구아바 열매와 잎 열수 추출물의 신경세포 보호효과
Neuronal Cell Protective Effects of Hot Water Extracts from Guava (Psidium guajava L.) Fruit and Leaf
2
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
PC12 neuronal cell-protective effects of hot water extracts of guava fruit and leaf were evaluated. Total phenolic levels in fruit and leaf were 11.75 and 293.25 mg/g, respectively. Gallic acid, the predominant phenoic, was detected in both extracts. Intracellular reactive oxygen species (ROS) accumulation after H2O2 treatment was significantly reduced when the hot water extract of guava leaf was added to cell medium, compared to PC12 cells treated with H2O2 only. In a cell viability assay using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl- tetrazoliumbromide (MTT), the hot water extracts of fruit and leaf protected against H2O2-induced neurotoxicity. The leaf extract was more effective in terms of inhibition of lactate dehydrogenase (LDH) release into medium, compared to the fruit extract. These in vitro data suggest that hot water extracts of guava fruit and leaf may be useful in treatment of neurodegenerative conditions such as Alzheimer’s disease.
Abstract
PC12 neuronal cell-protective effects of hot water extracts of guava fruit and leaf were evaluated. Total phenolic levels in fruit and leaf were 11.75 and 293.25 mg/g, respectively. Gallic acid, the predominant phenoic, was detected in both extracts. Intracellular reactive oxygen species (ROS) accumulation after H2O2 treatment was significantly reduced when the hot water extract of guava leaf was added to cell medium, compared to PC12 cells treated with H2O2 only. In a cell viability assay using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl- tetrazoliumbromide (MTT), the hot water extracts of fruit and leaf protected against H2O2-induced neurotoxicity. The leaf extract was more effective in terms of inhibition of lactate dehydrogenase (LDH) release into medium, compared to the fruit extract. These in vitro data suggest that hot water extracts of guava fruit and leaf may be useful in treatment of neurodegenerative conditions such as Alzheimer’s disease.
참고문헌 (28)
- Runyons CR, "Antioxidants for prevention of dementia : overview of the pre-advise trial", Alzheimer's Dementia, 1, pp.74~ (2005)
- Behl C, "Alzheimer's disease and oxidative stress: implications for novel therapeutic approaches", Prog Neurobiol, 57, pp.301~323 (1999)
- Ames BN, "Oxidant, antioxidants, and the degenerative disease of aging", Proc Natl Acad Sci USA, 90, pp.7915~7922 (1993)
- Heo HJ, "Effects of banana, orange, and apple on oxidative stress-induced neurotoxicity in PC 12 cells", J FOOD SCI, 73(2), pp.H28~H32 (2008), IFT
- Begum S, "Triterpenoids from the leaves of Psidium guajava", Phytochemistry, 61, pp.399~403 (2002)
- Lozoya X, "Intestinal anti-spasmodic effect of a phytodrug of Psidium guajava folia in the treatment of acute diarrheic disease", J Ethnopharmacol, 83, pp.19~24 (2002)
- Misra K, "Chemical components of the fruits of Psidium guajava", Phytochemistry, 7, pp.641~645 (1968)
- Mercadante AZ, "Carotenoids from guava (Psidium guajava L.) : isolation and structure elucidation", J Agric Food Chem, 47, pp.145~151 (1999)
- Miean KH, "Flavonoid (myricetin, quercetin kaempferol, luteolin, and apigenin) content of edible tropical plants", J Agric Food Chem, 49, pp.3106~3112 (2001)
- Jaiarj P, "Guava leaf extract and topical haemostasis", Phytother Res, 14, pp.388~391 (2000)
- 진영준, "저용량 Streptozotocin으로 유도된 당뇨모델 생쥐에서발효 구아바 잎 추출물의 고혈당 억제 효과", 한국식품과학회지, 38(5), pp.679~683 (2006), 한국식품과학회
- 노상근, "Antidiabetic Effects of Leaves Extracts of Psidium guajava L. and Lagerstroemia speciosa L. in STZ-induced Rats", 생명과학회지, 19(1), pp.40~45 (2009), 한국생명과학회
- 권중호, "구아바 잎의 가공방법에 따른 품질특성", 한국식품저장유통학회지, 14(6), pp.605~610 (2007), 한국식품저장유통학회
- 박병재, "구아바(Psidium guajava L.) 잎의 항산화 활성 및 tyrosinase 저해효과", 한국자원식물학회지, 21(5), pp.408~412 (2008), 한국자원식물학회
- 허예진, "한국산 구아바 잎 추출물을 첨가한 크래커의 항산화활성", 한국식품조리과학회지, 26(2), pp.171~179 (2010), 한국식품조리과학회
- 조영홍, "식중독 세균에 대한 구아바 부위별 추출물의 항균 특성", 한국식품영양과학회지, 38(12), pp.1773~1778 (2009), 한국식품영양과학회
- Hsieh CL, "Molecular action mechanism against apoptosis by aqueous extract from guava budding leaves elucidated with human umbilical vein endothelial cell (HUVEC) model", J Agric Food Chem, 55, pp.8523~8533 (2007)
- Kim DO, "Antioxidant capacity of phenolic phytochemical from various cultivars of plums", Food Chem, 81, pp.321~326 (2003)
- Heo HJ, "Protective effect of 4',5- dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica against Aβ-induced oxidative stress in PC12 cells", Amyloid, 8, pp.194~201 (2001)
- 곽지현, "자색고구마 추출물의 항산화 효과 및 신경세포 보호효과", 농업생명과학연구, 44(2), pp.57~66 (2010), 농업생명과학연구원
- Chen HY, "Antioxidant activity and free radical-scavenging capacity of extracts from guava (Psidium guajava L.) leaves", Food Chem, 101, pp.686~694 (2007)
- Thaipong K, "Comparison of ABTS, DPPH, FRAP, and ORAC assays fro estimating antioxidant activity from guava fruit extracts", J Food Compost Anal, 19, pp.669~675 (2006)
- Natitanon W, "Factors influencing antioxdiant activities and total phenolic contents of guava leaf extract", LWT-Food Sci Technol, 43, pp.1095~1103 (2010)
- Zhao B, "Natural antioxidants protect neurons in Alzheimer's disease and Parkinson's disease", Neurochem Res, 34, pp.630~638 (2009)
- Heo HJ, "Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells", J Agric Food Chem, 53, pp.1984~1989 (2005)
- Heo HJ, "Effects of banana, orange, and apple on oxidative stress-induced neurotoxicity in PC12 cells", J Food Sci, 73, pp.28~32 (2008)
- Heo HJ, "Apple phenolics protect in vitro oxidative stress-induced neuronal cell death", J Food Sci, 69, pp.357~360 (2004)
- Wu JW, "Inhibitory effects of guava (Psidium guajava L.) leaf extracts and its active compounds on the glycation process of protein", Food Chem, 113, pp.78~84 (2009)