롱빈 콩 에탄올추출물의 항산화 및 NO 생성 억제 효과
Antioxidant and NO Production Inhibitory Effects of Longbean Ethanol Extract
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
.
Abstract
In order to prepare basic data for examining the development of longbean (Vigna unguiculata ssp. sesquipedalis) as a possible natural material for functional cosmetics, the study investigated the yield, total polyphenol content, antioxidant, and the NO production inhibitory effects of an ethanol-produced extract of longbean soybean parts. As a result, the yield of the longbean ethanol extract was found to be 14.93% and the total polyphenol content was confirmed to be 4.36±0.473 mg/g. When the antioxidant effect was measured by the electron donating ability and the ABTS radical scavenging activity, the electron donating ability tended to increase as the concentration increased, and showed a high scavenging ability of 92.32% at a concentration of 1,000 μg/mL. And the ABTS radical scavenging activity showed a scavenging effect of 99.81% at a concentration of 100 μg/ml, and the scavenging effect was equivalent to that of vitamin C. As a result of measuring the antioxidant activity, the ABTS radical scavenging ability showed a higher scavenging effect than the electron donating ability. The cell viability of RAW 264.7 cells was 99.82-100.28% up to the concentration of 500 μg/ml, and cytotoxicity was not observed up to 500 μg/ml. The NO production inhibitory activity showed a significant decrease to 81.84% at a concentration of 500 μg/ml. Therefore, it is considered that the long bean ethanol extract can be used as a natural material for cosmetics. In future studies, it is necessary to demonstrate the physiological activity, such as anti-inflammatory mechanisms, whitening, and wrinkle improvement through component analysis.
참고문헌 (43)
- 강명주, "업사이클링 화장품에 대한 대학생 소비자의 태도 및 구매행동", 한국화장품미용학회지, 9(3), pp.417~427 (2019), 한국화장품미용학회
- 박서연, "천연 화장품에 대한 인식과 사용실태 및 만족도에 관한 연구", 석사, pp.2020
- 박영환, "견우자추출물의 화장품소재로서의 응용 연구", 석사, pp.2022
- 배준태, "생물전환기술을 이용한 감초추출물의 주름개선 화장품 소재 개발", 박사, pp.2020
- 김대용, "브로콜리 추출물의 향장활성 효과", 한국인체미용예술학회지, 17(1), pp.29~39 (2016), 한국인체미용예술학회
- 강보라, "천연물 소재 기술기업의 사업화 계획 및 비즈니스 모델 수립", 석사, pp.2023
- 김영훈, "천연소재의 화장품약리활성에 관한 연구", 한국패션뷰티학회지, 5(3), pp.99~104 (2007)
- 심승보, "지역 특산 천연소재의 특성 분석과 화장품 제형에서의 효능 연구", 박사, 화학공학과, pp.2012
- 이건희, "생물전환 콩 배아 추출물의 소재 특성 및 수면 팩 효능 연구", 석사, pp.2019
- J. A. Kim, "Comparison of isoflavones composition in seed, embryo, cotyledon and seed coat of cookedwith-rice and vegetable soybean (Glycine max L.) varieties", Food Chem, 102(3), pp.738~744 (2007)
- 김정민, "콩 이소플라본의 기능및 연구현황", 콩산업정보지, 6, pp.22~30 (2023)
- L. Ravanti, "Induction of collagenase-3(MMP-13)expression in human skin fibroblasts by three-dimensional collagen is mediated by p38 mitogen-activated protein kinase", JBC, 274(4), pp.2446~2455 (1999)
- Y. Wang, "Inhibition of ultraviolet B(UVB)-induced c-fos and c-jun expression in vivo by a tyrosine kinase inhibitor genistein", Carcinogenesis, 19(4), pp.649~654 (1998)
- 이길홍, "대두 이소플라본인 제니스테인의 항산화적 성질과 미백화장품에의 응용", 석사, 정밀화학, pp.2004
- "농촌진흥청, 지역별 ‘아열대작물’ 재배 가능성 평가" (2018)[URL]
- 정영빈, "노지재배시 롱빈 정식시기에 따른생육 및 수량 특성", 원예과학기술지 추계학술발표회자료집, 34(2), pp.61~ (2016)
- 권혜정, "콩과 아열대 채소(롱빈, 그린빈)품종별 품질 특성 비교", 한국식품영양과학회학술대회발표집, pp.269~ (2020)
- 하태정, "다이오드어레이 검출 및 전기 분무 이온화", 질량 분석법(HPLC-DAD-ESI, 농업 및 식품화학 저널, pp.2010, 58(4)[DOI]
- 한인범, "불림 건조 가공 방법에 따른 콩의이소플라본 함량 변화", 한국식품영양과학회학술대회발표집, pp.410~ (2021)
- 김미경, "블루베리 잎 추출물의 항산화 및 항염증 효과", 한국인체미용예술학회지, 15(4), pp.109~120 (2014), 한국인체미용예술학회
- V. L. Sigleton, "Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents", Am J Enol Viticult, 16(3), pp.144~158 (1965)
- M. S. Blois, "Antioxidant determinations by the use or a stable free radical", Nature, 181, pp.1199~1200 (1958)
- N. Pellegrini, "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2, 2'-azinobis(3-ethylenebenzothazoline-6-sulfonic acid radical cation decolorization assay", Method Enzymol, 299, pp.379~384 (1999)
- R. Re, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Rad Biol Med, 26(9-10), pp.1231~1237 (1999)
- J. Carmichael, "Evaluation of a Tetrazolium-based Semiautomated Colorimetric Assay-Assessment of Chemosensitivity Testing", Cancer Res, 47(4), pp.936~942 (1987)
- Nitrate in Biological Fluids, "Anal Biochem", 1982, 131~138
- N. R. Perron, "A review of the antioxidant mechanisms of polyphenol compounds related to iron binding", Cell Biochem Biophys, 53(2), pp.75~100 (2009)
- L. O. Dragsted, "Antioxidant actions of polyphenols in humans", Int J Vitam Nutr Res, 73(2), pp.112~119 (2003)
- 주은영, "검은콩의 품종에 따른 콩과 청국장 추출물의 항산화능 및 혈전용해능", Food Science and Preservation, 17(6), pp.874~880 (2010), 한국식품저장유통학회
- 주은영, "대두와 청국장 추출물의 항산화능과 혈전용해능", Food Science and Preservation, 18(6), pp.930~937 (2011), 한국식품저장유통학회
- K. Tawaha, "Antioxidant activity and total phenolic content of selected Jordanian plant species", Food Chem, 104(4), pp.1372~1378 (2007)
- I. Gulcin, "Antiradical and antioxidant activity of total anthocyanins from Perilla pankinensis decne", J Ethmopharmacol., 101, pp.287~293 (2005)
- 김은진, "자생식물과 생약자원 추출물의 폴리페놀, 플라보노이드 함량 및 항산화 활성 탐색", 한국식품과학회지, 44(3), pp.337~342 (2012), 한국식품과학회[DOI]
- 김미경, "추출방법에 따른 Neem의 항산화, 항균 활성 및 항염 효과 비교", 한국인체미용예술학회지, 20(3), pp.265~278 (2019), 한국인체미용예술학회[DOI]
- D. B. R. Van, "Applicability of an improved Trolox equivalent antioxidant capacity(TEAC)assay for evaluation of antioxidant capacity measurements of mixtures", Food Chem, 66(4), pp.511~517 (1999)
- J. M. Awika, "Screening methods to measure antioxidant activity of sorghum(Sorghum bicolor)and sorghum products", J Agric Food Chem, 51, pp.6657~6662 (2003)
- 명정은, "품종별 콩 추출물의 주요 성분 분석 및 항산화 효과", 한국콩연구회지, 25(1), pp.23~29 (2008)
- 리혁, "컨베이어 원적외선 건조기를 이용한 표고버섯의 건조 및 항산화 특성", 한국식품영양과학회지, 36(2), pp.250~254 (2007), 한국식품영양과학회
- N. McCartney-Francis, "Suppression of arthritis by an inhibitor of nitric oxide synthase", J Exp Med, 178(2), pp.749~754 (1993)
- A. Weisz, "Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferongamma, bacterial lipopolysaccharide and NGmonomethyl-L-arginine", Biochem J, 316, pp.209~215 (1996)
- D. C. Jenkins, "Roles of nitric oxide in tumor growth", Proc Natl Acad Sci USA, 92(10), pp.4392~4396 (1995)
- 임지영, "녹두 및 대두추출물의 항암 및 항염증 활성", 한국식품과학회지, 42(6), pp.755~761 (2010), 한국식품과학회
- H. K. Kim, "Effects of narally occurring flavonoids on nitric oxide production in the macrophage cell line RAW264.7 and their structureñactivity relationships", Biochem Pharmacol, 58(5), pp.759~765 (1999)
