麻杏甘石湯加減方이 천식모델생쥐의 면역세포 및 사이토카인에 미치는 영향
Effects of Mahaenggamseok-tang-gagambang on Immune Cells and Cytokines in OVA-Induced Asthmatic Mice
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The purpose of this research is to evaluate the effect of Mahaenggamseok-tang-gagambang (MGTG) on airway hyper- responsiveness (AHR), immune cells, cytokines and lung tissue in OVA-induced asthmatic mice. C57BL/6 mice were injected, inhaled and sprayed with OVA for 12 weeks (3times a week) for asthma sensitization and challenge. Two experimental groups were treated with different concentrations of MGTG (400 ㎎/㎏ and 200 ㎎/㎏) extract and cyclosporin A (10 ㎎/㎏) for the later 8 weeks. Enhanced pause (Penh) levels were measured by whole body plethysmography. Immune cells were analyzed by flow cytometer in peripheral blood monocyte cell (PBMC) and lung cells. The IL-1b, IL-12, IFN-γ, OVA-IgE, IL-4, IL-5, TNF-α were analyzed by ELISA kit in serum and splenocyte+a-cCD3/a-CD28. Enhanced pause (Penh) levels of the MGTG groups (400 ㎎/㎏ and 200 ㎎/㎏) were decreased significantly compared with that of control group. The numbers of MGTG groups (400 ㎎/㎏ and 200 ㎎/㎏) on lung total cells were decreased significantly compared with that of control group. The numbers of MGTG groups (400 ㎎/㎏ and 200 ㎎/㎏) on CD3+/CD69+, B220+/CD22+, B220+/CD23+, B220+/IgE+, CCR3+ cells were decreased significantly compared with that of control group. The number of MGTG group (400 ㎎/㎏) on CD3+/CD49b+ cells was decreased significantly compared with that of control group. The level of MGTG groups (400 ㎎/㎏ and 200 ㎎/㎏) on IL-4, IL-5, IL-12, TNF-α, OVA-IgE were decreased significantly compared with that of control group. The level of MGTG group (400 ㎎/㎏) on IL-1b, IL-13, OVA-IgE were decreased significantly compared with that of control group. These results demonstrate that MGTG could be a desirable alternative therapy for allergic asthma by inhibiting the expression of immune cells, the activation of inflammatory mediator.
Abstract
The purpose of this research is to evaluate the effect of Mahaenggamseok-tang-gagambang (MGTG) on airway hyper- responsiveness (AHR), immune cells, cytokines and lung tissue in OVA-induced asthmatic mice. C57BL/6 mice were injected, inhaled and sprayed with OVA for 12 weeks (3times a week) for asthma sensitization and challenge. Two experimental groups were treated with different concentrations of MGTG (400 ㎎/㎏ and 200 ㎎/㎏) extract and cyclosporin A (10 ㎎/㎏) for the later 8 weeks. Enhanced pause (Penh) levels were measured by whole body plethysmography. Immune cells were analyzed by flow cytometer in peripheral blood monocyte cell (PBMC) and lung cells. The IL-1b, IL-12, IFN-γ, OVA-IgE, IL-4, IL-5, TNF-α were analyzed by ELISA kit in serum and splenocyte+a-cCD3/a-CD28. Enhanced pause (Penh) levels of the MGTG groups (400 ㎎/㎏ and 200 ㎎/㎏) were decreased significantly compared with that of control group. The numbers of MGTG groups (400 ㎎/㎏ and 200 ㎎/㎏) on lung total cells were decreased significantly compared with that of control group. The numbers of MGTG groups (400 ㎎/㎏ and 200 ㎎/㎏) on CD3+/CD69+, B220+/CD22+, B220+/CD23+, B220+/IgE+, CCR3+ cells were decreased significantly compared with that of control group. The number of MGTG group (400 ㎎/㎏) on CD3+/CD49b+ cells was decreased significantly compared with that of control group. The level of MGTG groups (400 ㎎/㎏ and 200 ㎎/㎏) on IL-4, IL-5, IL-12, TNF-α, OVA-IgE were decreased significantly compared with that of control group. The level of MGTG group (400 ㎎/㎏) on IL-1b, IL-13, OVA-IgE were decreased significantly compared with that of control group. These results demonstrate that MGTG could be a desirable alternative therapy for allergic asthma by inhibiting the expression of immune cells, the activation of inflammatory mediator.
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