葫蘆巴추출물의 Peroxynitrite 제거 및 염증 인자 단백질 억제 효과
Effect of Trigonellae Semen on Peroxynitrite Scavenging Activity and Protein Expression of Proinflammatory Mediators
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Abstract
Objectives : Peroxynitrite (ONOO-), O2- and nitric oxide (NO) are cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer's disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate ONOO- scavenging activities, and that of its precursors, NO and O2- of Trigonellae Semen. Methods : To investigate ONOO-, NO, O2- scavenging activities, fluorescent probes, namely 2’,7’- dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Protein expression levels of iNOS, COX-2, NF-κB, and VCAM-1 were assayed by western blot. Results : Trigonellae Semen markedly scavenged authentic ONOO-, O2- and NO. It also inhibited ONOO- induced by O2- and NO which are derived from SIN-1. Furthermore, Trigonellae Semen inhibited ONOO-, O2- and NO generation in LPS-treated ICR mouse kidney postmitochondria. Trigonellae Semen inhibited gene expression of iNOS, COX-2, VCAM-1 and NF-κB (p65) activation. Conclusions : These results suggest that Trigonellae Semen is an effective ONOO-, O2- and NO scavenger, and that this substance has a potential role as an inhibitor of aging process, and in therapy against age-related diseases.
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