 결과, 100 μg/ml의 농도에서 G-E, G-B, G-W는 각각 81.8, 84.7, 65.3%로 높은 활성을 나타냈으며, 각 분획물의 농도가 증가할수록 SOD 유사활성도 증가하는 경향을 나타냈다. G-H의 활성은 보이지 않았다. 4. 구아바잎의 추출물과 용매분획물의 tyrosinase저해 효과을 측정한 결과, 추출물1 mg/ml의 농도에서 60.8%의 저해효과을 보였으며,용매분획은G-E(65.2%), G-B(62.8%), G-W(51.6%), G-H(28.9%)의 순으로 tyrosinase 저해효 과을 보였다.
Abstract
This research was designed to investigate the antioxidant activities and tyrosinase inhibitory effects of guava (Psidium guajava L.) leaf. Total phenol content was obtained from guava leaf extract of 19.0 (g/100g, D.W.). The crude extract exhibited significantly antioxidant activities (IC50value 102.5μg/ml, free radical scavenging; 49.4μg/ml, SOD like activity). The crude extract of guava leaf was fractionated into four partition layers; hexane (G-H), ethylacetate (G-E), butanol (G-B) and water (G-W) layer. The extracts of G-E, G-B, G-W showed high radical scavenging activities of over 50% at 100μg/ml. SOD like activities of G-E, G-B, G-W were revealed, as 81.8%, 84.7%, 65.3% at 100μg/ml, while those of G-H did not showed the effectively. The crude extract of guava leaf showed high tyrosinase inhibitory effect as 60.8% at 1mg/ml, the measurement of G-E, G-B, G-W were 65.2%, 62.8%, 51.6% and that of G-H was not effective. These results indicate that useful bioactive substances exist in the guava leaf extracts, especially G-E, G-B. And the guava leaf has the potential of being developed into health related products.
참고문헌 (17)
- Begum S., "Triterpenoids from the leaves of Psidium guajava", Phytochemistry, 61, pp.399~403 (2002)
- Chen H.Y, "Antioxidant activity and free radical-scavenging capacity of extracts from guava leaves", Food Chemistry, 101, pp.686~694 (2007)
- Chen J.T, "Hypoglycemic effect of guava juice in mice and human subjects", Am. J. Chin. Med, 11, pp.74~76 (1983)
- 최선영, "식용 및 약용자원의 에스트로젠 활성과 항산화능 평가", 한국식품과학회지, 37(4), pp.549~556 (2005), 한국식품과학회
- Hsieh C.L., "Preventive effects of guava leaves and its active compounds against α-dicarbonyl compounds-induced blood coagulation", Food Chemistry, 103, pp.528~535 (2007)
- 진영준, "저용량 Streptozotocin으로 유도된 당뇨모델 생쥐에서발효 구아바 잎 추출물의 고혈당 억제 효과", 한국식품과학회지, 38(5), pp.679~683 (2006), 한국식품과학회
- 김은영, "항산화활성을 나타내는 약용식물 소재 탐색", 한국식품과학회지, 36(2), pp.333~338 (2004), 한국식품과학회
- Lozoya X., "Intestinal anti-spasmodic effect of a phytodrug of Psidium guajava folia in the treatment of acute diarrheic disease", J. Ethenopharmacol, 83, pp.19~24 (2002)
- Mercadante A.Z., "Carotenoids from guava (Psidium guajava L.): isolation and structure elucidation", Journal of Agricultural and Food Chemistry, 47, pp.145~151 (1999)
- Miean K.H, "Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants", Journal of Agricultural and Food Chemistry, 49, pp.3106~3112 (2001)
- Misra k, "Chemical components of the fruits of Psidium guajava", Phytochemistry, 7, pp.641~645 (1968)
- Nakamura Y., "Functional food chemistry" (1990), Sankyou Publisher Inc
- OlajideO.A., "Pharmacological studies on the leaf of Psidiumguajava", Fitoterapia, 70, pp.25~31 (1999)
- Prota G, "Progress in the chemistry of melanins and related metabolites", Medicinal Research Reviews, 8(4), pp.525~556 (1988)
- Tanaka T., "Tannins and related compounds. CXVI. Six new complex tannins, guajavins, psidinins and psiguavin from the bark of Psidium guajava L", Chem. Pharm. Bull, 40, pp.2092~2098 (1992)
- Wang K.H., "Cosmetic applications of selected traditional Chinese herbal medicines", Journal of Ethnopharmacology, 106, pp.353~359 (2006)
- 유익동, "천연물유래항산화 기능성 화장품 신소재 개발", 대한화장품학회지, 25(2), pp.349~357 (2005)