밀싹 에탄올 추출물의 항산화 및 항염증 활성
Antioxidant and Anti-inflammatory Activities of Ethanol Extracts from Wheat Sprout
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본 연구는 밀싹 에탄올 추출물의 항산화 및 항염증 효과를 확인하였다. 밀싹 에탄올 추출물은 radical 소거능을 갖고 있었 으며, 10 mg/mL 농도에서 폴리페놀 함량 11.7 mg/100 g, 플라보 노이드 함량 8 mg/100 g로 항산화 활성이 우수한 것으로 확인되 었다. 항염증 효과를 확인하고자 RAW 264.7 세포에 LPS를 처 리하여 NO 생성 억제를 확인한 결과, 밀싹 에탄올 추출물은 뛰 어난 NO 생성 억제능이 있었으며, 25 μg/mL의 농도에서 80% 의 높은 NO 생성 억제를 보였다. 또한 RAW 264.7 세포에서 현 저한 COX-2 단백질 발현 억제와 약한 iNOS mRNA 발현 억제 작용을 확인하였다. 따라서, 밀싹 에탄올 추출물은 항산화, 항염 증 효과를 가진 기능성 화장품 소재로써의 활용이 가능할 것으 로 사료된다.
Abstract
Wheat sprout refers to young sprout of wheat. Wheat sprout is rich in nutrients such as amino acid, mineral, vitamins, and chlorophyll and it has been known that various active ingredients including calcium and potassium increase during the growth of wheat sprout. Currently, much attention has been drawn to natural extracts that have little side effects. By studying wheat sprout that is not toxic and has abundant nutrients, this research aims to provide basic materials for functional cosmetics that have anti-oxidant and anti-inflammatory effects. In order to verify the anti-oxidation effect, DPPH radical elimination ability was measured, which confirmed the DPPH radical elimination ability of wheat sprout extract. When the total content of polyphenol and flavonoid of wheat sprout ethanol extract was measured, high content of polyphenol and flavonoid was verified with 11.7 mg/100 g of polyphenol and 8 mg/100 g of flavonoid in 10 mg/mL concentration. RAW 264.7 cell was processed by lipopolysaccharide to induce nitric oxide, and the inhibition rates of nitric oxide formation and COX-2 protein expression were examined. This demonstrated that nitric oxide formation and expression of COX-2 protein were significantly inhibited at 25 μg/mL concentration of wheat sprout ethanol extract. Through these results, wheat sprout ethanol extract had an excellent antioxidant activity. Also, it effectively inhibited the formation of nitric oxide and expression of COX-2 protein that cause inflammation. This shows that wheat sprout ethanol extract can be used as an ingredient for functional cosmetics with anti-aging and anti-oxidation effects.
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