싸리나무 줄기 추출물의 추출 용매에 따른 항산화활성과 Nitric Oxide 생성 억제 활성
Antioxidative and Nitric Oxide Production Inhibitory Activities of Lespedeza bicolor Stem Extracts Depending on Solvents
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In this study, we investigated on antioxidative activity and nitric oxide production inhibitory activity of various solvent extracts of Lespedeza bicolor. The total polyphenol content of the methanol extract was 192.6㎎/g and flavonoid content of the acetone extract was 40.6㎎/g, as the highest content. In DPPH radical scavenging ability, SC_50 values of the ethanol and methanol extract were exhibited 0.69 ㎎/㎖ and 0.89 ㎎/㎖, respectively. However, in nitric oxide(NO) scavenging ability, SC50values of the acetone was exhibited 0.72 ㎎/㎖ as the highest activity. Moreover, the acetone extract showed strong NO production inhibitory effect in lipopolysaccharide(LPS)-stimulated Raw 264.7 cell. In the cytotoxicity measurement by MTT assay, the extracts were exhibited Raw 264.7 cell viabilities of 92.57~129.04% as nontoxic result in concentration of 65~650 ㎍/㎖. As a result, the acetone extract of L. bicolor could be applicable to functional materials for anti-inflammatory related fields.
Abstract
In this study, we investigated on antioxidative activity and nitric oxide production inhibitory activity of various solvent extracts of Lespedeza bicolor. The total polyphenol content of the methanol extract was 192.6㎎/g and flavonoid content of the acetone extract was 40.6㎎/g, as the highest content. In DPPH radical scavenging ability, SC_50 values of the ethanol and methanol extract were exhibited 0.69 ㎎/㎖ and 0.89 ㎎/㎖, respectively. However, in nitric oxide(NO) scavenging ability, SC50values of the acetone was exhibited 0.72 ㎎/㎖ as the highest activity. Moreover, the acetone extract showed strong NO production inhibitory effect in lipopolysaccharide(LPS)-stimulated Raw 264.7 cell. In the cytotoxicity measurement by MTT assay, the extracts were exhibited Raw 264.7 cell viabilities of 92.57~129.04% as nontoxic result in concentration of 65~650 ㎍/㎖. As a result, the acetone extract of L. bicolor could be applicable to functional materials for anti-inflammatory related fields.
참고문헌 (29)
- Baek, "Inhibitory Effect of Dalbergioidin Isolated from the Trunk of Lespedezacyrtobotrya on Melanin Biosynthesis", Journal of Microbiology and Biotechnology, 18(5), pp.874~879 (2008), 한국미생물·생명공학회
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Brune B, "Nitric oxide, oxidative stress and apoptosis", Kidney International Supplement, 84, pp.4~22 (2003)
- 김상찬, "현삼메탄올 추출물이 LPS로 유도된Raw 264.7 cell에서의 TNF-α, IL-1β, IL-6 및 nitric oxide 생성에 미치는 영향", 대한본초학회지, 20(2), pp.7~16 (2005), 대한본초학회
- Chung HT, "Nitric oxide as a bioregulator of apoptosis", Biochemical and Biophysical Research Communications, 282, pp.1075~1079 (2001)
- Ding AH, "Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production", The Journal of Immunology, 141, pp.2407~2412 (1988)
- 엄효진, "건조온도에 따른 비파 잎의 생리활성 변화", 생약학회지, 40(3), pp.178~183 (2009), 한국생약학회
- Fridovich I, "Superoxide dismutase an adaption to paramagnetic gas", Journal of Biological Chemistry, 264, pp.7761~7762 (1989)
- 임도연, "흰민들레 에탄올 추출물 및 분획물의 항산화 및 항균활성과 Tyrosinase 저해활성", 한국약용작물학회지, 19(4), pp.238~245 (2011), 한국약용작물학회
- 정용준, "상황버섯과 발아현미상황버섯 열수추출물의 Murin Macrophage RAW 264.7 세포에서 항염증 반응 비교", 한국약용작물학회지, 17(2), pp.97~101 (2009), 한국약용작물학회
- Jung SW, "Screening of tyrosinase inhibitor from plants", Korean Journal of Food Science and Technology, 27, pp.891~896 (1995)
- Kang SS, "Natural products sciences", pp.289~ (1998), Seoul National University
- Lee A, "Studies on the antioxidantive and antimicrobial effects of Lespedeza bicolor extracts", Journal of Korean Society of Esthetic & Cosmeceutics, 1, pp.109~120 (2006)
- Lee CH, "The pharmacology of Chinese herbs", pp.222~ (1993), CRC press
- Lee ES, "Inhibition of nitric oxide and tumor necrosis factor production by propenone compound through blockade of nuclear factor-B activation in cultured murine macrophages", BIOLOGICAL & PHARMACEUTICAL BULLETIN, 27(5), pp.617~620 (2004), PHARMACEUTICAL SOC JAPAN
- 김남우, "싸리(Lespedeza bicolor) 추출물의 항산화성에 관한 연구", 한국식품저장유통학회지, 12(1), pp.75~79 (2005), 한국식품저장유통학회
- 김남우, "싸리 추출물의 폴리페놀 함량과 생리활성", 한국식품저장유통학회지, 13(5), pp.616~622 (2006), 한국식품저장유통학회
- Liu RH, "Potential synergy of phytochemicals in cancer prevention: mechanism of action", The Journal of Nutrition, 134, pp.3479S~3485S (2004)
- Manach C, "Bioavailability and bioefficacy of polyphenols in humans", The American Journal of Clinical Nutrition, 81, pp.23000S~242S (2005)
- Marcocci L, "The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761", Biochemical and Biophysical Research Communications, 201, pp.748~755 (1994)
- Mitsuhashi H, "In illustrated medicinal plants of the world in color", pp.220~ (1998), Hokuryukan
- Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several region of Argentina", Journal of Ethnopharmacology, 71, pp.109~114 (2000)
- 오영지, "천궁(Cnidium officinale Makino) 지상부 추출물의 항산화 활성 평가", 한국약용작물학회지, 18(6), pp.373~378 (2010), 한국약용작물학회
- 유인식, "싸리꽃 에탄올 추출물의 皮膚 生理活性 효과", 한방안이비인후피부과학회지, 20(2), pp.1~9 (2007), 대한한방안이비인후피부과학회
- Dong Ha Cho, "Changes in Total Polyphenol, Total Flavonoid Contents andAntioxidant Activities of Hibiscus cannabinus L.", 한국약용작물학회지, 14(5), pp.307~310 (2006), 한국약용작물학회
- 신경민, "Amygdalin의 Murine Macrophage Raw 264.7 세포에서 in vitro 항염효과", 생약학회지, 34(3), pp.223~227 (2003), 한국생약학회
- Videla LA, "Biochemical aspects of cellular oxidative stress", Archivos de Biología y Medicina Experimentales, 21, pp.85~92 (1988)
- 윤유식, "연근별 황기의 항산화 및 항당뇨 활성 평가 고찰", 생약학회지, 40(1), pp.1~5 (2009), 한국생약학회
- 윤태숙, "Evaluation of Solvent Extraction on the Anti-Inflammatory Efficacy of Glycyrrhiza uralensis", 한국약용작물학회지, 18(1), pp.28~33 (2010), 한국약용작물학회